We narrowed to 23,544 results for: CHI

1. pT2Aneo-ERT2CreERT2

   Plasmid

   #173850

   Purpose

   Encoding Cre recombinase (activated in response to tamoxifen)

   Depositor

   Michiyuki Matsuda

   Article

   Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

   Insert

   ERT2CreERT2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. myc-BioID-Rab7

   Plasmid

   #121049

   Purpose

   Expresses myc tagged BioID and human Rab7a in mammalian cells

   Depositor

   Gia Voeltz

   Article

   Hoyer et al Cell. 2018 Sep 20;175(1):254-265.e14. do

   Inserts

   BioID
   Rab7A
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   R118G (highly promiscuous form)

   Promoter

   CMV

   Available since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. NLS pEGFP-N3 (1299)

   Plasmid

   #62043

   Purpose

   mammalian expression of NLS-EGFP

   Depositor

   Eric Schirmer

   Article

   Zuleger et al Genome Biol. 2013 Feb 15;14(2):R14. doi:

   Insert

   SV40 NLS
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pBS-KLF7-200-GFPfr1

   Plasmid

   #169799

   Purpose

   THe donor vector for the KLF7 gene locus.

   Depositor

   Natsuko Chiba

   Article

   Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

   Insert

   Donor sequence to KLF7 neighbouring region for HR
   

   Use

   Hr donor vector for human actb gene.

   Tags

   Expression

   Mutation

   Partial sequence of GFP is inserted the sequence …

   Promoter

   Available since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. LentiCRISPRv2-KLF7-1

   Plasmid

   #169797

   Purpose

   Expresses Cas9 and guide RNA for the site neighbouring KLF7 gene.

   Depositor

   Natsuko Chiba

   Article

   Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

   Insert

   guide RNA for KLF7 neighbouring region
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLV-SI-121

   Plasmid

   #131126

   Purpose

   A-to-G base editing reporter

   Depositor

   Nozomu Yachie

   Article

   Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   mutant EGFP
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Sept. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGBW-m4046757

   Plasmid

   #145657

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. THI VP64 pALD4 T-

   Plasmid

   #161481

   Purpose

   Non targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pALD4_THi11_T-_gRNA_MS2

   Depositor

   Brigitte Gasser, Diethard Mattanovich

   Article

   Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

   Inserts

   dCAS9
   MS2-VP64
   gRNA (Non targeting control NTC)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pALD4, pPOR1, and pTEF2

   Available since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. RINS1(mut)

   Plasmid

   #107291

   Purpose

   Plasmid for the expression of the inactive mutant of RINS1 sensor

   Depositor

   Dmytro Yushchenko

   Article

   Schifferer et al Cell Chem Biol. 2017 Apr 20;24(4):525-53

   Insert

   RINS1(mut)
   

   Use

   Tags

   sfGFP

   Expression

   Mammalian

   Mutation

   two Arg/Ser mutations at the beginning and in the…

   Promoter

   CMV

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMM2-hPMCA2w/b

    Plasmid

    #47583

    Depositor

    Emanuel Strehler

    Article

    Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

    Insert

    PMCA2w/b (ATP2B2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMM2-hPMCA2z/b

    Plasmid

    #47581

    Depositor

    Emanuel Strehler

    Article

    Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

    Insert

    PMCA2z/b (ATP2B2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMRX-INU-TEX264 LIR4A-FLAG

    Plasmid

    #128259

    Purpose

    Expresses TEX264 LIR4A in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

    Insert

    TEX264 LIR4A (TEX264 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed FEEL (aa 273-276) to alanines

    Promoter

    CMV

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Tmem214 pmRFP-N2 (1348)

    Plasmid

    #62047

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

    Insert

    Tmem214 (TMEM214 Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3.2/V5 hsa-mir-192

    Plasmid

    #26311

    Depositor

    David Bartel

    Article

    Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

    Insert

    hsa-mir-192 (MIR192 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pMpGE_En01

    Plasmid

    #71534

    Purpose

    Used to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorpha

    Depositor

    Ikuko Hara-Nishimura, Takayuki Kohchi

    Article

    Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDONR221 CARD8

    Plasmid

    #169962

    Purpose

    pDONR221 for Gateway shuttling. Contains the CARD8 T60 isoform (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Gateway cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPB-CAG-Tfe3::ERT2-pgk-hph

    Plasmid

    #48756

    Purpose

    PiggyBac backbone, CAG promoter driving murine Tfe3-ERT2 fusion, Hygromycin selection

    Depositor

    Austin Smith

    Article

    Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

    Insert

    Tfe3::ERT2 (Tcfe3 Mouse)
    

    Use

    Piggybac

    Tags

    ERT2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

18. PKG I alpha dNPS

    Plasmid

    #16391

    Depositor

    Bernard Weinstein

    Article

    Deguchi et al Mol Cancer Ther. 2002 Aug . 1(10):803-9.

