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pCAGGS_AKAR5’

Plasmid

#114141

Purpose

Mammalian expression of the AKAR5’ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

AKAR5'

Expression

Mammalian

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pJAF210

Plasmid

#52406

Purpose

Expresses Flag-tagged mouse Arf5

Depositor

Gregory Pazour

Article

Follit et al PLoS Genet. 2014 Feb 20;10(2):e1004170.

Insert

Arf5 (Arf5 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
pJAF214

Plasmid

#52404

Purpose

Expresses Flag-tagged mouse Arf3

Depositor

Gregory Pazour

Article

Follit et al PLoS Genet. 2014 Feb 20;10(2):e1004170.

Insert

Arf3 (Arf3 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
PCUP1-GFP-PHO8 K(24,25,30,33)R

Plasmid

#207044

Purpose

Expression of GFP-Pho8 point mutant under CUP1 promoter.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

PHO8

Tags

GFP

Expression

Yeast

Mutation

K(24,25,30,33)R

Promoter

pCUP1

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pmScarlet LRRK1 D1409A

Plasmid

#241257

Purpose

For exogenous expression of LRRK1 D1409A as an N-terminal fusion to mScarlet in mammalian cells

Depositor

Pietro De Camilli

Article

Hanna et al EMBO J (2025) 44: 6168 - 6195

Insert

LRRK1 D1409A (LRRK1 Human)

Tags

mScarlet

Expression

Mammalian

Mutation

D1409A

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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BS-ZJLEU-PTEF1-HFL1-D384A Y387A-GFP

Plasmid

#207024

Purpose

Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

HFL1

Tags

GFP

Expression

Yeast

Mutation

D384A Y387A

Promoter

pTEF1

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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BS-ZJLEU-PTEF1-HFL1-W371A I375A-GFP

Plasmid

#207023

Purpose

Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

HFL1

Tags

GFP

Expression

Yeast

Mutation

W371A I375A

Promoter

pTEF1

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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BS-ZJLEU-PTEF1-HFL1-Y387A-GFP

Plasmid

#207022

Purpose

Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

HFL1

Tags

GFP

Expression

Yeast

Mutation

Y387A

Promoter

pTEF1

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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BS-ZJLEU-PTEF1-HFL1-D384A-GFP

Plasmid

#207021

Purpose

Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

HFL1

Tags

GFP

Expression

Yeast

Mutation

D384A

Promoter

pTEF1

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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BS-ZJLEU-PTEF1-HFL1-I375A-GFP

Plasmid

#207020

Purpose

Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

HFL1

Tags

GFP

Expression

Yeast

Mutation

I375A

Promoter

pTEF1

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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BS-ZJLEU-PTEF1-HFL1-W371A-GFP

Plasmid

#207019

Purpose

Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

HFL1

Tags

GFP

Expression

Yeast

Mutation

W371A

Promoter

pTEF1

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-P1K-3VSV-Atg8-MET15

Plasmid

#207055

Purpose

Expression of 3VSV-Atg8. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Tags

3xVSV

Expression

Yeast

Promoter

pATG8

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pYea5.1_Notch1-L24_sfGFP

Plasmid

#207865

Purpose

Yeast 2µ vector for expression of Suc2SP-Notch1-L24_100-sfGFP-GAL4

Depositor

Dieter Langosch

Article

Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

Insert

Suc2SP-Notch1-L24_100-sfGFP-GAL4 fusion protein

Tags

Strep-TagII

Expression

Bacterial and Yeast

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pYea5.1_Notch1_sfGFP

Plasmid

#207801

Purpose

Yeast 2µ vector for expression of Suc2SP-Notch1_100-sfGFP-GAL4

Depositor

Dieter Langosch

Article

Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

Insert

Suc2SP-Notch1_100-sfGFP-GAL4 fusion protein

Tags

Strep-TagII

Expression

Bacterial and Yeast

Promoter

TEF

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pSEVA424::tsi5

Plasmid

#192960

Purpose

Expresses the protein Tsi51-76 for biological function studies in a broad-spectrum of organisms

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

tsi5

Expression

Bacterial

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pET29a(+)::D1141A

Plasmid

#192963

Purpose

Test the activity of the putative aspartyl protease motif DPXGL-(18)-DPXGL

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

Tse5_D1141A

Tags

9xhis + TEV tag

Expression

Bacterial

Mutation

D1141A

Available Since

Dec. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pET29a(+)::9xhis-Tse5

Plasmid

#192962

Purpose

Heterologous expression of Tse5 in E. coli Lemo21 cells for purification of Tse5-CT

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

Tse5

Tags

9xhis + TEV tag

Expression

Bacterial

Available Since

Dec. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pCB6-Occludin

Plasmid

#136637

Purpose

Expression of Occludin in mammalian cells

Depositor

Maria Balda, Karl Matter

Article

Balda et al J Cell Biol. 1996 Aug;134(4):1031-49. do

Insert

Occludin (OCLN Chicken)

Expression

Mammalian

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCB6-HAOccludin

Plasmid

#136638

Purpose

Expression of HAOccludin in mammalian cells

Depositor

Maria Balda, Karl Matter

Article

Balda et al J Cell Biol. 1996 Aug;134(4):1031-49. do

Insert

HAOccludin (OCLN Chicken)

Tags

HA (MQDLPGNDNSTAG)

Expression

Mammalian

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCB6-OccludinCT3

Plasmid

#136639

Purpose

Expression of OccludinCT3 in mammalian cells

Depositor

Maria Balda, Karl Matter

Article

Balda et al J Cell Biol. 1996 Aug;134(4):1031-49. do

Insert

OccludinCT3 (OCLN Chicken)

Expression

Mammalian

Mutation

Deletion of C-terminal cytoplasid domain

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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