Addgene

We narrowed to 1,687 results for: lin tian

Showing: 161 - 180 of 1687 results

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HT114_Fsyn_FAS(Cre off)_QuasAr6b_Citrine

Plasmid

#178825

Purpose

Neuronal expression (Cre-off) of an archaerhodopsin-derived genetically encoded voltage indicator QuasAr6b carrying a Citrine expression tag

Depositor

Adam Cohen

Article

Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

Insert

QuasAr6b_citrine

Use

Cre/Lox and Lentiviral

Tags

citrine

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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HT107_pAAV_hSyn-DiO-SomQuasAr6a_EGFP-P2A-somCheRiff_HA

Plasmid

#178826

Purpose

Cre-on bicistronic expression of QuasAr6b-based Optopatch under an neuronal promoter

Depositor

Adam Cohen

Article

Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

Insert

SomQuasAr6a_EGFP-P2A-somCheRiff_HA

Use

AAV, Cre/Lox, and Mouse Targeting

Tags

EGFP; HA

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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HT115_pAAV_hSyn-DiO-SomQuasAr6b_EGFP-P2A-somCheRiff_HA

Plasmid

#178827

Purpose

Cre-on bicistronic expression of QuasAr6b-based Optopatch under an neuronal promoter

Depositor

Adam Cohen

Article

Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

Insert

SomQuasAr6a_EGFP-P2A-somCheRiff_HA

Use

AAV, Cre/Lox, and Mouse Targeting

Tags

EGFP, HA

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pSin-EF2-LIN28-Pur

Plasmid

#16580

Depositor

James Thomson

Article

Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

Insert

Homo sapiens lin-28 homolog (C. elegans) (LIN28A Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 4, 2008

Availability

Academic Institutions and Nonprofits only

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CLYBL-TO-hNGN2-BSD-mApple

Plasmid

#124229

Purpose

Targets the human CLYBL safe harbor locus for integration of dox-inducible NGN2 for differentiation of iPSCs into cortical neurons. Contains a floxed BSD-NLS-mApple selection cassette.

Depositor

Michael Ward

Article

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

NGN2 (NEUROG2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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Human Whole Genome sgRNA iBAR Library

Pooled Library

#140633

Purpose

CRISPR pooled screen at high MOI by the embedded internal barcodes

Depositor

Wensheng Wei

Article

Zhu et al Genome Biol. 2019 Jan 24;20(1):20. doi:

Use

CRISPR and Lentiviral

Available since

April 21, 2020

Availability

Academic Institutions and Nonprofits only
pcDNA3_QLuc

Plasmid

#183260

Purpose

Express QLuc in mammalian cells

Depositor

Huiwang Ai

Article

Xiong et al J Am Chem Soc. 2022 Aug 1. doi: 10.1021/

Insert

QLuc

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pXG237

Plasmid

#141281

Purpose

ATF4 translational reporter

Depositor

Martin Kampmann

Article

Guo et al Nature. 2020 Mar;579(7799):427-432. doi:

Insert

uORF1/2 of ATF4 followed by mApple

Use

Lentiviral

Tags

mApple

Expression

Mutation

Promoter

CMV

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pXG260

Plasmid

#141282

Purpose

CMV:EGFP (as control for pXG237)

Depositor

Martin Kampmann

Article

Guo et al Nature. 2020 Mar;579(7799):427-432. doi:

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Sept. 19, 2020

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-zDrosha

Plasmid

#171540

Purpose

Encodes Zebrafish Drosha protein

Depositor

Yan Zeng

Article

Li et al Biochem Biophys Res Commun. 2019 Jun 18;

Insert

drosha (rnasen Zebrafish)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-BRIPO

Plasmid

#215542

Purpose

BRIPO (Bioluminescent Red Indicator for Potassium)

Depositor

Huiwang Ai

Article

Zhao et al J Am Chem Soc. 2024 May 15;146(19):13406

Insert

BRIPO

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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Syt1LeftArm-3XGS-GCaMP6s-SV40-3XP3-dsRed-SV40-Syt1RightArm

Plasmid

#159636

Purpose

Donor plasmid for generating Syt1:GCaMP6s strain in Aedes aegypti with CRISPR/Cas9

Depositor

Carolyn McBride

Article

Zhao et al Cell Rep Methods. 2021 Jul 26;1(3):10004

Insert

GCaMP6s

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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TL023_lenti_CMV:HaloTag-GluA1

Plasmid

#192518

Purpose

Expression of AMPA GluA1 with N terminal HaloTag fusion

Depositor

Adam Cohen

Article

Kim et al bioRxiv. 2023 May 29:2023.05.26.541296.

Insert

HaloTag-GluA1

Use

Lentiviral

Tags

HaloTag

Expression

Mutation

Promoter

CMV

Available since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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Brp-T2A-QF2w-Hsp70-3XP3-dsRed

Plasmid

#141094

Purpose

Template plasmid for inserting QF2w into the Brp gene of Aedes aegypti with CRISPR/Cas9, to generate a pan-neuronal driver line.

Depositor

Carolyn McBride

Article

Zhao et al Cell Rep Methods. 2021 Jul 26;1(3):10004

Insert

bruchpilot

Use

CRISPR

Tags

Expression

Insect

Mutation

coding sequence downstream of CRISPR cut site, sy…

Promoter

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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Ai38

Plasmid

#34883

Depositor

Hongkui Zeng

Article

Zariwala et al J Neurosci. 2012 Feb 29;32(9):3131-41. d

Insert

CAG-Floxed GCaMP3

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

CAG

Available since

March 7, 2012

Availability

Academic Institutions and Nonprofits only

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pET22b-T5-mad

Plasmid

#188998

Purpose

Express wildtype madanin-1

Depositor

Han Xiao

Article

Chen et al Nat Commun. 2022 Sep 16;13(1):5434. doi:

Insert

wildtype madanin-1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pBT120a17-sg-del

Plasmid

#214347

Purpose

Expresses pIII

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

gIII-xLuxAB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMBP-AsCas12a

Plasmid

#113430

Purpose

Expresses 10xHis-MBP fused to AsCas12a

Depositor

Jennifer Doudna

Article

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Insert

AsCas12a

Use

Tags

MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pMBP-FnCas12a

Plasmid

#113432

Purpose

Expresses 10xHis-MBP fused to FnCas12a

Depositor

Jennifer Doudna

Article

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Insert

FnCas12a

Use

Tags

MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pSK-Sasg

Plasmid

#214350

Purpose

Expresses cloning backbone for SlugCas9 sgRNA

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

SlugCas9 tracrRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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