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Tol2-lyzC-microRNA 212-Dendra2

Plasmid

#97122

Purpose

overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

microRNA 212 Dendra2

Use

Zebrafish

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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Tol2-lyzC-microRNA 214-Dendra2

Plasmid

#97123

Purpose

overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

microRNA 214 Dendra2

Use

Zebrafish

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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Tol2-lyzC-microRNA 216a-Dendra2

Plasmid

#97124

Purpose

overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

microRNA 216a Dendra2

Use

Zebrafish

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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Tol2-lyzC-microRNA 218a-2-Dendra2

Plasmid

#97125

Purpose

overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

microRNA 218a 2 Dendra2

Use

Zebrafish

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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Tol2-lyzC-microRNA Let 7-2-dendra2

Plasmid

#97103

Purpose

overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

microRNA Let 7 2 dendra2

Use

Zebrafish

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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Tol2-lyzC-microRNA 19c-Dendra2

Plasmid

#97104

Purpose

overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

microRNA 19c Dendra2

Use

Zebrafish

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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Tol2-lyzC-microRNA 22b-Dendra2

Plasmid

#97105

Purpose

overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

microRNA 22b Dendra2

Use

Zebrafish

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+-NONHSAT073641

Plasmid

#92432

Purpose

mammalian expression of lncRNA NONHSAT073641

Depositor

Ralf Brandes, Matthias Leisegang

Article

Josipovic et al Acta Physiol (Oxf). 2016 Apr 28. doi: 10

Insert

NONHSAT073641

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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ARK1a

Plasmid

#81016

Purpose

E.coli BW25113 + JBEI-12056 (pBbA5c-MevTo-BBa1002-pTrc-MKco-PMKco, JBx_041105) + JBEI-9348 (pTrc99a-PMDsc-NudB, JBx_039365)

Depositor

Taek Soon Lee

Article

Kang et al Metab Eng. 2016 Mar;34:25-35. doi: 10.10

Inserts

MevTo-BBa1002-pTrc-Mkco-PMKco
PMD-NudB

Promoter

LacUV5 and Trc promoter

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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FH1-PNGT#1-mCherry_PGK-H2B-CFP

Plasmid

#164103

Purpose

Fluorescent reporter for genetic tracing of proneural Glioblastoma cell-state.

Depositor

Gaetano Gargiulo

Article

Schmitt et al Cancer Discov. 2021 Mar;11(3):754-777. d

Insert

PNGT#1-mCherry

Use

Lentiviral and Synthetic Biology

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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FH1-MGT#1-mVenus_PGK-H2B-CFP

Plasmid

#164100

Purpose

Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

Depositor

Gaetano Gargiulo

Article

Schmitt et al Cancer Discov. 2021 Mar;11(3):754-777. d

Insert

MGT#1-mVenus

Use

Lentiviral and Synthetic Biology

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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FH1-CLGT#2-mCherry-_PGK-H2B-CFP

Plasmid

#164106

Purpose

Fluorescent reporter for genetic tracing of classical Glioblastoma cell-state.

Depositor

Gaetano Gargiulo

Article

Schmitt et al Cancer Discov. 2021 Mar;11(3):754-777. d

Insert

CLGT#2-mCherry

Use

Lentiviral and Synthetic Biology

Available Since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pMYH9-D1424H-FLAG

Plasmid

#183513

Purpose

Gateway expression vector encoding D1424H mutant MYH9 cDNA, with N-terminal Flag tag. For mammalian expression.

Depositor

Emanuele Panza

Article

Panza et al Pathogenetics. 2008 Dec 1;1(1):5. doi: 1

Insert

MYH9 (MYH9 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Aspartic acid 1424 to Histidine

Promoter

CMV

Available Since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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SopB(C460S)-EGFP

Plasmid

#183656

Purpose

C-terminally tagged Salmonella Typhimurium SopB (catalytically inactive) for mammalian expression

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Salmonella enterica serovar Typhimurium)

Tags

EGFP

Expression

Mammalian

Mutation

C460S

Promoter

CMV

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pJOP-HTT-HR18Q

Plasmid

#87228

Purpose

HTT HR arms with 18 CAG repeats and piggyBac selection cassette

Depositor

Mahmoud Pouladi

Article

Xu et al Stem Cell Reports. 2017 Feb 21. pii: S22

Insert

1.7kb HTT 5' homology arm, 2.5kb HTT 3' homology arm

Expression

Mammalian

Promoter

EF1a driving selection cassette

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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NA20 LN16

Plasmid

#101870

Purpose

Expresses HIV Env gene when transfected into 293-T cells for production of pseudovirus

Depositor

Paul Clapham

Article

Peters et al J Virol. 2004 Jul;78(13):6915-26.

Insert

Env (env )

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only
EF1A_Hygro_P2A_RUNX1_R118G_Barcode

Plasmid

#170970

Purpose

Overexpression of RUNX1 R118G mutant protein.

Depositor

Prashant Mali

Article

Ozturk et al Cell Rep. 2024 Jul 4;43(7):114436. doi:

Insert

RUNX1 (RUNX1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

R118G

Promoter

Ef1A

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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EF1A_Hygro_P2A_RUNX1_G100V_Barcode

Plasmid

#170955

Purpose

Overexpression of RUNX1 G100V mutant protein.

Depositor

Prashant Mali

Article

Ozturk et al Cell Rep. 2024 Jul 4;43(7):114436. doi:

Insert

RUNX1 (RUNX1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

G100V

Promoter

Ef1A

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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His6-EGFP-PH-PLCD1

Plasmid

#183675

Purpose

PtdIns(4,5)P2 biosensor for purification from bacteria

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

PLCD1 (PLCD1 Human)

Tags

His6-EGFP

Expression

Bacterial

Mutation

Amino acids 11-140

Promoter

T7

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDragon-Ctgf

Plasmid

#155015

Purpose

For Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA, switching to connective tissue growth factor in the presence of Cre and (r)tTA activity.

Depositor

Alberto Rosello-Diez

Article

Ahmadzadeh et al Development. 2020 May 28;147(10). pii: d

Insert

Ccn2 (Ccn2 Mouse)

Use

Mouse Targeting

Mutation

M24T (please see depositor comments)

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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