Addgene

We narrowed to 13,793 results for: NTS

Showing: 161 - 180 of 13793 results

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pEXP5-NT-5th R

Plasmid

#42572

Depositor

Ivan Korendovych

Article

Moroz et al Angew Chem Int Ed Engl. 2013 Apr 29. doi

Insert

mutated calmodulin gene

Use

Tags

Polyhistidine (6×His) region, TEV recognition sit…

Expression

Bacterial

Mutation

changed Phenylalanine 92 to Glutamine acid, Isole…

Promoter

T7

Available since

March 4, 2013

Availability

Academic Institutions and Nonprofits only

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pEXP5-NT-7th R

Plasmid

#42573

Depositor

Ivan Korendovych

Article

Moroz et al Angew Chem Int Ed Engl. 2013 Apr 29. doi

Insert

mutated calmodulin gene

Use

Tags

Polyhistidine (6×His) region, TEV recognition sit…

Expression

Bacterial

Mutation

changed Phenylalanine 92 to Glutamine acid, Isole…

Promoter

T7

Available since

March 4, 2013

Availability

Academic Institutions and Nonprofits only

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Sars Cov 2 MPRO R188S mutantSARS-CoV-2 Mpro protease (R188S mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204785

Purpose

Bacterial expression of Sars Cov 2 MPRO R188S mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Use

Tags

Expression

Bacterial

Mutation

R188S

Promoter

Available since

Jan. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLKO.1 Blast NT shRNA

Plasmid

#123306

Purpose

Expression of a non-targeting shRNA

Depositor

Giovanni Tonon

Article

Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

Insert

Non-targeting shRNA

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(NT)-PGKpuro2ABFP-W

Plasmid

#163169

Purpose

Lentiviral plasmid with non-targeting gRNA, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

Insert

Non-targeting

Use

CRISPR and Lentiviral

Tags

BFP

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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PCLP-2/NT-IgG (104)

Plasmid

#24423

Depositor

Steven Rosen

Article

Sassetti et al J Biol Chem. 2000 Mar 24. 275(12):9001-1

Insert

PCLP-2 (PODXL2 Human)

Use

Tags

Human IgG1 Fc

Expression

Mammalian

Mutation

N-terminal (acidic) domain of PCLP-2 (1-630 bp) f…

Promoter

Available since

Sept. 27, 2010

Availability

Academic Institutions and Nonprofits only
pM148: pAAV.CMV-Cas13e-NT sgRNA

Plasmid

#203446

Purpose

Plasmid expressing active RfxCas13d with non-targeting gRNA

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Inserts

U6-non targeting (NT) sgRNA
Cas13e

Use

AAV

Tags

HA and NLS

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2702-CMV-iNme2-ABE8e-nt

Plasmid

#228723

Purpose

Expresses N-terminal ABE8e-iNme2 nCas9 (D16A) base editor in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

N-terminal ABE8e with iNme2 nCas9 (D16A)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Nme2-Cas9 D16A D844G E868K K870R D873A D911G K929…

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2703-CMV-iNme2Smu-ABE8e-nt

Plasmid

#228724

Purpose

Expresses N-terminal ABE8e-iNme2Smu nCas9 (D16A) base editor in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

N-terminal ABE8e with iNme2Smu nCas9 (D16A)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Nme2-Cas9, Smu PID, D16A D844G E868K K870R D873A …

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_His6-GST-TEV

Plasmid

#223878

Purpose

4G-cloning donor for N-terminal His6-GST-TEV tag

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

His6-GST-TEV

Use

Golden-gate donor

Tags

Expression

Mutation

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_His6-MBP-TEV

Plasmid

#223877

Purpose

4G-cloning donor for N-terminal His6-MBP-TEV tag

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

His6-MBP-TEV

Use

Golden-gate donor

Tags

Expression

Mutation

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_His6-HA2-TEV

Plasmid

#223876

Purpose

4G-cloning donor for N-terminal His6-HA2-TEV tag

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

His6-HA2-TEV

Use

Golden-gate donor

Tags

Expression

Mutation

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_His6-FLAG3-TEV

Plasmid

#223875

Purpose

4G-cloning donor for N-terminal His6-FLAG3-TEV tag

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

His6-FLAG3-TEV

Use

Golden-gate donor

Tags

Expression

Mutation

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_His10-TS-3C

Plasmid

#223884

Purpose

4G-cloning donor for N-terminal His10-TwinStrep-3C tag

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

His10-TwinStrep-3C

Use

Golden-gate donor

Tags

Expression

Mutation

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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mU6-CR1-CRISPRiv2_hU6-CR3-NT-ctrl-guide_EF1a-PuroR-T2A-BFP

Pooled Library

#197348

Purpose

To allow for genome-wide genetic modifier screening, the library has a second fixed non-targeting control guide.

Depositor

Rebecca Voorhees, Jonathan Weissman

Article

Guna et al BMC Genomics. 2023 Oct 30;24(1):651. doi

Species

Homo sapiens

Use

Lentiviral

Available since

May 25, 2023

Availability

Academic Institutions and Nonprofits only
pEXP5-NT/6xHis mCherry F30 2xdBroccoli

Plasmid

#169233

Purpose

Constitutive T7 RNAP-mediated expression of 6xHis-mCherry with 2xd Broccoli RNA aptamer.

Depositor

Dora Tang

Article

Gonzales et al ACS Synth Biol. 2022 Jan 21;11(1):205-21

Insert

6xHis mCherry F30-2xdBroccoli

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Dec. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pSET-dCas9-actII-4-NT-S1

Plasmid

#110185

Purpose

Repression of gene expression in Streptomyces by CRISPRi

Depositor

Yinhua Lu

Article

Zhao et al Biotechnol J. 2018 Jun 3. doi: 10.1002/b

Inserts

dCas9
sgRNA

Use

CRISPR; I

Tags

Expression

Bacterial

Mutation

D10A, H840A

Promoter

J23119 and ermE*p

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pEJS2701-CMV-Nme2Smu-ABE8e-nt-E932R

Plasmid

#228722

Purpose

Expresses N-terminal ABE8e-Nme2Smu nCas9 (D16A) base editor with E932R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

N-terminal ABE8e with Nme2Smu nCas9 (D16A) E932R

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Nme2-Cas9, Smu PID, D16A E932R

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2700-CMV-Nme2-ABE8e-nt-E932R

Plasmid

#228721

Purpose

Expresses N-terminal ABE8e-Nme2 nCas9 (D16A) base editor with E932R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

N-terminal ABE8e with Nme2 nCas9 (D16A) E932R

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Nme2-Cas9 D16A E932R

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEXP5-NT/6xHis mCherry F30 Broccoli

Plasmid

#169234

Purpose

Constitutive T7 RNAP-mediated expression of 6xHis-mCherry with Broccoli RNA aptamer.

Depositor

Dora Tang

Article

Gonzales et al ACS Synth Biol. 2022 Jan 21;11(1):205-21

Insert

6xHis mCherry F30-Broccoli

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Dec. 23, 2021

Availability

Academic Institutions and Nonprofits only

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