We narrowed to 152,678 results for: Des

1. pCAG-T7-TALEN(Sangamo)-Destination

   Plasmid

   #37184

   Purpose

   Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses homodimeric FokI domains.

   Depositor

   Pawel Pelczar

   Article

   Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791

   Type

   Empty backbone

   Use

   Tags

   FokI nuclease

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 24, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST-DHFR F[1,2]-N (TRP1)

   Plasmid

   #177797

   Purpose

   Gateway destination vector to insert genes of interest having a N-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.

   Depositor

   Christian Landry

   Article

   Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

   Type

   Empty backbone

   Use

   Tags

   DHFR F[1,2]

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. 370 pTol2-lmo2:CherryDest

   Plasmid

   #73489

   Purpose

   Tol2 vector with lmo2 promoter upstream of Cherry, attR1/R2 cassette, and late SV40 pA signal

   Depositor

   Nathan Lawson

   Article

   Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

   Insert

   lmo2 promoter:mCherry-attR1-attR2
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST-FRT/TO-3xFLAG-REV7

   Plasmid

   #114127

   Purpose

   Expresses N-terminally tagged 3x FLAG-REV7 in mammalian cells

   Depositor

   Daniel Durocher

   Article

   Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

   Insert

   REV7 (MAD2L2 Human)
   

   Use

   Genomic integration, tetracycline inducible

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TetO2

   Available since

   Aug. 16, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pMuLE Lenti Dest eGFP

   Plasmid

   #62175

   Purpose

   MuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses EGFP under the control of a PGK promoter. Compatible with MultiSite Gateway cloning

   Depositor

   Ian Frew

   Article

   Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

   Type

   Empty backbone

   Use

   Lentiviral; Mule gateway destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST40-2XFL-Src-S4-S32-KD

   Plasmid

   #140299

   Purpose

   Expresses Flag-tagged human Src-S4-S32-KD in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src deletion mutant (SRC Human)
   

   Use

   Tags

   2X Flag

   Expression

   Mammalian

   Mutation

   lacks the UD region

   Promoter

   CMV

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST40-2XFL-Src-S32-KD

   Plasmid

   #140311

   Purpose

   Expresses Flag-tagged human Src-S32-KD in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src deletion mutant (SRC Human)
   

   Use

   Tags

   2X Flag

   Expression

   Mammalian

   Mutation

   Contains only the SH3, SH2, and KD regions

   Promoter

   CMV

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFRT/TO/FLAG/HA-DEST TNRC6C

   Plasmid

   #19885

   Depositor

   Thomas Tuschl

   Article

   Landthaler et al RNA. 2008 Oct 31. ():.

   Insert

   Trinucleotide repeat containing 6C (TNRC6C Human)
   

   Use

   Tags

   FLAG/HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST40-2XHA-Src-K298M

   Plasmid

   #140314

   Purpose

   Expresses HA-tagged human Src-K298M in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src mutant (SRC Human)
   

   Use

   Tags

   2X HA

   Expression

   Mammalian

   Mutation

   K298M

   Promoter

   CMV

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. 362 pCS Cherry DEST

    Plasmid

    #13075

    Depositor

    Nathan Lawson

    Article

    Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

    Insert

    Cherry
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCK033 p5E desma MCS

    Plasmid

    #195948

    Purpose

    Gateway p5E vector

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    desmin a regulatory elements (desma Zebrafish)
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMuLE Lenti Dest iRFP

    Plasmid

    #62176

    Purpose

    MuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses iRFP under the control of a PGK promoter. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    Lentiviral; Mule gateway destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST14-SpyCatcher003-TEVs-SpyTag003DA

    Plasmid

    #216283

    Purpose

    Expresses SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow TEV protease to remove SpyTag003DA and thereby uncage SpyCatcher003 reactivity.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    SpyCatcher003-TEVs-SpyTag003DA
    

    Use

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDEST-GST-FBW7 alpha

    Plasmid

    #197456

    Purpose

    The plasmid expresses GST-tagged FBW7 human isoform 1. Used for in vitro translation of FBW7alpha.

    Depositor

    Omar Khan

    Article

    Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:

    Insert

    FBW7 (FBXW7 Human)
    

    Use

    In vitro translation

    Tags

    GST

    Expression

    Bacterial

    Mutation

    See depositor comments below

    Promoter

    T7

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST-mCherry-LacR-BRCA1

    Plasmid

    #71115

    Purpose

    mammalian expression of mCherry-LacR-BRCA1 siRNA resistant construct

    Depositor

    Daniel Durocher

    Article

    Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TO

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. 223 pCS EGFP DEST

    Plasmid

    #13071

    Depositor

    Nathan Lawson

    Article

    Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFRT/TO/FLAG/HA-DEST PABPC4

    Plasmid

    #19882

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Oct 31. ():.

    Insert

    Poly(A) binding protein, cytoplasmic 4 (PABPC4 Human)
    

    Use

    Tags

    FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. LB901: pMVP/PB/Blast-DEST

    Plasmid

    #121873

    Purpose

    pMVP destination vector, empty PiggyBac transposon vector backbone w/ Blast selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Piggybac transposon, pmvp gateway destination vec…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMuLE Lenti Dest Neo

    Plasmid

    #62178

    Purpose

    MuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses Neomycin cassette under the control of a PGK promoter. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    Lentiviral; Mule gateway destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDEST40-2XFL-Src-K298M

    Plasmid

    #140315

    Purpose

    Expresses Flag-tagged human Src-K298M in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src mutant (SRC Human)
    

    Use

    Tags

    2X Flag

    Expression

    Mammalian

    Mutation

    K298M

    Promoter

    CMV

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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