-
Plasmid#108657PurposeA S6 Kinase biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)DepositorInserthyBRET-S6K-EV
UseTagsXFPExpressionMammalianMutationPromoterpCAGGSAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
Pu2-3::tag::sl1(G16C)
Plasmid#79002PurposeExpression of exogenous SL1 in multiple tissues of C. elegansInsertsls-1.2 (sls-1.2 Nematode)
UseTagsillumina-adapterExpressionWormMutationG16CPromoterF08G2.17Available sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
FKBP_cPPVp
Plasmid#118967PurposeExpresses C-terminus of plum pox virus protease (PPVp) fused to the FK-506 binding protein (FKBP)DepositorInsertsplit cPPVp in fusion with FKBP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
FRB_nPPVp
Plasmid#118966PurposeExpresses N-terminus of plum pox virus protease (PPVp) fused to FKBP-rapamycin binding (FRB) domainDepositorInsertsplit nPPVp in fusion with FRB
UseTagsMyc tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-ER-BSCaM2
Plasmid#64855PurposeEndoplasmic reticulum (ER)-targeted version of a high-affinity FRET-based biosensor for monitoring Ca2+/CaM levels; targeted to cytosolic face of ER membrane.DepositorInsertCYP450-BSCaM2
UseTagsCFP, N-terminal 27 amino acids from CYP450, and Y…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
GOMO-GFP-LUC
Plasmid#60976PurposeRapid selection of recombinant marker-free bioluminescent rodent malaria parasitesDepositorInsertsGFP-Luciferase fusion
mCherry
hDHFR-yFCU
UseTagsExpressionMutationPromoterAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGREAT18
Plasmid#170906PurposeGoldenBraid Double Transcriptional Unit - pAtUBQ10_E-Luc_AtHSP18.2t+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpAtUBQ10Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-ER-CaNARi
Plasmid#64852PurposeEndoplasmic reticulum (ER)-targeted FRET biosensor for monitoring calcineurin (CaN) activation; targeted to cytosolic face of ER membrane.DepositorInsertCYP450-CaNARi
UseTagsCerulean, N-terminal 27 amino acids from CYP450, …ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
Pmyo3::tag:sl1(G16C)
Plasmid#79003PurposeExpression of exogenous SL1 in C. elegans muscleInsertsls-1.2 (sls-1.2 Nematode)
UseTagsillumina-adapterExpressionWormMutationG16CPromotermyo-3Available sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCardinal-CytERM-N-17
Plasmid#56158PurposeLocalization: Endoplasmic Reticulum, Excitation: 604, Emission: 659DepositorInsertCytERM
UseTagsmCardinalExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
GB1-His-SAP25-126-186(pL91)
Plasmid#124387PurposeE.Coli produced SAP25 SID purified by FPLC nickel columnDepositorInsertGB1-His-SAP25-126-186 (SAP25 Human)
UseTagsGB1-HistidineExpressionBacterialMutationPromoterAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGREAT17
Plasmid#170905PurposeGoldenBraid Double Transcriptional Unit - p35SoTMV_E-Luc_AtHSP18.2t+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…Promoterp35SoTMVAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
Pmyo-3::tag:SL1(G20A)
Plasmid#81114PurposeExpression of exogenous SL1(G20A) in C. elegans muscleDepositorInsertsls-1.2 (sls-1.2 Nematode)
UseTagsillumina-adapterExpressionWormMutationG20APromoterpromoter of myo-3Available sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
mIFP-CytERM-N-17
Plasmid#56217PurposeLocalization: Endoplasmic Reticulum, Excitation: 683, Emission: 704DepositorInsertCytERM
UseTagsmIFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 28, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pACUH-GFP1-10 sec
Plasmid#172062PurposeUAS vector to express GFP1-10 in the ER lumenDepositorInsertGFP1-10 sec
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT4
Plasmid#170892PurposeGoldenBraid Double Transcriptional Unit - pG10-90_E-Luc_NOSt+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpG10-90Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRER-PhoCl-Insert
Plasmid#219675PurposeCaging at lumenal N-ter of POI: TMEM-PhoCl-MCS in pTREtight2DepositorInsertRER-PhoCl
UseTagsPhoClExpressionMammalianMutationPromoterTREAvailable sinceJune 14, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
AA416
Plasmid#215992PurposeFragmid fragment: (N' terminus) luminescent reporterDepositorHas ServiceCloning Grade DNAInsertStart; V5_v1.1; NanoLuc_v1.1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBC113
Plasmid#202275PurposeLevel 2 for strain-specific barcode DNA tag with bc140 through Tn7 bacterial insertion, Tetracyline selectable marker and far-red-FP fluorescent marker mPlumDepositorInsertbc140
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBC115
Plasmid#202277PurposeLevel 2 for strain-specific barcode DNA tag with bc142 through Tn7 bacterial insertion, Tetracyline selectable marker and far-red-FP fluorescent marker mPlumDepositorInsertbc142
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBC108
Plasmid#202247PurposeLevel 2 for strain-specific barcode DNA tag with bc135 through Tn7 bacterial insertion, Gentamicin selectable marker and far-red-FP fluorescent marker mPlumDepositorInsertbc135
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBC130
Plasmid#202257PurposeLevel 2 for strain-specific barcode DNA tag with bc157 through Tn7 bacterial insertion, Kanamycin selectable marker and far-red-FP fluorescent marker mPlumDepositorInsertbc157
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBC131
Plasmid#202258PurposeLevel 2 for strain-specific barcode DNA tag with bc158 through Tn7 bacterial insertion, Kanamycin selectable marker and far-red-FP fluorescent marker mPlumDepositorInsertbc158
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBC104
Plasmid#202243PurposeLevel 2 for strain-specific barcode DNA tag with bc131 through Tn7 bacterial insertion, Gentamicin selectable marker and far-red-FP fluorescent marker mPlumDepositorInsertbc131
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBC105
Plasmid#202244PurposeLevel 2 for strain-specific barcode DNA tag with bc132 through Tn7 bacterial insertion, Gentamicin selectable marker and far-red-FP fluorescent marker mPlumDepositorInsertbc132
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBC106
Plasmid#202245PurposeLevel 2 for strain-specific barcode DNA tag with bc133 through Tn7 bacterial insertion, Gentamicin selectable marker and far-red-FP fluorescent marker mPlumDepositorInsertbc133
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBC107
Plasmid#202246PurposeLevel 2 for strain-specific barcode DNA tag with bc134 through Tn7 bacterial insertion, Gentamicin selectable marker and far-red-FP fluorescent marker mPlumDepositorInsertbc134
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-mMOV10-3'UTR-MRE16+MRE153-mut
Plasmid#178910Purposedual luminescence reporter to assess miRNA responsiveness in the 3'UTR of mouse Mov10 RNA helicaseDepositorInsert3'UTR mMov10 (Mov10 Mouse)
UseLuciferaseTagsExpressionMutationCTG>AGT and TGC>CAT mir16+153 mutatedPromoterSV40 promoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-mMOV10-3'UTR-MRE16-mut1
Plasmid#178906Purposedual luminescence reporter to assess miRNA responsiveness in the 3'UTR of mouse Mov10 RNA helicaseDepositorInsert3'UTR mMov10 (Mov10 Mouse)
UseLuciferaseTagsExpressionMutationCTG>AGT miR16-5p MRE1 mutatedPromoterSV40 promoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-mMOV10-3'UTR-MRE153-mut4
Plasmid#178909Purposedual luminescence reporter to assess miRNA responsiveness in the 3'UTR of mouse Mov10 RNA helicaseDepositorInsert3'UTR mMov10 (Mov10 Mouse)
UseLuciferaseTagsExpressionMutationTGC>CAT miR153-3p MRE mutatedPromoterSV40 promoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGREAT11
Plasmid#170899PurposeGoldenBraid Double Transcriptional Unit - pNOS_E-Luc_AtACT2t+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpNOSAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT15
Plasmid#170903PurposeGoldenBraid Double Transcriptional Unit - pCmYLCV911_E-Luc_AtACT2t+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpCmYLCV911Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT16
Plasmid#170904PurposeGoldenBraid Double Transcriptional Unit - pNOS_E-Luc_AtHSP18.2t+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpNOSAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT19
Plasmid#170907PurposeGoldenBraid Double Transcriptional Unit - pG10-90_E-Luc_AtHSP18.2t+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpG10-90Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT20
Plasmid#170908PurposeGoldenBraid Double Transcriptional Unit - pCmYLCV911_E-Luc_AtHSP18.2t+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpCmYLCV911Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT21
Plasmid#170909PurposeGoldenBraid Double Transcriptional Unit - pNOS_E-Luc_35St+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpNOSAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT22
Plasmid#170910PurposeGoldenBraid Double Transcriptional Unit - p35SoTMV_E-Luc_35St+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…Promoterp35SoTMVAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT23
Plasmid#170911PurposeGoldenBraid Double Transcriptional Unit - pAtUBQ10_E-Luc_35St+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpAtUBQ10Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT24
Plasmid#170912PurposeGoldenBraid Double Transcriptional Unit - pG10-90_E-Luc_35St+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpG10-90Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT25
Plasmid#170913PurposeGoldenBraid Double Transcriptional Unit - pCmYLCV911_E-Luc_35St+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpCmYLCV911Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT27
Plasmid#170915PurposeGoldenBraid Double Transcriptional Unit - pAtNR1_E-Luc_AtHSP18.2t+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpAtNR1Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT3
Plasmid#170891PurposeGoldenBraid Double Transcriptional Unit - pAtUBQ10_E-Luc_NOSt+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpAtUBQ10Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT5
Plasmid#170893PurposeGoldenBraid Double Transcriptional Unit - pCmYLCV911_E-Luc_NOSt+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpCmYLCV911Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT6
Plasmid#170894PurposeGoldenBraid Double Transcriptional Unit - pNOS_E-Luc_NtEUt+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpNOSAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT7
Plasmid#170895PurposeGoldenBraid Double Transcriptional Unit - p35SoTMV_E-Luc_NtEUt+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…Promoterp35SoTMVAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT8
Plasmid#170896PurposeGoldenBraid Double Transcriptional Unit - pAtUBQ10_E-Luc_NtEUt+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpAtUBQ10Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT9
Plasmid#170897PurposeGoldenBraid Double Transcriptional Unit - pG10-90_E-Luc_NtEUt+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpG10-90Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT10
Plasmid#170898PurposeGoldenBraid Double Transcriptional Unit - pCmYLCV911_E-Luc_NtEUt+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpCmYLCV911Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT12
Plasmid#170900PurposeGoldenBraid Double Transcriptional Unit - p35SoTMV_E-Luc_AtACT2t+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…Promoterp35SoTMVAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT13
Plasmid#170901PurposeGoldenBraid Double Transcriptional Unit - pAtUBQ10_E-Luc_AtACT2t+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpAtUBQ10Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only