Addgene

We narrowed to 7,193 results for: Trac;

Showing: 1721 - 1740 of 7193 results

Results per page:

Sort results by:

pBJ7

Plasmid

#167136

Purpose

A tetracyclin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter

Depositor

Akira Mine

Article

Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

Insert

luxCDABE

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

Kanamycin promoter

Available since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD-GINKO1

Plasmid

#113111

Purpose

Bacterial expression of genetically encoded green fluorescent potassium ion indicator GINKO1

Depositor

Robert Campbell

Article

Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

Insert

GINKO1

Use

Tags

6-His Tag, T7 tag (gene 10 leader), and Xpress™ t…

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE30 PTBP1 isoform1

Plasmid

#108591

Purpose

Recombinant His tagged protein production

Depositor

Chris Smith

Article

Wollerton et al RNA. 2001 Jun;7(6):819-32.

Insert

PTBP1 isoform 1 (PTBP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

isoform 1 (isoform c)

Promoter

Available since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEx-deletion-tet

Plasmid

#172214

Purpose

For allelic exchange in tetracyclin-sensitive Prevotella copri strains

Depositor

Till Strowig

Article

Li et al EMBO J. 2021 Dec 1;40(23):e108287. doi:

Type

Empty backbone

Use

Allelic exchange in prevotella copri

Tags

Expression

Mutation

Promoter

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNES-VL12.3

Plasmid

#43862

Depositor

Dane Wittrup

Article

Colby et al Proc Natl Acad Sci U S A. 2004 Dec 21;10

Insert

VL12.3

Use

Tags

His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 4, 2013

Availability

Academic Institutions and Nonprofits only
pX459-SpCas9-NG

Plasmid

#171370

Purpose

SpCas9-NG-mediated genome editing.

Depositor

Masahito Ikawa

Article

Oura et al Commun Biol. 2021 Jun 23;4(1):771. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 for sgRNA; CBh for Cas9-2A-puroR

Available since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
plenti.CamKII.(cyto).iATPSnFR2.A95K.HaloTag

Plasmid

#209726

Purpose

Expresses cytosolic medium affinity ratiometric ATP sensor

Depositor

Timothy Ryan

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

plenti.CamKII.(cyto).iATPSnFR2.A95K.HaloTag

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CamKII

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21b-LaG9(S151C)

Plasmid

#172490

Purpose

Encodes the anti-GFP LaG-9 nanobody with a cysteine near the C-terminus, LaG-9(S151C).

Depositor

Siegfried Musser

Article

Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

Insert

LaG9(S151C)

Use

Tags

6xHis, PPX cleavage site, and pelB leader sequenc…

Expression

Bacterial

Mutation

S151C

Promoter

T7

Available since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pPI138

Plasmid

#113229

Purpose

extrachromosomal expression vector, expression of LifeAct-GFP

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Insert

LifeAct

Use

Dictyostelium discoideum expression vector, extra…

Tags

GFP

Expression

Mutation

Promoter

act15

Available since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pN-PITCh-3XHA

Plasmid

#127881

Purpose

Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter HA tagging

Depositor

Peter Kaiser

Article

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Insert

GFP-Puro-3XHA (PPP2CA Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBluescriptSKII+ U6-sgRNA(F+E) ACTB

Plasmid

#74705

Purpose

Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of ACTB mRNA

Depositor

Eugene Yeo

Article

Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

Insert

U6 promoter driving sgRNA targeting the 3'UTR of ACTB mRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
CRY2high-mCherry

Plasmid

#104063

Purpose

Expresses fusion of CRY2high mutant (CRY2PHR E490R) with mCherry

Depositor

Bianxiao Cui

Article

Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

Insert

CRY2high-mCherry

Use

Tags

Expression

Mammalian

Mutation

CRY2PHR E490R

Promoter

CMV

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTCAV-FLEx(loxP)-FlpO

Plasmid

#67829

Purpose

Cre-dependent expression of FlpO from CAV2 virus

Depositor

Liqun Luo

Article

Schwarz et al Nature. 2015 Aug 6;524(7563):88-92. doi:

Insert

FlpO

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 5HT2A_NB2_1

Plasmid

#127690

Purpose

Plasmid contains a positive allosteric modulating nanobody the binds to the cytoplasmic surface of 5HT2A.

Depositor

Justin English, Bryan Roth

Article

English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

Insert

5HT2A_NB2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pME-PLIN2-tdTOMATO

Plasmid

#172533

Purpose

PLIN2-tdTOMATO fusion (zebrafish lipid droplet reporter) in middle entry vector for Gateway cloning

Depositor

Richard White

Article

Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

Insert

plin2-tdtomato

Use

Tags

Expression

Mutation

S80R, A81S, E144D, I148F, and R415H mutations in …

Promoter

Available since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPy-CAG-Cre::ERT2-IRES-BSD

Plasmid

#48760

Purpose

CAG promoter driving Cre-ERT2 fusion-IRES-Blasticidin resistance

Depositor

Austin Smith

Article

Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

Insert

Cre::ERT2

Use

Ppy

Tags

ERT2

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pPBpuro-COX2-EBFP2

Plasmid

#184720

Purpose

Exogenous COX2

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

COX2-EBFP2 (PTGS2 Synthetic, Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
CRY2low-mCherry

Plasmid

#104065

Purpose

Expresses fusion of CRY2low mutant (CRY2 aa1-491 R489E A491D) with mCherry

Depositor

Bianxiao Cui

Article

Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

Insert

CRY2low-mCherry

Use

Tags

Expression

Mammalian

Mutation

CRY2 aa 1-491 truncation, R489E A491D

Promoter

CMV

Available since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTS2365-Tier1-PhCMV-TetR

Plasmid

#169566

Purpose

Tier-1 vector encoding PhCMV-driven tetracycline dependent regulator protein TetR (PhCMV-TetR-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven tetracycline repressor protein class B from transposon Tn10

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CRY2low-tdTomato

Plasmid

#104067

Purpose

Expresses fusion of CRY2low mutant (CRY2 aa1-491 R489E A491D) with tdTomato

Depositor

Bianxiao Cui

Article

Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

Insert

CRY2low-tdTomato

Use

Tags

Expression

Mammalian

Mutation

CRY2 aa 1-491 truncation, R489E A491D

Promoter

CMV

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge