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Plasmid#8474DepositorInsertRaptor (RPTOR Human)
UseTagshaExpressionMammalianMutation391SQ392-PA (S391P, Q392A)PromoterAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
mutpRL-TK 3xUTR
Plasmid#40758PurposeTo evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNADepositorInsert3 copies of the CXCR4 small RNA target site
UseLuciferaseTagsluciferaseExpressionMutationmutated from TAG to CTC at nt 387–389 of the Reni…PromoterTKAvailable sinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX-cIAP2mut
Plasmid#8336DepositorInsertciap2mut (BIRC3 Human)
UseTagsGSTExpressionBacterialMutationH574APromoterAvailable sinceNov. 15, 2005AvailabilityAcademic Institutions and Nonprofits only -
HA Raptor mutant 7
Plasmid#8475DepositorInsertRaptor (RPTOR Human)
UseTagshaExpressionMammalianMutation738SLQN741-PAAA (S738P, L739A, Q740A, N741A)PromoterAvailable sinceMay 11, 2005AvailabilityAcademic Institutions and Nonprofits only -
pTRETightBI-RY-HMGA2mutant
Plasmid#31470DepositorInserteYFP and mCherry
UseTagsNLSExpressionMammalianMutationPromoterAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
BcatMut-Tyr489
Plasmid#24197DepositorInsertbeta-catenin (CTNNB1 Human)
UseTagsExpressionMammalianMutationY489FPromoterAvailable sinceMarch 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
GFP-MuTMC2truncated
Plasmid#154909PurposeFor the expression and purification of the truncated TMC2 protein from Melopsittacus undulates. The protease cleavage site was replaced with the HRV 3C site.DepositorInsertGFP-MuTMC2truncated
UseTags8histidine-EGFPExpressionInsectMutationTruncations of the residues 1-179 and 691-716PromoterAvailable sinceJuly 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(RBSmut)
Plasmid#149574Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30Available sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
HA Raptor mutant 2
Plasmid#8471DepositorInsertRaptor (RPTOR Human)
UseTagshaExpressionMammalianMutation261DLF263-AAA (D261A, L262A, F263A)PromoterAvailable sinceMay 11, 2005AvailabilityAcademic Institutions and Nonprofits only -
pEx_Hsp70::attBmut
Plasmid#48878PurposePhiC31 exchange vector with the Hsp70 promoter driving tagRFPt from the landing site. Template for enhancer tests.DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceDec. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
HA Raptor mutant 3
Plasmid#8473DepositorInsertRaptor (RPTOR Human)
UseTagshaExpressionMammalianMutation313NWIF316-AAAA (N313A, W314A, I315A, F316A)PromoterAvailable sinceMay 11, 2005AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1P MUTYH_2
Plasmid#160786PurposeSuppress MUTYHDepositorInsertshMUTYH_2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1P MUTYH_1
Plasmid#160785PurposeSuppress MUTYHDepositorInsertshMUTYH_1
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMCGL-T7Mutant
Plasmid#32245DepositorInsertGlucosamine-6-phosphate activated ribozyme
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
circRAJ31-Toehold_mutant
Plasmid#69930PurposeExpresses a toehold mutant of the circRAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. This mutant does not activate cisRAJ31.DepositorInsertplTet-circRAJ31_toeholdmutant
UseSynthetic BiologyTagsExpressionMutation4 bases mutated in toehold of riboregulatorPromoterPltetO1-2Available sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
BcatMut-Tyr604
Plasmid#24196DepositorInsertbeta-catenin (CTNNB1 Human)
UseTagsExpressionMammalianMutationY604FPromoterAvailable sinceMarch 9, 2010AvailabilityAcademic Institutions and Nonprofits only -
BcatMut-Tyr670
Plasmid#24198DepositorInsertbeta-catenin (CTNNB1 Human)
UseTagsExpressionMammalianMutationY670FPromoterAvailable sinceMarch 9, 2010AvailabilityAcademic Institutions and Nonprofits only -
BcatMut-Tyr432
Plasmid#24200DepositorInsertbeta-catenin (CTNNB1 Human)
UseTagsExpressionMammalianMutationY432FPromoterAvailable sinceMarch 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRL-TK 4x mut G
Plasmid#11320DepositorInsert4 bulged binding sites for CXCR4 siRNA antisense
UseLuciferaseTagsRr-lucExpressionMutationMutation G (see paper) on inner 2 binding sites.PromoterAvailable sinceFeb. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGEX-XIAPmut
Plasmid#8341DepositorInsertXIAPmut (XIAP Human)
UseTagsGSTExpressionBacterialMutationH467APromoterAvailable sinceNov. 15, 2005AvailabilityAcademic Institutions and Nonprofits only