We narrowed to 858 results for: GI
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Plasmid#207579PurposeHomologous recombination donor replacing TR with a puro cassette.DepositorInsertpuro resistance cassette flanked by TR locus sequences excluding TR
TagsNoneExpressionMammalianPromoterSV40Available SinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
sgRNA TR KO 1
Plasmid#207609PurposesgRNA 1 to knockout TR by replacement with a PuroR cassetteDepositorInsertACCCTAACTGAGAAGGGCGT
TagsNoneExpressionMammalianPromoterCMV and U6Available SinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2 Neo
Plasmid#127644PurposeEmpty pLentiCRISPRv2 with NeoRDepositorTypeEmpty backboneUseLentiviralAvailable SinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
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mCherry-Dyskerin
Plasmid#207577PurposeN-terminal mCherry-tag using SalI/EcoRIDepositorInsertgenerated by restriction cloning of gene into a modified pBABE-puro vector containing the N-terminal mCherrytag using SalI/EcoRI
TagsmCherryExpressionMammalianPromoterLTRAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-Halo-Dyskerin
Plasmid#207578PurposeCRISPR-Cas9 genome editing encoding a FLAG-HaloTag at the endogenous dyskerin locusDepositorInsertHaloTag DKC1
TagsHaloTagExpressionMammalianPromoterCMVAvailable SinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
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Halo-TCAB1 HRD
Plasmid#207535PurposeHomologous recombination donor to insert HaloTag at the N-terminus of the endogenous TCAB1 locus.DepositorInsertHaloTag with internal PuroR cassette flanked by human TCAB1 locus sequences
TagsHaloTagExpressionMammalianPromoterEndogenousAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pET22b_EcSSB_2
Plasmid#78204PurposeExpression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensorDepositorInsertssb (W88C)
ExpressionBacterialMutationW88C (please see depositor comment below)PromoterT7Available SinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 RL3Sc
Plasmid#109365PurposeHuman rod opsin chimera with intracellular loop 3 from Scallop opsin with 1D4 tagDepositorInsertTags1D4ExpressionMammalianPromoterCMVAvailable SinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 RL3Am
Plasmid#109364PurposeHuman rod opsin chimera with intracellular loop 3 from Amphioxus opsin 1 with 1D4 tagDepositorInsertRod opsin chimera with Amphioxus Opsin1 intracellular Loop 3 (RHO Branchiostoma belcheri, Human)
Tags1D4ExpressionMammalianPromoterCMVAvailable SinceMay 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
3xMS-TR-100-PURO HRD
Plasmid#207582Purposehomologous recombination donor for the insertion of the 3xMS2-tag at the endogenous locusDepositorInsert3xMS2-TR and 100 bp of downstream sequence followed by a PuroR and flanked by endogenous TR sequences
Tags3xMS2ExpressionMammalianPromoterSV40Available SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
3xMS2 TR knockin sgRNA 1
Plasmid#207607PurposesgRNA for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locusDepositorInsertcgactcgcccggcagcgcac
TagsNoneExpressionMammalianPromoterCMV and U6Available SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
3xMS2 TR knockin sgRNA 2
Plasmid#207608PurposesgRNA 2 for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locusDepositorInsertacccccaaacctgactgact
TagsNoneExpressionMammalianPromoterCMV and U6Available SinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-Tapasin delta2step
Plasmid#153479PurposeMammalian expression of FLAG-tagged Tapasin delta2stepDepositorInsertTAPBP (TAPBP Human)
TagsFLAGExpressionMammalianMutationdeletion of aa.13-14, 16-17PromoterCMVAvailable SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-Tapasin delta3step
Plasmid#153480PurposeMammalian expression of FLAG-tagged Tapasin delta3stepDepositorInsertTAPBP (TAPBP Human)
TagsFLAGExpressionMammalianMutationdeletion of aa.12-14, 16-18PromoterCMVAvailable SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-Tapasin G15E
Plasmid#153481PurposeMammalian expression of FLAG-tagged Tapasin G15EDepositorAvailable SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-Tapasin G15L
Plasmid#153482PurposeMammalian expression of FLAG-tagged Tapasin G15LDepositorAvailable SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-Tapasin L18E
Plasmid#153483PurposeMammalian expression of FLAG-tagged Tapasin L18EDepositorAvailable SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only