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pDH22

Plasmid

#83798

Purpose

Useful for tagging yeast proteins with N-terminal YFP tag; G418 selection

Depositor

Eric Muller

Article

Hailey et al Methods Enzymol. 2002;351:34-49.

Insert

YFP

Tags

YFP

Expression

Yeast

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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C-Kras

Plasmid

#27807

Depositor

Steven Vogel

Article

Koushik et al J Biomed Opt. 2008 May-Jun;13(3):031204.

Insert

Cerulean Kras

Expression

Mammalian

Available Since

June 3, 2011

Availability

Academic Institutions and Nonprofits only
pFLIPmal-40uDelta1Venus

Plasmid

#18854

Depositor

Wolf Frommer

Article

Kaper et al Biotechnol Biofuels. 2008 Jun 3. 1(1):11

Insert

pFLIPmal-40uDelta1V

Tags

CFP and Venus

Expression

Bacterial

Mutation

N227A

Available Since

Oct. 23, 2008

Availability

Academic Institutions and Nonprofits only
pHRgfpLox

Plasmid

#12264

Depositor

Didier Trono

Article

Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

Insert

eGFP

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Mutation

EGFP, LoxP added to pHR' vector.

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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gBeta Cerulean

Plasmid

#27810

Depositor

Steven Vogel

Article

Thaler et al Biophys J. 2005 Oct . 89(4):2736-49.

Insert

mCerulean C1-Gbeta

Expression

Mammalian

Available Since

June 23, 2011

Availability

Academic Institutions and Nonprofits only
gGamma Venus

Plasmid

#27811

Depositor

Steven Vogel

Article

Thaler et al Biophys J. 2005 Oct . 89(4):2736-49.

Insert

mVenus C1-gGamma

Expression

Mammalian

Available Since

June 3, 2011

Availability

Academic Institutions and Nonprofits only
CTA

Plasmid

#27804

Depositor

Steven Vogel

Article

Koushik et al J Biomed Opt. 2008 May-Jun;13(3):031204.

Insert

Cerulean Traf Amber

Expression

Mammalian

Available Since

July 5, 2011

Availability

Academic Institutions and Nonprofits only
CTraf

Plasmid

#27808

Depositor

Steven Vogel

Article

Thaler et al Biophys J. 2005 Oct . 89(4):2736-49.

Insert

Cerulean Traf (TANK Human)

Expression

Mammalian

Available Since

June 23, 2011

Availability

Academic Institutions and Nonprofits only
VTA

Plasmid

#27805

Depositor

Steven Vogel

Article

Koushik et al J Biomed Opt. 2008 May-Jun;13(3):031204.

Insert

Venus Traf Amber

Expression

Mammalian

Available Since

July 5, 2011

Availability

Academic Institutions and Nonprofits only
pAG AAV CMV EGFP-HA

Plasmid

#242963

Purpose

Edit AAV plasmid expressing EGFP-HA, control for plasmid 242962

Depositor

Meghan Rego, Addgene Research Program

Insert

EGFP

Use

AAV

Tags

HA

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAG AAV CMV EGFP

Plasmid

#242959

Purpose

AAV plasmid expressing EGFP, control for plasmid 242957

Depositor

Meghan Rego, Addgene Research Program

Insert

EGFP

Use

AAV

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pGG-9.47_VP2_AS #2562

Plasmid

#74238

Purpose

Modified plasmid with a different backbone that yields higher titers of AAV-AS vectors. Encodes VP2-AS chimeric protein with 19 alanines fused to N-terminus of AAV9.47 VP2.

Depositor

Miguel Sena-Esteves

Article

Choudhury et al Mol Ther. 2015 Dec 28. doi: 10.1038/mt.2

Inserts

AAV2 Rep
AAV9.47 VP2-AS

Use

AAV

Available Since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

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pAR-9.47_VP2_AS

Plasmid

#74241

Purpose

Original plasmid described in Choudhury et al, 2015 Mol. Ther. for production of AAV-AS vectors. Encodes VP2_AS chimeric protein with 19 alanines fused to the N-terminus of AAV9.47 VP2.

Depositor

Miguel Sena-Esteves

Article

Choudhury et al Mol Ther. 2015 Dec 28. doi: 10.1038/mt.2

Inserts

AAV2 Rep
AAV9.47-VP2_AS

Use

AAV

Tags

19 alanines fused to VP2 of AAV9.47

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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pAG AAV CMV HA-EGFP

Plasmid

#242961

Purpose

AAV plasmid expressing HA-EGFP, control for plasmid 242960

Depositor

Meghan Rego, Addgene Research Program

Insert

EGFP

Use

AAV

Tags

HA

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pRL443

Plasmid

#70261

Purpose

Shuttle vector suitable for mobilizing plasmids to Anabaena (Nostoc) sp. strain PCC 7120 and other cyanobacterial strains.

Depositor

Peter Wolk

Article

Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.

Type

Empty backbone

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pBHt2

Plasmid

#104175

Purpose

Empty backbone for Agrobacterium tumefaciens-mediated transformation of Fusarium oxysporum

Depositor

Seogchan Kang

Article

Mullins et al Phytopathology. 2001 Feb;91(2):173-80. d

Type

Empty backbone

Use

Fungal expression

Promoter

none

Available Since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pLOX-gfp-iresTK

Plasmid

#12243

Depositor

Didier Trono

Article

Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

Insert

GFP, HSV1-TK

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Mutation

LoxP in 3' LTR.

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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BBa_J72214-BBa_J72090

Plasmid

#40782

Depositor

Christopher Anderson

Article

Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

Insert

VioABCDE

Use

Synthetic Biology; Phagemid

Expression

Bacterial

Available Since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only

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pET28CLY1

Plasmid

#21760

Depositor

Maarten Merkx

Article

Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

Insert

ECFP -EYFP linked by flexible one SGGSGG repeats

Tags

His

Expression

Bacterial

Available Since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only

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Duet011

Plasmid

#17627

Depositor

Linzhao Cheng

Article

Zhou et al Stem Cells. 2007 Mar . 25(3):779-89.

Insert

UBC promoter - GFP - PGK promoter - Hygro

Use

Lentiviral

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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