Addgene

We narrowed to 10,825 results for: cki

Showing: 1741 - 1760 of 10825 results

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pRetroX-PTuner DD-M.EcoGII-v5-Telomeric repeat-binding-factor1

Plasmid

#122084

Purpose

Express M.EcoGII fused to TRF1 with detabilization domain - inducible

Depositor

Laure Crabbe

Article

Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

Insert

DD-linker-M.EcoGII-V5-TERF1

Use

Retroviral

Tags

V5 tag on Nter of TERF1

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_PAX5_PAX5

Plasmid

#205887

Purpose

Express mEGFP-tagged fusion protein, PAX5_PAX5 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

PAX5_PAX5 (PAX5 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pF709-pET28a-Hs-FL-ETFbeta-NHis wt

Plasmid

#85110

Purpose

expression of recombinant human full-length ETFbeta wt with N-terminal 6xHis-tag

Depositor

Pal Falnes

Article

Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

Insert

human full-length ETFbeta (ETFB Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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DHFR-mNeon-GluA1

Plasmid

#173009

Purpose

For light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Intracellular trafficking can be better visualized with mNeon.

Depositor

Matthew Kennedy

Article

Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

DHFR-mNeon-GluA1

Use

Tags

DHFR and mNeon

Expression

Mammalian

Mutation

Promoter

Chicken beta actin (CAG)

Available since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pDK637

Plasmid

#134203

Purpose

bacterial expression of human CENP-N 1-240

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

CENP-N 1-240-His (CENPN Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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YFP-POP5-C1

Plasmid

#134543

Purpose

Expresses YFP-POP5 fusion protein

Depositor

Susan Janicki

Article

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Insert

POP5 (POP5 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pEMS2232

Plasmid

#158401

Purpose

High Copy number plasmid containing Ple316 (NR2E1 MiniPromoter) insert

Depositor

Elizabeth Simpson

Article

Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

Insert

Ple316 (NR2E1 Human)

Use

Unspecified

Tags

Expression

Mutation

Promoter

Ple316

Available since

Feb. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1725

Plasmid

#105870

Purpose

High copy number plasmid containing iCreERT2-FFrt-KanR-FFrt insert

Depositor

Elizabeth Simpson

Article

Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

Insert

iCreERT2-FFrt-KanR-FFrt

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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YFP-Rpp40-C1

Plasmid

#134549

Purpose

Expresses YFP-Rpp40 fusion protein

Depositor

Susan Janicki

Article

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Insert

Rpp40 (RPP40 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Miro2 Δ593-618

Plasmid

#47901

Purpose

Expresses myc tagged Miro2 lacking transmembrane domain

Depositor

Pontus Aspenström

Article

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Insert

Miro2 Δ593-618 (RHOT2 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Δ593-618, lacks transmembrane domain. Also see De…

Promoter

CMV

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pF711-pET28a-Hs-delta25-MCAD-NHis wt

Plasmid

#85112

Purpose

expression of recombinant human mature (delta25) MCAD wt with N-terminal 6xHis-tag

Depositor

Pal Falnes

Article

Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

Insert

human mature (delta25) MCAD (ACADM Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pF714-pET28a-Hs-FL-METTL20-NHis wt

Plasmid

#85115

Purpose

expression of recombinant human full-length METTL20 wt with N-terminal 6xHis-tag

Depositor

Pal Falnes

Article

Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

Insert

human full-length METTL20 (METTL20 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only
pEMS2166

Plasmid

#158412

Purpose

High Copy number plasmid containing Ple331 (PAX6 MiniPromoter) insert

Depositor

Elizabeth Simpson

Article

Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

Insert

Ple331 (PAX6 Human)

Use

Unspecified

Tags

Expression

Mutation

Promoter

Ple331

Available since

Feb. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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YFP-Fibrillarin-C1

Plasmid

#134538

Purpose

Expresses YFP-Fibrillarin fusion protein

Depositor

Susan Janicki

Article

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Insert

Fibrillarin (FBL Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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CFP-Rpp29-C1

Plasmid

#134546

Purpose

Expresses CFP-Rpp29 fusion protein

Depositor

Susan Janicki

Article

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Insert

Rpp29 (POP4 Human)

Use

Tags

CFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-chCARex

Plasmid

#87233

Purpose

bacterial expression of GST-CAR extracellular domain

Depositor

Fritz Rathjen

Article

Patzke et al J Neurosci. 2010 Feb 24;30(8):2897-910.

Insert

CXADR (CXADR Chicken)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pEMS1925

Plasmid

#105869

Purpose

High copy number plasmid containing iCreERT2 synthesized fragment for constructing the HPRT plasmid

Depositor

Elizabeth Simpson

Article

Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

Insert

iCreERT2

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCRISPR-cBEST-v2-SF14P

Plasmid

#209448

Purpose

improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the SF14P promoter

Depositor

Tilmann Weber

Article

Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

Insert

Codon optimized APOBEC1-nCas9-UGI fusion protein

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

PtipA; sgRNA expression from SF14P promoter

Available since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPR-cBEST-v2-kasOP*

Plasmid

#209447

Purpose

improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the kasOP* promoter

Depositor

Tilmann Weber

Article

Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

Insert

Codon optimized APOBEC1-nCas9-UGI fusion protein

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

PtipA; sgRNA expression from kasOP* promoter

Available since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-ALDH1A3gRNA3

Plasmid

#189748

Purpose

The construct was used for expression of gRNA for knocking out of the ALDH1A3 gene in Cas9 expressing cells.

Depositor

Robert Sobol

Article

Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

Insert

ALDH1A3 gRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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