    Insert

    PKG1 alpha (PRKG1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    N-terminal pseudosubstrate domain deletion: delet…

    Promoter

    Available since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

19. Tmem214 pEGFP-N2 (1072)

    Plasmid

    #62026

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

    Insert

    Tmem214 (TMEM214 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

20. Luc-SIRT1 mutant 3'UTR

    Plasmid

    #20380

    Depositor

    Charles Lowenstein

    Article

    Yamakuchi et al Proc Natl Acad Sci U S A. 2008 Sep 9. 10

    Insert

    SIRT1 (SIRT1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    mutant 3'UTR of SIRT1 (ACACCCACCAAGGACCATTAG…

    Promoter

    Available since

    May 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. px330-UFM1 sgRNA2

    Plasmid

    #134634

    Purpose

    contains sgRNA targeting human UFM1 for gene knockout

    Depositor

    Yihong Ye

    Article

    Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

    Insert

    UFM1 sgRNA2 (UFM1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. px330-UFM1 sgRNA1

    Plasmid

    #134633

    Purpose

    contains sgRNA targeting human UFM1 for gene knockout

    Depositor

    Yihong Ye

    Article

    Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

    Insert

    UFM1 sgRNA1 (UFM1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCMV-YdLight1

    Plasmid

    #125705

    Purpose

    Expresses the yellow genetically encoded dopamine sensor YdLight1 in mammalian cells.

    Depositor

    Lin Tian

    Article

    Patriarchi et al Nat Methods. 2020 Nov;17(11):1147-1155.

    Insert

    YdLight1
    

    Use

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLentiCRISPRv2-dADAM17 (#1)

    Plasmid

    #177259

    Purpose

    A knockout vector for the dog Adam17

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    A gRNA targeting the dog Adam17 gene and the cDNA of CRISPR-Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEn-C1.1

    Plasmid

    #61479

    Purpose

    Encodes CRISPR sgRNA for Gateway or conventional cloning of 1-3 sgRNAs into pDe-CAS9 or pCAS9-TPC

    Depositor

    Holger Puchta

    Article

    Schiml et al Plant J. 2014 Oct 18. doi: 10.1111/tpj.1

    Insert

    AtU6-26:sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    U6-26

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.2/V5 hsa-mir-124

    Plasmid

    #26306

    Depositor

    David Bartel

    Article

    Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

    Insert

    hsa-mir-124 (MIR124-1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

27. pGEX-E6AP Hect

    Plasmid

    #165102

    Purpose

    bacterial expression of a GST fusion of Human E6AP Hect domain

    Depositor

    Arthur Haas

    Article

    Ronchi et al Methods Mol Biol. 2012;832:197-218. doi:

    Insert

    E6AP Hect (UBE3A Human)
    

    Use

    Tags

    GST from plasmid.

    Expression

    Bacterial

    Mutation

    E6AP Hect domain Amino acids 495-738 (5'QLNP…

    Promoter

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.2/V5 hsa-mir-205

    Plasmid

    #26312

    Depositor

    David Bartel

    Article

    Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

    Insert

    hsa-mir-205 (MIR205 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

29. pcDNA3.2/V5 hsa-mir-9-1

    Plasmid

    #26317

    Depositor

    David Bartel

    Article

    Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

    Insert

    hsa-mir-9-1 (MIR9-1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLV-SI-112

    Plasmid

    #131127

    Purpose

    C-to-T base editing reporter

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    Mutant EGFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAAV-CAG-tTA

    Plasmid

    #193338

    Purpose

    AAV-mediated expression of tTA under the CAG promoter in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

    Insert

    tTA2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. px330-UFL1 sgRNA2

    Plasmid

    #134636

    Purpose

    contains sgRNA targeting human UFL1 for gene knockout

    Depositor

    Yihong Ye

    Article

    Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

    Insert

    UFL1 sgRNA2 (UFL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pdUA-lacIN

    Plasmid

    #141079

    Purpose

    Plasmid vector for testing DNA binding proteins by the I-Block assay.

