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pRSET-TEV-12His

Plasmid

#137032

Purpose

empty vector; C-terminal TEV-protease-cleavable His12; T7 promoter; BseRI cloning sites (e.g., In-Fusion compatible)

Depositor

Debra Hansen

Article

Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

Type

Empty backbone

Tags

His12 (TEV protease cleavable)

Expression

Bacterial

Promoter

T7

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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J23103_GB

Plasmid

#65991

Purpose

MoClo Basic Part: Constitutive promoter - Anderson series - low strength [G:J23103:B]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Constitutive promoter

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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J23107_GB

Plasmid

#65999

Purpose

MoClo Basic Part: Constitutive promoter - Anderson series - medium strength [G:J23107:B]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Constitutive promoter

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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J23116_GB

Plasmid

#66003

Purpose

MoClo Basic Part: Constitutive promoter - Anderson series - low strength [G:J23116:B]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Constitutive promoter

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pEGB Tnos:NptII:Pnos (GB0226)

Plasmid

#68212

Purpose

Transcriptional unit for kanamycin resistance gene plant expression

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

NptII

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

Pnos

Available Since

Oct. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pHsp70B (GB0155)

Plasmid

#68172

Purpose

Provides the Arabidopsis thaliana heat shock protein 70B promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtHsp70B promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pKH163_pSmart-mNEON-YIPF4

Plasmid

#214982

Purpose

endogenous mneon tag for YIPF4

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

mNEON with YIPF4 homology (YIPF4 Human)

Use

CRISPR

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-TUBB4B

Plasmid

#207785

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of TUBB4B for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of TUBB4B (TUBB4B Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pRDA_791

Plasmid

#216079

Purpose

Minimal plasmid; contains only ori and ampR, destination vector

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Destination

Expression

Bacterial

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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AA295

Plasmid

#215986

Purpose

Fragmid fragment: (N' terminus) SV40 Nuclear Localization Signal

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; SV40NLS_v1.19

Use

Fragment

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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