Addgene

We narrowed to 2,001 results for: rp1

Showing: 161 - 180 of 2001 results

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pET28_6xHis-PARP1-WGR

Plasmid

#174808

Purpose

Bacterial expression of isolated PARP1 WGR domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-WGR (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Drp1 V2 S579D

Plasmid

#46343

Depositor

David Chan

Article

Loson et al Mol Biol Cell. 2013 Jan 2.

Insert

Drp1 V2 (Dnm1l Mouse)

Use

Tags

Expression

Mammalian

Mutation

S579D

Promoter

CMV

Available since

July 23, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-PARP1_D770A-mCherry

Plasmid

#192260

Purpose

Expresses PARP1 D770A mutant in mammalian cells. Tagged with mCherry.

Depositor

Michael Cohen

Article

Arnold et al Cell Chem Biol. 2022 Dec 15;29(12):1694-

Insert

PARP1_D770A (PARP1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

D770A

Promoter

CMV

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-PARP1_R878A-mCherry

Plasmid

#192261

Purpose

Expresses PARP1 R878A mutant in mammalian cells. Tagged with mCherry.

Depositor

Michael Cohen

Article

Arnold et al Cell Chem Biol. 2022 Dec 15;29(12):1694-

Insert

PARP1_R878A (PARP1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

R878A

Promoter

CMV

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Drp1 V2 S579A

Plasmid

#46342

Depositor

David Chan

Article

Loson et al Mol Biol Cell. 2013 Jan 2.

Insert

Drp1 V2 (Dnm1l Mouse)

Use

Tags

Expression

Mammalian

Mutation

S579A

Promoter

CMV

Available since

July 23, 2014

Availability

Academic Institutions and Nonprofits only
iRFP-PAmCherry1-PH_GRP1

Plasmid

#92168

Purpose

PI(3,4,5)P3 single molecule fluorescent reporter

Depositor

Xuelin Lou

Article

Ji et al J Biol Chem. 2015 Nov 6;290(45):26978-93

Insert

iRFP-PAmCherry1-PHgrp1

Use

Tags

iRFP-PAmCherry1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

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pFLAG-CMV2-CARP1

Plasmid

#16014

Depositor

Wafik El-Deiry

Article

McDonald et al Proc Natl Acad Sci U S A. 2004 Apr 20. 1

Insert

CARP1 (RNF34 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pET28_6xHis-PARP1-Y986H

Plasmid

#174801

Purpose

Bacterial expression of a PARP1 mutant that produces highly branched but short PAR chains overall

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-Y986H (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Y986H, V762A

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-Y986S

Plasmid

#174802

Purpose

Bacterial expression of a PARP1 mutant that produces mainly short PAR oligomers

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-Y986S (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Y986H, V762A

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-G972R

Plasmid

#174800

Purpose

Bacterial expression of a PARP1 mutant that produces less PAR oligomers overall

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-G972R (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

G972R, V762A

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET-DUET-Prp18

Plasmid

#111354

Purpose

protein purification

Depositor

Christine Guthrie

Insert

PRP18

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pBIS-GALkFLP(Trp1)

Plasmid

#49459

Purpose

overespression of Flp step-arrest mutant in yeast, TRP1 selection

Depositor

Marc Gartenberg

Article

Tsalik et al Yeast. 1998 Jun 30;14(9):847-52.

Insert

Flp(H305L) (flp Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

H305L, ‘step-arrest’ mutant,

Promoter

GAL10

Available since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C NLRP1 CARD WT

Plasmid

#164008

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 CARD

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 (NLRP1 Human)

Use

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 15, 2021

Availability

Academic Institutions and Nonprofits only

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Drp1(A395D)-StrepII

Plasmid

#174430

Purpose

Expresses human Drp1 Isoform 3 with A395D mutation in bacteria

Depositor

Thomas Pucadyil

Article

Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

Insert

Drp1

Use

Tags

StrepII

Expression

Bacterial

Mutation

A395D

Promoter

T7

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-HTB-TRP1

Plasmid

#26877

Depositor

Peter Kaiser

Article

Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

Insert

HTB tag

Use

Pcr tagging yeast

Tags

Expression

Mutation

Promoter

Available since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pFA6a-HBH-TRP1

Plasmid

#26874

Depositor

Peter Kaiser

Article

Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

Insert

HBH tag

Use

Pcr tagging yeast

Tags

Expression

Mutation

Promoter

Available since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pFA6a-PA-GFP-TRP1

Plasmid

#18821

Depositor

Wei-Lih Lee

Article

Vorvis et al Yeast. 2008 Sep;25(9):651-9.

Insert

Photoactivatable Green Fluorescent Protein

Use

Yeast tagging

Tags

Expression

Mutation

Promoter

Available since

July 27, 2008

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1delWGR-L713F

Plasmid

#173941

Purpose

Bacterial expression of PARP1 lacking WGR domain but containing L713F gain-of-function mutation

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delWGR-L713F (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 521-660; L713F, V762A

Promoter

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 NLRP1 A66V

Plasmid

#166817

Purpose

Gateway pDONR vector encoding NLRP1 with the patient mutation A66V (Zhong et al., 2016) (no stop codon)

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway shuttling vector

Tags

Expression

Mutation

A66V

Promoter

Available since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 NLRP1 S1213A

Plasmid

#166824

Purpose

Gateway pDONR vector containing NLRP1 with the P1214R patient mutation (no stop codon)

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway shuttling vector

Tags

Expression

Mutation

S1213A

Promoter

Available since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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