    Depositor

    Antal Kiss

    Article

    Szentes et al Nucleic Acids Res. 2020 Mar 18;48(5):e28

    Type

    Empty backbone

    Use

    Vector for the i-block assay designed to study dn…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Escherichia coli lacIN promoter

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. px330-UFL1 sgRNA1

    Plasmid

    #134635

    Purpose

    contains sgRNA targeting human UFL1 for gene knockout

    Depositor

    Yihong Ye

    Article

    Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

    Insert

    UFL1 sgRNA1 (UFL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pHH29_32 series plasmid

    Plasmid

    #120901

    Purpose

    Also known as pHH29_32_HG27, one of the pHH29_32 series plasmids. The component plasmid of a unregulated or a regulated device

    Depositor

    Domitilla Del Vecchio

    Article

    Huang et al Nat Commun. 2018 Dec 21;9(1):5415. doi:

    Inserts

    Ec-TTL-P109
    sRNA-A targeting sequence
    RBS TIR=974 of GFPop1 gene
    GFPop1
    RBS TIR=6474 of ECF32mut gene
    ECF32mut
    T7 terminator
    resource competitor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAAV-CRY2-CreN

    Plasmid

    #75268

    Purpose

    The second half of the photo-activatable cre recombinase

    Depositor

    Michael Bruchas

    Article

    Schindler et al Sci Rep. 2015 Sep 9;5:13627. doi: 10.103

    Insert

    second half of the cre recombinase fused to CRY2 (cryptochrome 2) that has absorbed blue light (Kennedy et al., 2010)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3.2/V5 hsa-mir-125

    Plasmid

    #26307

    Depositor

    David Bartel

    Article

    Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

    Insert

    hsa-mir-125 (MIR125A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

38. pSI-359

    Plasmid

    #131131

    Purpose

    gRNA expression vector for A-to-G base editing reporter

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    A-to-G reporter activation gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 promoter

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pTY153 EGFP-T2A-ORF6 SARS-CoV-2

    Plasmid

    #204975

    Purpose

    Co-express EGFP and SARS-CoV-2 ORF6

    Depositor

    Tim Mitchison

    Article

    Yoo et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

    Insert

    Open Reading Frame 6 (ORF6 SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pTY152 EGFP-T2A-ORF6 SARS-CoV-1

    Plasmid

    #204974

    Purpose

    Co-express EGFP and SARS-CoV-1 ORF6

    Depositor

    Tim Mitchison

    Article

    Yoo et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

    Insert

    Open Reading Frame 6 (ORF6 SARS-CoV-1)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. NET33 pEGFP-N2 (597)

    Plasmid

    #61989

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    NET33 (SCARA5 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCLXEBR-pTF-hM3Dq

    Plasmid

    #114283

    Purpose

    TET-inducible expression of the hM3Dq protein

    Depositor

    Patrick Salmon

    Article

    Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

    Insert

    hM3Dq
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pLenLox_U6 Nlgn2

    Plasmid

    #59358

    Purpose

    Lentiviral expression vector: Neuroligin 2 shRNA under control of mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor expression.

    Depositor

    Peter Scheiffele

    Article

    Chih et al Science. 2005 Feb 25;307(5713):1324-8. E

    Inserts

    Neuroligin 2 shRNA (rat and mouse) (Nlgn2, Nlgn2 Rat, Mouse)
    eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and mouse U6

    Available since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pGBW-m4046810

    Plasmid

    #149251

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 N (nucleocapsid)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 N (nucleocapsid) (N Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    May 11, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

45. pSI-535

    Plasmid

    #131130

    Purpose

    gRNA expression vector for C-to-T base editing reporter

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    C-to-T reporter activation gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 promoter

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pCZGY1584 Pcol-19::ebp-2::mGFP::unc-54 3'UTR

    Plasmid

    #129774

    Purpose

    Marker of microtubule dynamics in C. elegans hypodermis

    Depositor

    Andrew Chisholm

    Article

    Chuang et al Elife. 2016 Sep 23;5. doi: 10.7554/eLife

    Inserts

    Pcol-19
    ebp-2 (ebp-2 Nematode)
    unc-54 3'UTR
    

    Use

    Synthetic Biology; Gateway

    Tags

    Synthetic Intron and mGFP

    Expression

    Worm

    Mutation

    Promoter

    Pcol-19

    Available since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pTAL-MajSat-FLAG-NLS-VP64

    Plasmid

    #69074

    Purpose

    Expresses TALE for mouse major satellite with VP64 and FLAG under CAG promoter

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Ishiuchi et al Nat Struct Mol Biol. 2015 Aug 3. doi: 10

    Insert

    TALE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pOP459

    Plasmid

    #85933

    Purpose

    Encodes the Anti-Ebola 2G4 monoclonal antibody. Chimeric - mouse variable regions, human constant regions.

    Depositor

    Timothy Lu

    Article

    Purcell et al ACS Synth Biol. 2017 Aug 17. doi: 10.102

    Insert

    Anti-Ebola 2G4 monoclonal antibody
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pcDNA3.2/V5 hsa-mir-142

    Plasmid

    #26309

    Depositor

    David Bartel

    Article

    Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

    Insert

    hsa-mir-142 (MIR142 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

50. pcDNA3.2/V5 mmu-mir-223

    Plasmid

    #26334

    Depositor

    David Bartel

    Article

    Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

    Insert

    mmu-mir-223 (Mir223 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only