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pWUKI Cas1 Cas2 T72I

Plasmid

#80100

Purpose

Encodes Cas1 and Cas2 T72I

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array

Expression

Bacterial

Mutation

T72I

Availability

Academic Institutions and Nonprofits only

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pWUKI Cas1 Cas2 G74A

Plasmid

#80101

Purpose

Encodes Cas1 and Cas2 G74A

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array

Expression

Bacterial

Mutation

G74A

Availability

Academic Institutions and Nonprofits only

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fIM1264

Plasmid

#132571

Purpose

pZA2-pLlacO-oxyR

Depositor

Timothy Lu

Article

Muller et al Nat Commun. 2019 Sep 6;10(1):4028. doi:

Insert

pLlacO-oxyR-TermT1 (oxyR )

Expression

Bacterial

Promoter

pLlacO

Availability

Academic Institutions and Nonprofits only

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pIM23

Plasmid

#132586

Purpose

pZA4-oxySp-mCherry-TEVrs-LAA

Depositor

Timothy Lu

Article

Muller et al Nat Commun. 2019 Sep 6;10(1):4028. doi:

Inserts

oxySp-mCherry-TEVrs-LAA-TermT1
None

Expression

Bacterial

Promoter

none and oxySp

Availability

Academic Institutions and Nonprofits only

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ATMY-C321

Bacterial Strain

#218774

Purpose

E. coli strain, genotype: C321.ΔA pUltraBR-MjY ΔtyrS tyrTV::tolC tyrU::GentR lambda::ZeoR

Depositor

Abhishek Chatterjee

Article

Ficaretta et al ACS Chem Biol. 2024 Jun 24. doi: 10.1021

Bacterial Resistance

Spectinomycin (50 ug/mL), Gentamycin (10 ug/mL)

Species

Escherichia coli

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only
E. coli BW25113 ΔtnaA ΔtrpR

Bacterial Strain

#205015

Purpose

Used for the characterization of the TrpR1-PtrpO1 biosensor system with the deletion of tnaA gene and trpR gene (no antibiotics marker)

Depositor

Yajun Yan

Article

Gong et al ACS Synth Biol. 2022 Jun 17;11(6):2175-2

Bacterial Resistance

None

Species

Escherichia coli

Available Since

Aug. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits
rEc.13.delA

Bacterial Strain

#69495

Purpose

Recoded strain from MG1655, prfA deleted

Depositor

Michael Jewett

Article

Hong et al ACS Synth Biol. 2014 Jun 20;3(6):398-409

Bacterial Resistance

Ampicillin

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only
TB205 △proC △trpR

Bacterial Strain

#229546

Purpose

Fluorescently labelled (mCherry) E. coli, auxotrophic for proline, tryptophan overproducer

Depositor

Yolanda Schaerli

Article

Pignon et al ISME J. 2025 Jun 13:wraf122. doi: 10.109

Bacterial Resistance

None

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only
TB204 △trpC proB74

Bacterial Strain

#229547

Purpose

Fluorescently labelled (sfGFP) E. coli, auxotrophic for tryptophan, proline overproducer

Depositor

Yolanda Schaerli

Article

Pignon et al ISME J. 2025 Jun 13:wraf122. doi: 10.109

Bacterial Resistance

None

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only
TB204 △hisD proB74

Bacterial Strain

#229550

Purpose

Fluorescently labelled (sfGFP) E. coli, auxotrophic for histidine, proline overproducer

Depositor

Yolanda Schaerli

Article

Pignon et al ISME J. 2025 Jun 13:wraf122. doi: 10.109

Bacterial Resistance

None

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only
TB204 △metB proB74

Bacterial Strain

#229551

Purpose

Fluorescently labelled (sfGFP) E. coli, auxotrophic for methionine, proline overproducer

Depositor

Yolanda Schaerli

Article

Pignon et al ISME J. 2025 Jun 13:wraf122. doi: 10.109

Bacterial Resistance

None

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only
TB205 △proC △hisL

Bacterial Strain

#229552

Purpose

Fluorescently labelled (mCherry) E. coli, auxotrophic for proline, histidine overproducer

Depositor

Yolanda Schaerli

Article

Pignon et al ISME J. 2025 Jun 13:wraf122. doi: 10.109

Bacterial Resistance

None

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only
pGIPZ EF1a myc tagged mbIL2 IRES miRFP720-P2A-Blast

Plasmid

#248031

Purpose

Constitutively expresses myc tagged membrane bound IL2, miRFP720 and a blasticidin resistance gene in human cells

Depositor

Joshua Leonard

Article

Deol et al GEN Biotechnol. 2023 Jun 1;2(3):228-246.

Insert

myc-tagged membrane bound IL2 (IL2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

human EF1a

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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His-PHD2-181-426

Plasmid

#223551

Purpose

His-tagged human PHD2 catalytic domain with TEV cleavage site between His and PHD2 for PHD2 purification

Depositor

Somshuvra Mukhopadhyay

Article

Gurol et al Proc Natl Acad Sci U S A. 2024 Jun 25;12

Insert

PHD2 (EGLN1 Human)

Tags

6x His

Expression

Bacterial

Mutation

Deleted amino acids 1-180.

Promoter

trc

Available Since

Sept. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGIPZ EF1a myc tagged mbIL15 IRES miRFP720-P2A-Blast

Plasmid

#248032

Purpose

Constitutively expresses myc tagged membrane bound IL15, miRFP720 and a blasticidin resistance gene in human cells

Depositor

Joshua Leonard

Article

Deol et al GEN Biotechnol. 2023 Jun 1;2(3):228-246.

Insert

myc-tagged membrane bound IL15 (IL15 Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Promoter

human EF1a

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA

Plasmid

#154867

Purpose

Flp-dependent hM4D(Gi) DREADD-mCherry

Depositor

Ulrik Gether

Article

Runegaard et al eNeuro. 2018 Jun 18;5(3). pii: eN-NWR-03

Has Service

AAV Retrograde and AAV8

Insert

hM4D(Gi)-mCherry

Use

AAV; Flp-frt

Tags

mCherry

Expression

Mammalian

Promoter

Human Synapsin-1

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pTMB352_ZFcharm_Kv1_split-inteins

Plasmid

#220848

Purpose

Lentiviral vector expressing ZFcharm Kv2 fused to ZF3 and MmZFP_81187 via split Npu inteins; perfect match ZF3 binding site located upstream of pEFS

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

ZFcharm Kv2

Use

Lentiviral

Tags

P2A-mCherry

Expression

Mammalian

Mutation

N/A

Promoter

EFS

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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CaMKAR

Plasmid

#205315

Purpose

Fluorescent Reporter for CaMKII Activity

Depositor

Mark Anderson, Elizabeth Luczak

Article

Reyes Gaido et al Sci Transl Med. 2023 Jun 21;15(701):eabq

Insert

CaMKAR

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFPc1 QFR IP3R3

Plasmid

#236454

Purpose

transient overexpression of IP3R3 in mammalian cells

Depositor

Michele Pagano

Article

Kuchay et al Nature. 2017 Jun 22;546(7659):554-558. d

Insert

IP3R3 (Itpr3 Rat)

Expression

Mammalian

Mutation

Q-FR/A-AA

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Nav1.6(WT, Y371C)-HA

Plasmid

#239765

Purpose

Expresses TTX-resistant (Y371C) variant of mouse Nav1.6WT with a C-terminal HA tag in mammalian cells.

Depositor

Ivana Nikić-Spiegel

Article

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Scn8a (Scn8a Mouse)

Tags

HA-tag

Expression

Mammalian

Mutation

Y371C

Promoter

CMV

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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