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pCMV_SEPT9_i1-i5-msfGFP

Plasmid

#180332

Purpose

mammalian expression of human SEPT9_i1-i5 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v7 (SEPTIN9 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1-i5

Promoter

CMV

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-Bin1b

Plasmid

#176014

Purpose

Mammalian expression of zebrafish EGFP-Bin1b. Parton lab clone HYG

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Bin1b (bin1b Zebrafish)

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLAP-MIS12-KARD

Plasmid

#114057

Purpose

transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

Depositor

Geert Kops

Article

Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

Insert

MIS12-KARD (BUB1B Human)

Tags

YFP-TEV-S- Human LAP

Expression

Mammalian

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pN1_FKBP-HsKIF1C(886-1103)-mCh

Plasmid

#253322

Purpose

mammalian expression of the IDR domain (aa 886-1103) of HsKIF1C tagged with FKBP and mCherry. The FKBP tag enables rapamycin-induced attachment to FRB-tagged proteins.

Depositor

Kristen Verhey

Article

Geng et al J Cell Biol. 2025 Oct 6;224(10):e2025010

Insert

HsKIF1C(886-1103) (KIF1C Human)

Tags

FKBP and mCherry

Expression

Mammalian

Available Since

March 30, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pN1_FKBP-mCh-FTH1

Plasmid

#253323

Purpose

mammalian expression of human FTH1 tagged with FKBP and mCherry. FTH1 self-assembles into a 24-merparticle. FKBP enables rapamycin-induced attachment to FRB-tagged proteins.

Depositor

Kristen Verhey

Article

Geng et al J Cell Biol. 2025 Oct 6;224(10):e2025010

Insert

HsFTH1 (FTH1 Human)

Tags

FKBP, mCherry

Expression

Mammalian

Available Since

March 30, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pN1_FKBP-HsKIF1C(349-1103)-mCh

Plasmid

#253321

Purpose

mammalian expression of the stalk+tail domains (aa 349-1103) of HsKIF1C tagged with FKBP and mCherry. The FKBP tag enables rapamycin-induced attachment to FRB-tagged proteins.

Depositor

Kristen Verhey

Article

Geng et al J Cell Biol. 2025 Oct 6;224(10):e2025010

Insert

HsKIF1C(349-1103) (KIF1C Human)

Tags

FKBP and mCherry

Expression

Mammalian

Available Since

March 30, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pN1_HsKIF1C(1-376)-NC-LZ-TagBFP-FRB

Plasmid

#253326

Purpose

mammalian expression of the motor domain of human KIF1C tagged with TagBFP and FRB. NC and LZ facilitate stable dimerization. FRB enables rapamycin-induced attachment to FKBP-tagged proteins.

Depositor

Kristen Verhey

Article

Geng et al J Cell Biol. 2025 Oct 6;224(10):e2025010

Insert

HsKIF1C(1-376) (KIF1C Human)

Tags

TagBFP, FRB

Expression

Mammalian

Available Since

March 30, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pHNF4A-Enh_Gsta2-IRES2-H2BeGFP

Plasmid

#247049

Purpose

This plasmid was employed to generate the mouse model used in this study. It contains the human HNF4A gene enhancer linked to the Shh mini-promoter and the Gsta2-IRES2-H2BeGFP. Homologous arm

Depositor

Andrew McMahon

Article

Cheng et al J Am Soc Nephrol. 2025 Aug 21. doi: 10.1

Insert

Glutathione S-Transferase Alpha 2 (Gsta2 Mouse)

Expression

Mammalian

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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2xmyc-RAB10 (T73A)

Plasmid

#244984

Purpose

Express RAB10 in mammalian cells with 2xmyc tag and T73A mutation

Depositor

Luis Bonet-Ponce

Article

Bonet-Ponce et al J Cell Biol. 2025 Nov 3;224(11):e2024040

Insert

RAB10 (RAB10 Human)

Tags

2xmyc

Expression

Mammalian

Mutation

T73A

Promoter

CMV

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-mCGN-mCGNL1-Rod1-Myc

Plasmid

#236526

Purpose

For expression of chimeric CGN containing the Rod1 domain from CGNL1 (mouse) in mammalian cells

Depositor

Sandra Citi

Article

Flinois et al J Cell Sci. 2024 Mar 1;137(5):jcs260745.

Insert

CGN-CGNL1 (Cgn Mouse)

Tags

GFP and Myc

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GABARAP(G116A)-mCherry-GABARAP

Plasmid

#212113

Purpose

Expression of GABARAP split tandem protein with mCherry

Depositor

Dieter Willbold

Article

Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

Insert

GABARAP(G116A)-mCherry-GABARAP (GABARAP Discosoma sp., Human)

Tags

mCherry

Expression

Mammalian

Mutation

G116A (lipidation deficiency) of upstream GABARAP

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO mTagBFP2-GABARAP(G116A)-GABARAP

Plasmid

#212109

Purpose

Expression of N-terminally tagged GABARAP tandem construct with mTagBFP2

Depositor

Dieter Willbold

Article

Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

Insert

mTagBFP2-GABARAP(G116A)-GABARAP (GABARAP Human, Entacmaea quadricolor)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-rox-inv[Talpha1-iCre-pA]-rox-LynGFP

Plasmid

#196874

Purpose

Neuron-specific expression of LynGFP reporter. Used in combination with Talpha1-Dre-pA plasmids

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

LynGFP

Use

Cre/Lox; Dre/rox

Expression

Mammalian

Promoter

Quimeric CAG

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-lox-inv[Talpha1-Dre-pA]-lox-Lyn-EGFP

Plasmid

#196876

Purpose

Neuron-specific expression of LynGFP reporter. Used in combination with Talpha1-iCre-pA plasmids

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

LynEGFP

Use

Cre/Lox; Dre/rox

Expression

Mammalian

Promoter

Quimeric CAG

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-DIO-inv[roxSTOProx-ZsGreen-bGH]

Plasmid

#196883

Purpose

Dual Cre/Dre-dependent expression of ZsGreen. Used as Cre-Dre cotransfection reporter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

roxSTOProx-ZsGreen-bGH

Use

Cre/Lox; Dre/rox

Expression

Mammalian

Promoter

Quimeric CAG

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-DIO-inv[roxSTOProx-Lyn-GFP-bGH]

Plasmid

#196884

Purpose

Dual Cre/Dre-dependent expression of LynGFP. Used as Cre-Dre cotransfection reporter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

roxSTOProx-Lyn-GFP-bGH

Use

Cre/Lox; Dre/rox

Expression

Mammalian

Promoter

Quimeric CAG

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pBactin-DIO-inv[roxSTOProx-ZsGreen-bGH]

Plasmid

#196886

Purpose

Dual Cre/Dre-dependent expression of ZsGreen. Used as Cre-Dre cotransfection reporter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

roxSTOProx-ZsGreen-bGH

Use

Cre/Lox; Dre/rox

Expression

Mammalian

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-FKBP-Halo-Dync1h1motor

Plasmid

#191333

Purpose

Expresses the fluorescently labeled dynein motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FKBP-Halo-Dync1h1motor (Dync1h1 Mouse)

Expression

Mammalian

Mutation

DYNC1H1 motor domain aa 1453-4644

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-FKBP-Dync1h1motor

Plasmid

#191332

Purpose

Expresses the dynein motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FKBP-Dync1h1motor (Dync1h1 Mouse)

Expression

Mammalian

Mutation

DYNC1H1 motor domain aa 1453-4644

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 S111F-msfGFP

Plasmid

#180340

Purpose

mammalian expression of human SEPT9_i1 S111F fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 S111F

Promoter

CMV

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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