We narrowed to 4,028 results for: pCMV mammalian

1. pCMV HA hRb delta CDK + S230

   Plasmid

   #58907

   Purpose

   mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S230 restored

   Depositor

   Steven Dowdy

   Article

   Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

   Insert

   Rb (RB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delta CDK* with S230 restored

   Promoter

   CMV

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pCMV HA hRB delta CDK + T252

   Plasmid

   #58910

   Purpose

   mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T252 restored

   Depositor

   Steven Dowdy

   Article

   Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

   Insert

   Rb (RB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delta CDK* with T252 restored

   Promoter

   CMV

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pCMV-DNAJC5-ΔC30 (Δ169-198)

   Plasmid

   #205722

   Purpose

   Mammalian expression of DNAJC5-ΔC30 (Δ169-198)

   Depositor

   Randy Schekman

   Article

   Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

   Insert

   DNAJC5 (DNAJC5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Δ169-198

   Promoter

   Available since

   Feb. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-XPACK-DNAJC5 (L115R)- HaloTag

   Plasmid

   #205712

   Purpose

   Mammalian expression of XPACK-DNAJC5 (L115R)-HaloTag

   Depositor

   Randy Schekman

   Article

   Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

   Insert

   DNAJC5 (DNAJC5 Human)
   

   Use

   Tags

   HaloTag and XPACK (GCINSKRKD)

   Expression

   Mammalian

   Mutation

   L115R

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-DEAD XPACK-DNAJC5 (L115R)

   Plasmid

   #205718

   Purpose

   Mammalian expression of DEAD XPACK-DNAJC5 (L115R)

   Depositor

   Randy Schekman

   Article

   Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

   Insert

   DNAJC5 (DNAJC5 Human)
   

   Use

   Tags

   Dead XPACK (GCINIKRKD)

   Expression

   Mammalian

   Mutation

   L115R

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV-DNAJC5-ΔC10 (Δ189-198)

   Plasmid

   #205720

   Purpose

   Mammalian expression of DNAJC5-ΔC10 (Δ189-198)

   Depositor

   Randy Schekman

   Article

   Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

   Insert

   DNAJC5 (DNAJC5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Δ189-198

   Promoter

   Available since

   Jan. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-DNAJC5-ΔC20 (Δ179-198)

   Plasmid

   #205721

   Purpose

   Mammalian expression of DNAJC5-ΔC20 (Δ179-198)

   Depositor

   Randy Schekman

   Article

   Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

   Insert

   DNAJC5 (DNAJC5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Δ179-198

   Promoter

   Available since

   Jan. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-DNAJC5-ΔC40 (Δ151-198)

   Plasmid

   #205723

   Purpose

   Mammalian expression of DNAJC5-ΔC40 (Δ151-198)

   Depositor

   Randy Schekman

   Article

   Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

   Insert

   DNAJC5 (DNAJC5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Δ151-198

   Promoter

   Available since

   Jan. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV WS Full Myc (Wrn cDNA)

   Plasmid

   #19273

   Depositor

   Philip Leder

   Insert

   mouse Wrn cDNA (Wrn Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-T7-SpCas9-NG-P2A-EGFP (RTW3677)

    Plasmid

    #139994

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-T7-SpG-P2A-EGFP (RTW4177)

    Plasmid

    #139988

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 variant named SpG with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV6-XL4 ASXL1 (1-479) 3x FLAG

    Plasmid

    #74262

    Purpose

    Cancer-associated ASXL1 truncation mutant in mammalian expression vector

    Depositor

    Anjana Rao

    Article

    Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

    Insert

    ASXL1 (ASXL1 Human)
    

    Use

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    Contains ASXL1 amino acids 1-479

    Promoter

    5’LTR

    Available since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

13. PCMV-intron myc Rab11 S25N

    Plasmid

    #46786

    Purpose

    expression of S25N Rab11a in mammalian cells

    Depositor

    Terry Hébert

    Article

    Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

    Insert

    RAB11A S25N (RAB11A Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    S25N dominant negative

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pCMV-Myr-HA-mIFITM3-C71,72,105A-CLVL

    Plasmid

    #58414

    Purpose

    Expresses murine IFITM3-C71,72,105A with an N-terminal myristoylation sequence from HIV Gag followed by an HA tag, as well as a C-terminal CaaX prenylation motif, in mammalian cells

    Depositor

    Howard Hang, Jacob Yount

    Article

    Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

    Insert

    IFITM3 (Ifitm3 Mouse)
    

    Use

    Tags

    HA internal

    Expression

    Mammalian

    Mutation

    Cysteines 71, 72, and 105 to Alanine; The sequenc…

    Promoter

    CMV IE

    Available since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pCMV6-AN-HA_aa 321-843 REV1

    Plasmid

    #201587

    Purpose

    Expresses the catalytic domain (aa 321-843) of human DNA repair protein REV1 with a HA tag in mammalian cells

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    DNA repair protein REV1 (REV1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Expresses amino acids 321-843 of DNA repair prote…

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV5D HA-L2D/V9D/I19D PPM1H

    Plasmid

    #207341

    Purpose

    Mammalian expression of HA-L2D/V9D/I19D PPM1H

    Depositor

    Suzanne Pfeffer

    Article

    Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

    Insert

    PPM1H (PPM1H Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    L2D/V9D/I19D

    Promoter

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-TAPtagN-PrP-SBP-HsAGO2_AC

    Plasmid

    #148440

    Purpose

    Mammalian Expression of HsAGO2

    Depositor

    Elisa Izaurralde

    Article

    Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

    Insert

    HsAGO2 (AGO2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one silent mutation C840Tand sequence differs in …

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-PEmax-P2A-hP53DD-P2A-hMLH1dn

    Plasmid

    #214102

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and P2A-human MLH1dn

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Insert

    PE-Plus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLD037-pCMV-APOBEC-Cas9(D10A)-rXRCC1

    Plasmid

    #165444

    Purpose

    Expresses ACX, rXRCC1 in mammalian cells

    Depositor

    Wei Leong Chew

    Article

    Chen et al Nat Commun. 2021 Mar 2;12(1):1384. doi:

    Insert

    APOBEC-nCas9-rXRCC1 (Apobec1 Rat, Synthetic, Streptococcus pyogenes)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV5B-Flag-Smurf1 C699A

    Plasmid

    #11753

    Depositor

    Jeff Wrana

    Article

    Zhu et al Nature. 1999 Aug 12. 400(6745):687-93.

    Insert

    SMURF1 (SMURF1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    C699A: catalytically inactive

    Promoter

    Available since

    May 12, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits