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We narrowed to 6,930 results for: crispr cas9 plasmids

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Showing: 1741 - 1760 of 6930 results

  7. pAAV-CMV-CASANOVA

    Plasmid
    #113036
    Purpose
    AAV vector for CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9
    Depositor
    Dominik Niopek
    Article
    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s
    Insert
    CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
    Use
    AAV, CRISPR, and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Nov. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    113036-plasmid-map-sequence-id-226953

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  9. pEG302 22aa SunTag NtDRMcd (no NLS) g4+g10+g18 (FWA)

    Plasmid
    #117168
    Purpose
    CRISPR Cas9 SunTag system to target NtDRMcd (without an NLS) to the FWA locus with three guide RNAs
    Depositor
    Steven Jacobsen
    Article
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    g18_U6_g10_U6_g4_U6_NOS_NLS_GB1_noNLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available Since
    March 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    117168-plasmid-map-sequence-id-238529

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  10. pUC gRNA Shuttle

    Plasmid
    #47024
    Purpose
    Encodes a template from which gRNAs can be made via InFusion cloning. The Medicago truncatula U6.6 promoter drives the gRNA. For use in plants.
    Depositor
    Wayne Parrott
    Article
    Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:
    Insert
    gRNA Shuttle
    Use
    CRISPR; Cas9
    Expression
    Plant
    Mutation
    G to T cloning mutation at position 323
    Promoter
    Medicago truncatula U6.6
    Available Since
    Sept. 3, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    47024-plasmid-map-sequence-id-67042

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  12. pCY5h

    Plasmid
    #160295
    Purpose
    Yeast CRISPR plasmid targeting the hphMX cassette
    Depositor
    Amir Aharoni
    Article
    Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do
    Inserts
    sgRNA expression cassette (with guide targeting hph: GACCTGATGCAGCTCTCGGA)
    URA3 selection cassette
    sgRNA expression cassette (without guide)
    Cas9
    Use
    CRISPR
    Tags
    8xHis-tag and SV40 NLS
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    160295-plasmid-map-sequence-id-464605

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  13. pCY5n

    Plasmid
    #160296
    Purpose
    Yeast CRISPR plasmid targeting the natMX cassette
    Depositor
    Amir Aharoni
    Article
    Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do
    Inserts
    sgRNA expression cassette (with guide targeting nat: GTACCGCACCAGTGTCCCGG)
    URA3 selection cassette
    sgRNA expression cassette (without guide)
    Cas9
    Use
    CRISPR
    Tags
    8xHis-tag and SV40 NLS
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    160296-plasmid-map-sequence-id-464604

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  14. pCY5k

    Plasmid
    #160294
    Purpose
    Yeast CRISPR plasmid targeting the kanMX cassette
    Depositor
    Amir Aharoni
    Article
    Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do
    Inserts
    sgRNA expression cassette (with guide targeting kan: TGTTTTGCCGGGGATCGCAG)
    URA3 selection cassette
    sgRNA expression cassette (without guide)
    Cas9
    Use
    CRISPR
    Tags
    8xHis-tag and SV40 NLS
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    160294-plasmid-map-sequence-id-464601

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  15. MSP469

    Plasmid
    #65771
    Purpose
    Human expression vector for SpCas9 VQR variant: CMV-T7-humanSpCas9(D1135V/R1335Q/T1337R)-NLS-3xFLAG (VQR variant)
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/R1335Q/T1337R)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135V, R1335Q, and T1337R mutations in Cas9
    Promoter
    CMV & T7
    Available Since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65771-plasmid-map-sequence-id-112653

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  16. MSP680

    Plasmid
    #65772
    Purpose
    Human expression vector for SpCas9 EQR variant: CMV-T7-humanSpCas9(D1135E/R1335Q/T1337R)-NLS-3xFLAG (EQR variant)
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E/R1335Q/T1337R)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135E, R1335Q, and T1337R mutations in Cas9
    Promoter
    CMV & T7
    Available Since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65772-plasmid-map-sequence-id-112654

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  18. pMTF1.1.0-gDNA

    Plasmid
    #113797
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor MTF1
    Depositor
    Kevin White
    Insert
    MTF1 (MTF1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113797-plasmid-map-sequence-id-230615

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  19. pZBTB9.1.0-gDNA

    Plasmid
    #112469
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZBTB9
    Depositor
    Kevin White
    Insert
    ZBTB9 (ZBTB9 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112469-plasmid-map-sequence-id-230499

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  20. pZBTB5.1.0-gDNA

    Plasmid
    #112399
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZBTB5
    Depositor
    Kevin White
    Insert
    ZBTB5 (ZBTB5 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112399-plasmid-map-sequence-id-230250

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Showing: 1741 - 1760 of 6930 results