We narrowed to 2,168 results for: PAM

1. pAGH51

   Plasmid

   #107255

   Purpose

   HU-FRB construct (anchor) expressed in trans from the slr0168 neutral site of Synechocystis PCC6803. Used to anchor away any FKBP tagged target in presence of rapamycin.

   Depositor

   Erin O'Shea

   Article

   Gutu et al Mol Microbiol. 2018 Jan 22. doi: 10.1111

   Insert

   HU-FRB
   

   Use

   Synthetic Biology

   Tags

   FRB

   Promoter

   native HU promoter

   Available Since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. NmCAT

   Plasmid

   #241909

   Purpose

   Heterologous expression of 4-hydroxybutyrate-CoA synthetase from Nitrosopumilis maritimus

   Depositor

   Neil Dixon

   Article

   Zheng et al Biochem. Eng. J. 2024 Sep 16; 212. doi:

   Insert

   4HB-CoA synthetase
   

   Expression

   Bacterial

   Mutation

   Mutated Haloferax gyrase B (gyrB*)

   Available Since

   Aug. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pXD80ENSF30-DadhABC(cHis8)RS-Accs(Cadh)(ElA2)

   Plasmid

   #233876

   Purpose

   Expresses Eggerthella lenta A2 Dadh with the accessory genes from the cadh gene cluster in Gordonibacter urolithinfaciens DSM 27213

   Depositor

   Emily Balskus

   Article

   Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

   Insert

   Dadh
   

   Tags

   His8

   Expression

   Bacterial

   Available Since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. FKBP-EGFP-PML peptide

   Plasmid

   #175247

   Purpose

   Bacterial expression and purification, low affinity SUMOylation substrate with a relatively high Km, can be recruited to FRB with rapamycin

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   PML (PML Human)
   

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. PD30

   Plasmid

   #164543

   Purpose

   Expresses Drd1-Ub

   Depositor

   Gregory Pazour

   Article

   Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

   Insert

   Dopamine receptor D1 (Drd1 Mouse)
   

   Use

   Lentiviral

   Tags

   FLAG-Ub

   Mutation

   WT

   Promoter

   CMV

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Rap-MESA-20-Tev

   Plasmid

   #84555

   Purpose

   MESA protease chain with rapamycin FKBP ectodomain, 20 extracellular linkers, and Tev protease

   Depositor

   Joshua Leonard

   Article

   Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

   Insert

   Rap-MESA-FKBP-20-Tev
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV2-GCaMP5g-P2A-Vmn1r89

   Plasmid

   #133416

   Purpose

   Fluorescent calcium reporter and mouse vomeronasal 1 receptor 89

   Depositor

   Tim Holy

   Article

   Lee et al Science. 2019 Dec 13;366(6471):1384-1389

   Insert

   GCaMP5g-P2A-PAmCherry-Vmn1r89 (Vmn1r89 Mouse)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. P0050::kaiBC S431A T432A NS1

   Plasmid

   #101837

   Purpose

   drives expression of kaiBC S431A T432A variant from an rpaA-independent promoter in NS1 locus

   Depositor

   Erin O'Shea

   Article

   Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

   Insert

   kaiBC S431A T432A with p0050 promoter
   

   Use

   Cyanobacteria cloning vector

   Mutation

   S431A T432A

   Available Since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. P0050::kaiBC S431E T432E NS1

   Plasmid

   #101836

   Purpose

   drives expression of kaiBC S431E T432E variant from an rpaA-independent promoter in NS1 locus

   Depositor

   Erin O'Shea

   Article

   Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

   Insert

   kaiBC S431E T432E with p0050 promoter
   

   Use

   Cyanobacteria cloning vector

   Mutation

   S431E T432E

   Available Since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. p3E-FRB(KTF)-HA no-pA

    Plasmid

    #82596

    Purpose

    3' entry vector containing HA epitope tag fused to C-terminus of FRB mutant (W2101F) without pA for rapamycin/rapalog induced dimerization

    Depositor

    Kryn Stankunas

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    FRB-HA (no pA)
    

    Mutation

    W2101F

    Promoter

    None

    Available Since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPS835

    Plasmid

    #8933

    Depositor

    Pamela Silver

    Article

    Schlenstedt et al Proc Natl Acad Sci U S A 1995 Jan 3;92(1

    Insert

    GSP1 (GSP1 Budding Yeast)
    

    Tags

    myc

    Expression

    Yeast

    Mutation

    G21V, stabilizes the GTP-bound form

    Available Since

    Aug. 19, 2005

    Availability

    Academic Institutions and Nonprofits only

12. pJK412

    Plasmid

    #155371

    Purpose

    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJK430

    Plasmid

    #155377

    Purpose

    PphlF_A2NT in dCRv1, 2x cascade + [dCas9 + PA4-mVenus]

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    PphlF_A2NT in dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT3TS-spRYCas9-ABE-UMax

    Plasmid

    #222139

    Purpose

    Near PAMless Adenine base editor with modified TadA variant for A-to-G base editing in zebrafish

    Depositor

    Gaurav Varshney

    Article

    Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

    Insert

    TadA-nSpRYCas9
    

    Use

    CRISPR; Zebrafish expression

    Tags

    bipartite NLS

    Promoter

    T3

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. FKBP-CD28TMD-NTEVp(H75S)

    Plasmid

    #137828

    Purpose

    MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75S mutant TEV protease

    Depositor

    Joshua Leonard

    Article

    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

    Insert

    FKBP-CD28TMD-NTEVp(H75S)
    

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Mutated Histidine 75 to Serine in NTEVp fragment

    Promoter

    CMV

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. FRB-NLuc

    Plasmid

    #31181

    Depositor

    David Piwnica-Worms

    Article

    Luker et al Proc Natl Acad Sci U S A. 2004 Aug 17. 1

    Insert

    Rapamycin-binding domain and the N-terminus of Firefly Luciferase (MTOR Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET32a-TRXtag

    Plasmid

    #11516

    Depositor

    Pamela Bjorkman

    Article

    Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

    Insert

    None - thioredoxin is vector encoded (trxA )
    

    Tags

    His, His (out of frame), and Trx

    Expression

    Bacterial

    Mutation

    vector modified; removed N-terminal His tag by di…

    Available Since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

18. FRB_nSuMMVp

    Plasmid

    #118970

    Purpose

    Expresses N-terminus of sunflower mild mosaic virus protease (SuMMVp) fused to the FKBP-rapamycin binding (FRB) domain

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split nSuMMVp in fusion with FRB
    

    Tags

    Myc tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCPP5052

    Plasmid

    #128729

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

    Insert

    hopAM1
    

    Expression

    Bacterial

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. FRB-CD28TMD-CTEVp(WT)-CS(M)-tTA

    Plasmid

    #137827

    Purpose

    MESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal WT TEV protease, M cleavage sequence, and tTA transcription factor

    Depositor

    Joshua Leonard

    Article

    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

    Insert

    FRB-CD28TMD-CTEVp(WT)-CS(M)-tTA
    

    Tags

    3x FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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