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Plasmid#216548PurposeFor expression of N-flag tagged ZoAl-ENT R2 protein in mammalian cellsDepositorInsertR2 retroelement protein
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEP163
Plasmid#137881PurposePiggyBac donor vector for expression of mRFP-T2A-BlasticidinRDepositorInsertmRFP-T2A-BlasticidinR
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH7m34GW-35S:SNACS
Plasmid#203576PurposePlant SnRK2 kinase activity FRET sensor in plant expression vectorDepositorInsertSNACS
UseTagsStrepIIExpressionPlantMutationnonePromoterAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLS.492
Plasmid#184957PurposeTest effect of a1/a2 length on editing rpoB using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 22
UseTagsExpressionBacterialMutationrpoB donor T1534C, a1/a2 length extended to 22 bpPromoterT7/lacAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
Dimer cleaved
Plasmid#198199PurposeFRET calibration: Dimer construct consisting of mTurquoise2 and mVenus with a 2A peptide linker for posttranslational linker cleavageDepositorInsertmVenus-T2A-mTurquoise2
UseTagsExpressionMammalianMutationA206K bothPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHRE
Plasmid#134909PurposeOverexpression of recombinant proteins in plants thanks to efficient translational efficiency. Empty vector, clone ORF using Bsa1 sites. Test UTR combinations by swapping 5' and 3' UTRs.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationPromoterdouble 35SAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsAGO1_F
Plasmid#146314PurposeMammalian Expression of HsAGO1DepositorInsertHsAGO1 (AGO1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
mNeonGreen-DEVD-NanoLuc
Plasmid#98287Purposecaspase-3 BRET SensorDepositorInsertmNeonGreen-LEHD-NanoLuc
UseTagsHis-tagExpressionMammalianMutationPromoterCMVAvailable sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
ncvB-ssGrl1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
Plasmid#140168PurposeExpresses a regulatable secretory cargo for Tetrahymena thermophilaDepositorInsertssGrl1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
UseOtherTagsExpressionMutationPromoterMTT1Available sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLSSmOrange-mKate2
Plasmid#99868PurposeMeasure FRET between LSSmOrange and mKate2DepositorInsertLSSmOrange
UseTagsmKate2ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
L1_UBE2:mS-Lt-Ck3
Plasmid#136131PurposeL1 in position 3, mark cell outline with mScarletIDepositorInsertp5-MpUBE2:mScarletI-Lti6b_3tNos-35S
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-mem-TurboID
Plasmid#149409PurposeMammalian expression of secretory pathway membrane-localized TurboID, N-term HA tag, C-term V5 tag, in mammalian expression plasmid. Amp/Neo selectionDepositorInsertmem-TurboID
UseTagsHA and V5ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMT525
Plasmid#131392PurposeL1RP retrotransposition reporterDepositorInsertL1RP GFP-AI
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
L1_CsR-Ck1
Plasmid#136124PurposeL1 in position 1 (next to LB in L2 construct), for CsRDepositorInsertp5-35Sx2:mALS_3t35S
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUAP4
Plasmid#136079PurposeAcceptor plasmid for L0 parts. Clone L0 parts into pUAP4 with SapI, lacZ blue-white screening. Release parts to assemble transcription units with BsaI.DepositorInsert
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCk4_spacer
Plasmid#136074PurposeUse when making even level constructs (L2,...) if pCk4 position (position 4) has no construct assignedDepositorInsertOdd spacer
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
3TERM_Nos-35S
Plasmid#136116Purpose3TERM after CDS/ CDS12-CTAGDepositorInsert3TERM_Nos-35S (A. tumefaciens, CaMV)
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCk3_spacer
Plasmid#136073PurposeUse when making even level constructs (L2,...) if pCk3 position (position 3) has no construct assignedDepositorInsertOdd spacer
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
PROM5_MpEF1a
Plasmid#136113PurposeStrong promoter from MpEF1a, that drives expression everywhere in Mp gemmae but stronger in the notchesDepositorInsertPROM5_MpEF1a Mapoly0024s0116
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCk2
Plasmid#136696PurposeAcceptor plasmid for odd level position 2, lacZ blue-white screening. Part of the OpenPlant toolkit.DepositorInsertlacZ
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only