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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,137 results for: sequence

Showing: 161 - 180 of 13137 results

  1. pET28-NFMshuf2

    Plasmid
    #232059
    Purpose
    Expresses construct for surface tethering: R. norvegicus NFM tail domain with more evenly distributed charged residues, sequence 2
    Depositor
    Sanjay Kumar
    Article
    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121
    Insert
    Neurofilament-Medium tail domain, charge shuffle 2
    Use
    Tags
    6xHis, thrombin
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    March 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    232059-plasmid-map-sequence-id-464059

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  2. GB2890

    Plasmid
    #193133
    Purpose
    A2 Proximal promoter sequence consisting of the target sequence for the gRNA1 (GB1838) at "d site" (-120 from TSS) and gRNA2 (GB1839) flanked by random sequences.
    Depositor
    Diego Orzaez
    Article
    Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3
    Insert
    GB_SynP (A2) G1dG2b.1
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    193133-plasmid-map-sequence-id-380393

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  6. pGL004

    Plasmid
    #215336
    Purpose
    amyE integration vector for IPTG-inducible expression in Bacillus subtilis (Pspank-Hy promoter)
    Depositor
    Gene-Wei Li
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    hyper-pSpank
    Available Since
    March 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    215336-plasmid-map-sequence-id-426101

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  7. pAAV-CamKIIa-C1V1::FusionRed::Kv2.1

    Plasmid
    #102771
    Purpose
    "Activate neurons with 2p stimulation"
    Depositor
    Karel Svoboda
    Insert
    C1V1::FusionRed::Kv2.1
    Use
    AAV
    Tags
    FusionRed and Kv2.1 traficking domain
    Expression
    Mutation
    Promoter
    Available Since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    102771-plasmid-map-sequence-id-239474

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  8. pAG471 Lyn11-iFAST

    Plasmid
    #130817
    Purpose
    Expresses Lyn11-iFAST in mammalian cells (inner plasma membrane labeling)
    Depositor
    Arnaud Gautier
    Insert
    Lyn11-iFAST
    Use
    Tags
    myc-tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Sept. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    130817-plasmid-map-sequence-id-254955

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  9. pAP215.rAAV.mU6-sgRNA.Handle.EF1a-mTagBFP2

    Plasmid
    #217635
    Purpose
    AAV backbone for sgRNA expression. Contains a Lox-flanked handle sequence for Cre-dependent PCR amplification of sgRNAs. Protospacer is cloned between BstXI and BlpI.
    Depositor
    Martin Kampmann
    Article
    Ramani et al Nat Neurosci. 2025 Aug 22. doi: 10.1038/
    Insert
    mU6-sgRNA, Handle sequence, EF1a-mTagBFP2
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    EF1alpha and mU6
    Available Since
    Oct. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    217635-plasmid-map-sequence-id-449624

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  11. RANLS-LEHD-BNES

    Plasmid
    #60973
    Purpose
    Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-9 biosensor, used together with plasmid 2 (BNLS).
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    ddRFP A and ddRFP B
    Use
    Tags
    Caspase9 substrate sequence (LEHD) is after NLS s…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  15. pAcGFP1-mito

    Plasmid
    #174180
    Purpose
    Express AcGFP1 with mitochondria-targeting sequence in mammalian cells
    Depositor
    Yusuke Ohba
    Article
    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183
    Insert
    AcGFP with mitochondria-targeting sequence
    Use
    Tags
    the mitochondria-targeting sequence of the cytoch…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    174180-plasmid-map-sequence-id-354789

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  16. pFX-mito-EGFP

    Plasmid
    #159096
    Purpose
    Express EGFP with mitochondria-targeting sequence in mammalian cells
    Depositor
    Yusuke Ohba
    Article
    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183
    Insert
    mCherry with mitochondria-targeting sequence
    Use
    Tags
    the mitochondria-targeting sequence of the cytoch…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    159096-plasmid-map-sequence-id-354796

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  19. BtuF

    Plasmid
    #92209
    Purpose
    Expression of BtuF which is the vitamin B12 binding protein for BtuCD ABC Transporter
    Depositor
    Douglas Rees
    Article
    Borths et al Proc Natl Acad Sci U S A. 2002 Dec 24;99
    Insert
    BtuF (btuF )
    Use
    Tags
    6x His Tag and PelB Signal Sequence
    Expression
    Bacterial
    Mutation
    BtuF signal sequence removed and uses the PelB si…
    Promoter
    T7 Promoter
    Available Since
    July 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    92209-plasmid-map-sequence-id-183562

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Showing: 161 - 180 of 13137 results