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Plasmid#159936PurposeOpto-MB bacterial expressionDepositorInsertanti-SH2 MB with SS58 insert
UseTagsExpressionBacterialMutationAsLOV2 V416LPromoterAvailable sinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCri_L889-antiSH2-OptoMB-Triple
Plasmid#159937PurposeOpto-MB bacterial expressionDepositorInsertanti-SH2 MB with SS58 insert
UseTagsExpressionBacterialMutationAsLOV2 V416I_G528A_N538EPromoterAvailable sinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
LaM8-AK74
Plasmid#159594PurposeOpto-NanobodyDepositorInsertLaM8 with AsLOV2 at AK74
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
LaM8-GG15-irFP
Plasmid#159591PurposeOpto-NanobodyDepositorInsertLaM8 with AsLOV2 at GG15
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQP-CiRISB
Plasmid#138978PurposeLight-controlled tri-directional targeting of endogenous proteins (cytoplasm-shifted)DepositorInsertNcoI-BphP1-CAAX-IRES2-iB(GFP)-NESmCherry- Q-PAS1-AsLOV2cNLS-NotI
UseTagsExpressionMutationPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
LaM8-GG15
Plasmid#159593PurposeOpto-NanobodyDepositorInsertLaM8 with AsLOV2 at GG15
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTrc_AraC-I113-LOV
Plasmid#206804PurposeExpresses AraC-I113-LOV from a Trc promoter. For use in E. coliDepositorInsertAraC-AsLOV2 fusion
UseSynthetic BiologyTagsHis-tagExpressionBacterialMutationPromoterAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTrc_AraC-S170-LOV
Plasmid#206805PurposeExpresses AraC-S170-LOV from a Trc promoter. For use in E. coliDepositorInsertAraC-AsLOV2 fusion
UseSynthetic BiologyTagsHis-tagExpressionBacterialMutationPromoterAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQP-NiRISB
Plasmid#138977PurposeLight-controlled tri-directional targeting of endogenous proteins (nucleus-shifted)DepositorInsertNcoI-BphP1-CAAX-IRES2-NES- iB(GFP)- mCherry-Q-PAS1-AsLOV2cNLS-NotI
UseTagsExpressionMutationPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQP-iRISB
Plasmid#138979PurposeLight-controlled tri-directional targeting of endogenous proteinsDepositorInsertNcoI-BphP1-CAAX-IRES2-NES-SGlinkeriB( GFP)-mCherry-Q-PAS1-AsLOV2cNLS-NotI
UseTagsExpressionMutationPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSFFV_Gal4LOV[SK22]-VP64-IRES-mCherry (pLZA140)
Plasmid#203907PurposeEncodes LightsOut (AsLOV2(408-543) inserted in Gal4 SK22) transcription factor in mammalian cellsDepositorInsertGal4LOV(SK22)-VP64-IRES-mCherry
UseTagsExpressionMammalianMutationPromoterSFFVAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF-1α_ZF9LOV[C2]-VP64 (pLZA126)
Plasmid#203911PurposeEncodes engineered opto-ZF9 with AsLOV2(408-543) inserted in C2 position in mammalian cellsDepositorInsertZF9LOV[C2]-VP64
UseTagsExpressionMammalianMutationPromoterEF1αAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF-1α_ZF9LOV[C1]-VP64 (pLZA125)
Plasmid#203910PurposeEncodes engineered opto-ZF9 with AsLOV2(408-543) inserted in C1 position in mammalian cellsDepositorInsertZF9LOV[C1]-VP64
UseTagsExpressionMammalianMutationPromoterEF1αAvailable sinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
PAC_E83_LOV_eGFP
Plasmid#231574PurposeThis plasmid mediates puromycin resistance in the dark, resulting in puro mediated cell death upon illumination with blue lightDepositorInsertPAC-P2A-GFP carrying an insertion of AsLOV2 behind E83
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDN77
Plasmid#61347PurposemCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/IkBα NES)DepositorInsertmCherry
UseTagsGS linker-AsLov2-biNLS2 and NES IkBaExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDN34
Plasmid#61343PurposemCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/PKIt NES)DepositorInsertmCherry
UseTagsGS linker-AsLov2-biNLS2 and PKIt NESExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDN80
Plasmid#61349PurposeLINuS-flex: modular LINuS construct enabling introduction of any protein encoding sequence, NES and NLS. The sequence of interest replaces the LexA DBD-MBP-VP64 fragment.DepositorTypeEmpty backboneUseTagsAsLOV2 wt and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDB22
Plasmid#61342PurposeMammalian expression of mCherry fused to LINuS, a light-inducible nuclear localization signal (cMycP1A NLS/PKIt NES)DepositorInsertmCherry
UseTagsGS linker-AsLov2-cMyc1A NLS and PKIt NESExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDN42
Plasmid#61345PurposemCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS10/PKIt NES)DepositorInsertmCherry
UseTagsGS linker-AsLov2-biNLS10 and PKIt NESExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDN41
Plasmid#61344PurposemCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS9/PKIt NES)DepositorInsertmCherry
UseTagsGS linker-AsLov2-biNLS9 and PKIt NESExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only