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pBRPyCAG-Mettl3-dsRed-IRES-puro

Plasmid

#61516

Purpose

Constitutive expression of murine wild-type Mettl3

Depositor

Jacob Hanna

Article

Geula et al Science. 2015 Feb 27;347(6225):1002-6. d

Insert

Mettl3 (Mettl3 Mouse)

Expression

Mammalian

Promoter

CAGG

Available Since

Jan. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1) IGF1R

Plasmid

#107499

Purpose

Expresses IGF1R, puromycin resistant

Depositor

Alejandro Gutierrez

Article

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

IGF-1R (IGF1R Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1) FAM149A

Plasmid

#107510

Purpose

Expressing FAM149a, puromycin resistant

Depositor

Alejandro Gutierrez

Article

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

FAM149A (FAM149A Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1) CRIP2

Plasmid

#107509

Purpose

Expressing CRIP2, puromycin resistant

Depositor

Alejandro Gutierrez

Article

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

CRIP2 (codon optimized) (CRIP2 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Codon optimized sequence

Promoter

CMV

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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C321.ΔA.M9adapted

Bacterial Strain

#98568

Purpose

Recoded strain C321.ΔA adaptively evolved for 1000+ generations in M9 minimal media + 1% glucose and biotin

Depositor

George Church

Article

Wannier et al Proc Natl Acad Sci U S A. 2018 Mar 20;11

Bacterial Resistance

Ampicillin

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only
delta guaB

Bacterial Strain

#113653

Purpose

Base strain that the pDCAF plasmids (Addgene #113646-113652) must be in.

Depositor

Jeffrey Barrick

Article

Gutierrez et al Appl Environ Microbiol. 2019 Nov 14;85(2

Bacterial Resistance

Kanamycin

Available Since

Nov. 18, 2019

Availability

Industry, Academic Institutions, and Nonprofits
S1030

Bacterial Strain

#105063

Purpose

host strain for phage assisted continuous evolution

Depositor

David Liu

Article

Carlson et al Nat Chem Biol. 2014 Mar;10(3):216-22. do

Bacterial Resistance

Streptomycin and Tetracycline

Available Since

March 21, 2018

Availability

Academic Institutions and Nonprofits only
S2060

Bacterial Strain

#105064

Purpose

host strain for phage assisted continuous evolution

Depositor

David Liu

Article

Hubbard et al Nat Methods. 2015 Oct;12(10):939-42. doi

Bacterial Resistance

Streptomycin and Tetracycline

Available Since

March 23, 2018

Availability

Academic Institutions and Nonprofits only
pWPXLd

Plasmid

#12258

Depositor

Didier Trono

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pCDF-mcherry1

Plasmid

#87144

Purpose

Constitutive E. coli mcherry Expression

Depositor

Michael Lynch

Insert

mcherry

Expression

Bacterial

Promoter

Constitutive proD

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pWPXLd

Plasmid

#12258

Depositor

Didier Trono

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pWPT-GFP

Plasmid

#12255

Depositor

Didier Trono

Insert

EGFP

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pWPT-nlsLacZ

Plasmid

#12261

Depositor

Didier Trono

Insert

LacZ

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pWPXL

Plasmid

#12257

Purpose

2nd generation lentiviral transfer plasmid

Depositor

Didier Trono

Type

Empty backbone

Use

Cre/Lox and Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pCDF-mcherry1

Plasmid

#87144

Purpose

Constitutive E. coli mcherry Expression

Depositor

Michael Lynch

Insert

mcherry

Expression

Bacterial

Promoter

Constitutive proD

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCDF-mcherry2

Plasmid

#87145

Purpose

Constitutive E. coli mcherry Expression, with DAS4 tag

Depositor

Michael Lynch

Insert

mcherry

Tags

C terminal DAS4 tag

Expression

Bacterial

Promoter

Constitutive proD

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pWPT-GFP

Plasmid

#12255

Depositor

Didier Trono

Insert

EGFP

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pWPT-nlsLacZ

Plasmid

#12261

Depositor

Didier Trono

Insert

LacZ

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pWPXL

Plasmid

#12257

Purpose

2nd generation lentiviral transfer plasmid

Depositor

Didier Trono

Type

Empty backbone

Use

Cre/Lox and Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pJBL7071

Plasmid

#167240

Purpose

sfGFP reporter output for cell-free expression

Depositor

Julius Lucks

Insert

pT7-sfGFP

Use

Synthetic Biology

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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