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pYEXC3GH

Plasmid

#49027

Purpose

FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tag

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Yeast

Promoter

GAL1

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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JBEI-12050

Plasmid

#84982

Purpose

Isopentenol production

Depositor

Taek Soon Lee

Article

Kang et al Metab Eng. 2016 Mar;34:25-35. doi: 10.10

Insert

MevTo-T21-MK-aphA

Expression

Bacterial

Promoter

LacUV5

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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promoter-less mRFP1E

Plasmid

#183144

Purpose

BioBrick pSB1C3 plasmid encoding a promoter-less version of mRFP1E

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

RBS, mRFP1E

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available Since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-C-Myc-DDK-IRES-Puro HNRNPR

Plasmid

#200730

Purpose

Lentiviral expression of Flag-Tagged human HNRNPR

Depositor

Claudio Alarcón

Insert

HNRNPR (HNRNPR Human)

Use

Lentiviral

Tags

Myc-DDK from backbone plasmid

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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LIIbeta F 3-4

Plasmid

#54360

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Type

Empty backbone

Use

Plant expression

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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LIIbeta F 4-5

Plasmid

#54362

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Type

Empty backbone

Use

Plant expression

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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LIIIalpha fin

Plasmid

#54381

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Type

Empty backbone

Use

Plant expression

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pT7_circRAJ31

Plasmid

#69931

Purpose

Expresses RAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. Under control of a T7 promoter.

Depositor

Alfonso Jaramillo

Article

Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do

Insert

pT7_circRAJ31

Use

Synthetic Biology

Promoter

T7

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pCG-B-SpyTag-C

Plasmid

#87373

Purpose

An ORF part-containing vector harbouring the SpyTag sequence with 4 bp overhangs specific for the ‘upstream SpyPart’ position within an ORF assembly

Depositor

Tom Ellis

Article

Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

Insert

SpyTag

Use

Synthetic Biology

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pGL2_136

Plasmid

#199083

Purpose

Even ORI-marker screen destination vector with sfYFP dropout for barcoded-ORI insertion

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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