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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,270 results for: Streptococcus

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Showing: 1781 - 1800 of 3270 results

  1. dead Sa Cas9

    Plasmid
    #138162
    Purpose
    For orthogonal R loop assay (use with Sp-derived base editor, Sp gRNA, and Sa gRNA for assay)
    Depositor
    David Liu
    Publication
    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dSaCas9
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    D10A + N580A
    Promoter
    CMV
    Available since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    138162-plasmid-map-sequence-id-272344

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  2. xCas9(3.6)-BE3

    Plasmid
    #108385
    Purpose
    Mammalian expression vector for xCas9(3.6)-BE3
    Depositor
    David Liu
    Publication
    Hu et al Nature. 2018 Feb 28. pii: nature26155. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    xCas9(3.6)-BE3
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    E108G, S217A, A262T, S409I, E480K, E543D, M694I, …
    Available since
    March 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    108385-plasmid-map-sequence-id-207181

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  4. pKMD111e - intein-Cas9(S219-G521R)

    Plasmid
    #64192
    Purpose
    Expresses intein-Cas9(S219-G521R) in mammalian cells
    Depositor
    David Liu
    Publication
    Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized S. pyogenes intein-Cas9(S219-G521R) - NLS - 3x FLAG
    Use
    CRISPR
    Tags
    NLS - 3x FLAG (C terminal on insert)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    64192-plasmid-map-sequence-id-107551

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  5. pCX419

    Plasmid
    #229216
    Purpose
    All-in-one plasmid for labeling of XIST RNA via Csm complex
    Depositor
    Jennifer Doudna
    Publication
    Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Csm1; Csm2, Csm3(RNase mut)-sfGFP; Csm4; Csm5; Cas6; XIST crRNA
    Expression
    Mammalian
    Available since
    Feb. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    229216-plasmid-map-sequence-id-457179

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  7. pCMV-eNme2-C.NR

    Plasmid
    #185672
    Purpose
    Expresses nuclease active eNme2-C.NR in mammalian cells
    Depositor
    David Liu
    Publication
    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eNme2-C.NR nuclease
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    eNme2-C S6P/G33E/A520E/S646R/V696F/R711G/V758I/Y7…
    Available since
    Sept. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    185672-plasmid-map-sequence-id-364078

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  11. M-SP-sgRNA

    Plasmid
    #48671
    Purpose
    Mammalian U6-driven sgRNA (SPm) targeting GTCCCCTCCACCCCACAGTG
    Depositor
    George Church
    Publication
    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with S. pyogenes Cas9, hU6 promoter
    Use
    CRISPR
    Promoter
    hU6
    Available since
    Nov. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    48671-plasmid-map-sequence-id-71841

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  15. SECURE BE3(R33A)-P2A-EGFP (pJUL1085)

    Plasmid
    #123614
    Purpose
    CAG promoter expression plasmid for rAPOBEC1(R33A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A).
    Depositor
    Keith Joung
    Publication
    Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BE3(R33A)-P2A-EGFP
    Expression
    Mammalian
    Mutation
    R33A in rAPOBEC1, D10A in SpCas9
    Promoter
    CAG
    Available since
    April 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    123614-plasmid-map-sequence-id-239342

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  18. pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)

    Plasmid
    #140082
    Purpose
    Entry cloning vector for in vitro transcription or expression of SpCas9 sgRNAs from a T7 promoter
    Depositor
    Keith Joung
    Insert
    pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
    Use
    In vitro transcription (mrna synthesis)
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    140082-plasmid-map-sequence-id-292704

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  19. CROPseq-EFS-HypaSpCas9-P2A-puro

    Plasmid
    #106965
    Purpose
    single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen
    Depositor
    Alex Hewitt
    Insert
    HypaSpCas9
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    April 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    106965-plasmid-map-sequence-id-207982

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  20. pdCas9-DNMT3A-PuroR_hNT

    Plasmid
    #71830
    Purpose
    Expression of dCas9-DNMT3A fusion with T2A-PuroR and non-targeting control sgRNA for use in human cells
    Depositor
    Vlatka Zoldos
    Publication
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Non-targeting sgRNA human (DNMT3A Synthetic, S. pyogenes, Human)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    U6
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    71830-plasmid-map-sequence-id-131025

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Showing: 1781 - 1800 of 3270 results