We narrowed to 3,880 results for: Kap

1. pCMV4-3 HA/IkB-alpha

   Plasmid

   #21985

   Depositor

   Warner Greene

   Article

   Sun et al Mol Cell Biol. 1996 Mar . 16(3):1058-65.

   Insert

   I-kappa B alpha (NFKBIA Human)
   

   Use

   Tags

   3x HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCM18

   Plasmid

   #78565

   Purpose

   60-copy, promoterless, oriE1(ColE1) luxCDABE, aph, hok-sok, par, parA, rrnB Stabilized luciferase-expressing plasmid

   Depositor

   James Kaper

   Article

   Morin et al FEMS Immunol Med Microbiol. 2009 Oct;57(

   Type

   Empty backbone

   Use

   Tags

   luxCDABE

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRK792

   Plasmid

   #8830

   Depositor

   David Waugh

   Article

   Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

   Insert

   TEV protease, S219P mutant
   

   Use

   Tags

   His, MBP (cleaved), and polyarginine

   Expression

   Bacterial

   Mutation

   S219P mutation

   Promoter

   Available since

   July 18, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. Ubc.IkBSR-Flag Pgk.Cre

   Plasmid

   #22503

   Depositor

   Tyler Jacks

   Article

   Meylan et al Nature. 2009 Nov 5;462(7269):104-7. doi:

   Insert

   IkBSR
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. TRE.IkBSR-Flag Pgk.Cre

   Plasmid

   #22504

   Depositor

   Tyler Jacks

   Article

   Meylan et al Nature. 2009 Nov 5;462(7269):104-7. doi:

   Insert

   IkBSR
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJK438

   Plasmid

   #71590

   Purpose

   Produces Thermoplasma acidophilum citrate synthase with C-terminal His6 tag (TpCSH6)

   Depositor

   T. J. Kappock

   Article

   Murphy et al Acta Crystallogr F Struct Biol Commun. 2

   Insert

   citrate synthase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJK240

   Plasmid

   #71127

   Purpose

   Expression of Acetobacter aceti 1023 citrate synthase (AaCS) in E. coli

   Depositor

   T. J. Kappock

   Article

   Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

   Insert

   citrate synthase
   

   Use

   Tags

   GyrA intein and His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCVD438

   Plasmid

   #63759

   Purpose

   eae gene (3476 bp) from pJY21 removed by SamI/SphI digestion, cloned into EcoRV/SphI sites of pACYC184. Insertional inactivation of tetracycline gene.

   Depositor

   James Kaper

   Article

   Jerse et al Proc Natl Acad Sci U S A. 1990 Oct;87(20

   Insert

   eae
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 6, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pJK667

   Plasmid

   #71690

   Purpose

   Produces E. coli acetate kinase with an N-terminal His6 tag (H6AckA)

   Depositor

   T. J. Kappock

   Article

   Murphy et al Front Chem. 2016 May 23;4:23. doi: 10.33

   Insert

   acetate kinase (ackA Escherichia coli)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   June 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJK371

    Plasmid

    #73596

    Purpose

    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-3) with C-terminal c-myc epitope

    Depositor

    T. J. Kappock

    Inserts

    delta-12 fatty acid acetylenase
    delta-12 fatty acid desaturase (form 3)
    

    Use

    Tags

    FLAG tag and c-myc tag

    Expression

    Yeast

    Mutation

    Point mutants in FAD2-3 ORF relative to deposited…

    Promoter

    GAL1 and GAL10

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJK273

    Plasmid

    #71563

    Purpose

    Produces Crepis alpina delta12 fatty acid desaturase (FAD2-2) in yeast

    Depositor

    T. J. Kappock

    Article

    Nam et al J Exp Bot. 2007;58(6):1421-32. Epub 2007

    Insert

    delta12 fatty acid desaturase (form 2)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJK277

    Plasmid

    #71565

    Purpose

    Produces Crepis alpina delta15 fatty acid desaturase (FAD3) in yeast

    Depositor

    T. J. Kappock

    Article

    Nam et al J Exp Bot. 2007;58(6):1421-32. Epub 2007

    Insert

    delta15 fatty acid desaturase
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJK571

    Plasmid

    #75379

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits and associated hypothetical protein with N-terminal His6 tag (AaPurS, H6AaOrfY, AaPurQ, AaPurL-G209D)

    Depositor

    T. J. Kappock

    Inserts

    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Use

    Gene synthesis

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes glycine-209 to aspartate and synthetic ge…

    Promoter

    n/a

    Available since

    Aug. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJK598

    Plasmid

    #72523

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits, one with C-terminal His6 tag, and associated hypothetical protein (AaPurS, AaOrfY, AaPurQ, AaPurL)

    Depositor

    T. J. Kappock

    Inserts

    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    synthetic gene

    Promoter

    T7 and n/a

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJK560

    Plasmid

    #71585

    Purpose

    Produces E. coli acetyl-CoA:oxalate CoA-transferase (yfdE) with Arg232>His mutant (YfdE-R232H)

    Depositor

    T. J. Kappock

    Article

    Mullins et al PLoS One. 2013 Jul 23;8(7):e67901. doi:

    Insert

    acetyl-CoA:oxalate CoA-transferase (yfdE )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changed arginine-232 to histidine

    Promoter

    T7 promoter

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJK504

    Plasmid

    #71584

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase (AarC) with C-terminal His6 tag and Cys357>Tyr mutant (AarCH6-C357Y)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Acetic Acid Bacteria. 2(s1):e3 (2013)

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes cysteine-357 to tyrosine

    Promoter

    T7

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJK612

    Plasmid

    #72533

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits; associated hypothetical protein N-terminal domain (AaPurS, AaOrfY[1-69], AaPurQ, AaPurL)

    Depositor

    T. J. Kappock

    Inserts

    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    residues 1-69 only; C-terminal domain removed and…

    Promoter

    T7 and n/a

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJK613

    Plasmid

    #72534

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits, one with His6 tag; associated hypothetical protein N-terminal domain (AaPurS, AaOrfY[1-69], AaPurQ, AaPurLH6)

    Depositor

    T. J. Kappock

    Inserts

    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    residues 1-69 only; C-terminal domain removed and…

    Promoter

    T7 and n/a

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJK619

    Plasmid

    #72536

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit, N-term His-tag; hypothet. protein; glutaminase subunit; large subunit (H6AaPurS, AaOrfY, AaPurQ, AaPurL)

    Depositor

    T. J. Kappock

    Inserts

    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    synthetic gene

    Promoter

    T7 and n/a

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJK324

    Plasmid

    #71838

    Purpose

    Cloned region contains Acetobacter aceti 1023 purE gene

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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