We narrowed to 18,716 results for: IRE

1. pDisplay-GRAB-rDA3h-IRES-EGFP-CAAX

   Plasmid

   #208697

   Purpose

   Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3h and a membrane-localized EGFP in mammalian cells

   Depositor

   Yulong Li

   Article

   Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   GPCR activation based dopamine (DA) sensor GRAB_rDA3h
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CBh-mKate2-IRES-MCS (C-gap-WT)

   Plasmid

   #105922

   Purpose

   AAV-mediated gene expression from CBh promoter. Contains left AAV2 ITR with an 11bp deletion in C region and canonical right AAV2 ITR

   Depositor

   Marcella Patrick

   Inserts

   mKate2
   IRES-Multiple cloning site
   

   Use

   AAV

   Tags

   Expression

   Mutation

   codon optimized

   Promoter

   CBh and same CBh promoter as mKate2

   Available since

   Feb. 15, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pHAGE-EF1a MICA*009-IRES-ZsGreen

   Plasmid

   #114007

   Purpose

   Induces expression of human MICA allele 009 cDNA

   Depositor

   Kai Wucherpfennig

   Article

   Ferrari de Andrade et al Science. 2018 Mar 30;359(6383):1537-1542

   Insert

   MICA*009 (MICA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-EF1a-EGFP-VAMP2-IRES-Puromycin

   Plasmid

   #134661

   Purpose

   Expresses EGFP-tagged synaptic vesicle protein

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   vesicle-associated membrane protein 2 (VAMP2 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVX-EF1a-EGFP-Membrin-IRES-Puromycin

   Plasmid

   #134865

   Purpose

   Expresses EGFP-tagged Golgi apparatus marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Membrin (GOSR2 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-Flag-CDK19-W105M-IRES-ZsGreen

   Plasmid

   #68859

   Purpose

   Expresses Human CDK19 W105M Mutant

   Depositor

   Matthew Shair

   Article

   Pelish et al Nature. 2015 Sep 28. doi: 10.1038/nature

   Insert

   Cyclin-Dependent Kinase 19 (CDK19 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   W105M

   Promoter

   EF1α

   Available since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSCV-EZH2-PGK-Puro-IRES-GFP

   Plasmid

   #75125

   Purpose

   Retroviral expression plasmid encoding wild type human EZH2

   Depositor

   Christopher Vakoc

   Article

   Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

   Insert

   KMT6 (EZH2 Human)
   

   Use

   Retroviral

   Tags

   None

   Expression

   Mutation

   Promoter

   Available since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pLV UBC APEX2-3xFLAG-IRES-eGFP

   Plasmid

   #214983

   Purpose

   Cytosolic APEX2 control. Can be used as a C-terminal APEX2 fusion by digestion with NheI and Gibson cloning (see comments).

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   APEX2 (APX2 Glycine max (soybean))
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   UBC

   Available since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pB-CAG-B19L-IRES-mCherry-WPRE-BGHpA

   Plasmid

   #178518

   Purpose

   For making Rabies virus SAD B19 strain L cell line with hyPBase

   Depositor

   Ian Wickersham

   Article

   Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

   Insert

   B19L-IRES-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMXS-IRES-BLAST flag-CAD S1859A

    Plasmid

    #169821

    Purpose

    Expresses C-terminal flag-tagged CAD with mutation of reported S6K phosphorylation site

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    S1859A; TCCC -> AGTC silent mutations at nt527…

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-Flag-CDK8-W105M-IRES-mCherry

    Plasmid

    #68857

    Purpose

    Expresses Human CDK8 W105M Mutant

    Depositor

    Matthew Shair

    Article

    Pelish et al Nature. 2015 Sep 28. doi: 10.1038/nature

    Insert

    Cyclin-Dependent Kinase 8 (CDK8 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    W105M

    Promoter

    EF1α

    Available since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. CAG-DDdCas9VP192-T2A-EGFP-ires-puro

    Plasmid

    #69534

    Purpose

    DHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim.

    Depositor

    Timo Otonkoski

    Article

    Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

    Insert

    DD-dCas9VP192-T2A-EGFP
    

    Use

    Tags

    C-term T2A-EGFP and DHFR Destabilised Domain

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. p3E-IRES-3xNLS-mScarlet-I3-pA (JDW 1360)

    Plasmid

    #224536

    Purpose

    A Gateway compatible 3' entry clone containing IRES-FLAG- NLS-mScarlet-I3

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    IRES-FLAG- NLS-mScarlet-I3
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. MSCV-human CSF-1Rwt-IRES-GFP

    Plasmid

    #115523

    Purpose

    Mammalian expression construct

    Depositor

    Martine Roussel

    Article

    Roussel et al Nature. 1987 Feb 5-11;325(6104):549-52.

    Insert

    human CSF-1 receptor (CSF1R Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV SYN PSAM4 GlyR IRES EGFP

    Plasmid

    #119742

    Purpose

    Chemogenetic inhibitor expression plasmid

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2019 Mar 14. pii: science.aav52

    Has Service

    AAV5

    Insert

    PSAM4 GlyR IRES eGFP (CHRNA7 Synthetic, Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    L131G, Q139L, Y217F

    Promoter

    synapsin

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. SARS-CoV-2 Nucleocapsid-3xHA-IRES-mcherry

    Plasmid

    #173030

    Purpose

    To express SARS-CoV-2 Nucleocapsid protein tagged with 3xHA and in frame with mcherry (NCP-IRES-mcherry) in mammalian cells

    Depositor

    Mohamed Fareh

    Article

    Fareh et al Nat Commun. 2021 Jul 13;12(1):4270. doi:

    Insert

    SARS-CoV-2 Nucleocapsid phosphoprotein
    

    Use

    Lentiviral

    Tags

    3xHA and IRES-mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    Gag (truncated)

    Available since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMSCV-IRES-GFP/CALR del52-FLAG

    Plasmid

    #204523

    Purpose

    Retroviral expression of human CALR del52-FLAG

    Depositor

    Norio Komatsu

    Article

    Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

    Insert

    CALR del52-FLAG (CALR Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    del52

    Promoter

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-CAG-NOTCH2NLB (N2NL-FL)-ires-mCherry

    Plasmid

    #122957

    Purpose

    Lentiviral expression of NOTCH2NLB full length under CAG promoter with mCherry expression

    Depositor

    Pierre Vanderhaeghen

    Article

    Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

    Insert

    NOTCH2NLB (NOTCH2NLB Human)
    

    Use

    Lentiviral

    Tags

    6xmyc tag

    Expression

    Mutation

    Promoter

    CAG promoter

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-EF1a-MinE(L4E)-EGFP_IRES-Blast

    Plasmid

    #213117

    Purpose

    Expression of a faster-running MinE mutant in mammalian cells

    Depositor

    Scott Coyle

    Article

    Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

    Insert

    MinE(L4E)-EGFP (minE Escherichia coli)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    L4E

    Promoter

    EF1a

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSyn-DIO-HA-rM3D(Gs)-IRES-mCitrine

    Plasmid

    #50456

    Purpose

    Double floxed Gs-coupled rM3D-IRES-mCitrine under the control of human synapsin promoter

    Depositor

    Bryan Roth

    Insert

    rM3D-IRES-mCitrine (CHRM3 Human)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    human Synapsin 1

    Available since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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