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Plasmid#109919PurposeProtein expression and purification of EEF1A2_GTP-EFTU-D2DepositorUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only
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pSBbi-Pur-Lyn-AktAR2-D
Plasmid#125198PurposeSB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted Lyn-AktAR2 variant of FRET-based AKT activity reporterDepositorInsertLyn-AktAR2-D
UseTransposonTagsLyn tag (GCIKSKRKD), mCerulean3 and cpVenus[E172]ExpressionMammalianMutationFoxO1 Thr-24 changed to Val rendering this inacti…PromoterEF1a/RPBSAAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLEX-307_LgBiT
Plasmid#191211PurposeLentiviral-mediated stable expression of LgBiT for slit nanoluciferase complementation assays in mammalian cellsDepositorInsertLgBiT (large bit)
UseLentiviral and LuciferaseTagsExpressionMutationAB030246.1 (mutated in PMID 26569370)PromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-Twstrep
Plasmid#208623Purposefor E. coli expression of C-ter Twin Strep tagged human RPL3 (ENST00000216146)DepositorInserthuman RPL3 (ENST00000216146) (RPL3 Human)
UseTags6xHis and Twin StrepExpressionBacterialMutationPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE (AAV1)
Viral Prep#105322-AAV1PurposeReady-to-use AAV1 particles produced from pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE (#105322). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE plasmid DNA. Synapsin-driven, Cre-dependent jGCaMP7c expression. jGCaMP7c exhibits high contrast between peak fluorescence and resting fluorescence. It is useful for activity imaging of large populations of densely-labeled neurons because background fluorescence from inactive neurons is reduced. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceJune 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDY1052 PASTEv3 pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT
Plasmid#179105PurposepCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINTDepositorInsertpCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDY0423 Cargo EGFP with AttP Bxb1 site for in-frame fusion (Parent minicircle)
Plasmid#179116PurposeCargo EGFP with AttP Bxb1 site for in-frame fusion (Parent minicircle)DepositorInsertCargo EGFP with AttP Bxb1 site for in-frame fusion
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDY0863 PASTEv4 pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT
Plasmid#179106PurposepCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINTDepositorInsertpCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDY0663 ACTB atgRNA with v2 scaffold
Plasmid#179108PurposeACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA with v2 scaffoldDepositorInsertACTB N-term PBS 13 RT 29 AttB 46 atgRNA with v2 scaffold
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDY0207 ACTB atgRNA with v1 scaffold
Plasmid#179107PurposeACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNADepositorInsertACTB N-term PBS 13 RT 29 AttB 46 atgRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV-LgBit
Plasmid#234738PurposeMammalian expression of large NanoBit fragment (LgBit)DepositorInsertLgBit
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
PSD-95-pTagRFP
Plasmid#52671Purposeexpression of TagRFP-tagged PSD-95DepositorInsertPSD-95 (Dlg4 Rat)
UseTagstagRFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKAR2-Br512g3.1
Plasmid#171200PurposeExpresses Br512g3.1 polymerase in E.coliDepositorInsertEngineered Bst DNA Polymerase I Large Fragment
UseTags8x His tag; Villin Headpiece 47 amino acid (vHP47…ExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDL444
Plasmid#29337DepositorInsertSmall form of HDV Delta Antigen
UseTagsExpressionMammalianMutationThe small delta antigen lacks the final 19 aa whi…PromoterAvailable sinceSept. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pKAR2-Br512g3.2
Plasmid#171201PurposeExpresses Br512g3.2 polymerase in E.coliDepositorInsertEngineered Bst DNA Polymerase I Large Fragment
UseTags8x His tag; Villin Headpiece 47 amino acid (vHP47…ExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-IV-3xFLAG/HA/NBL10
Plasmid#110568PurposeCre-dependently expresses large ribosomal subunit protein RPL10A with a 3xFLAG/HA tagDepositorInsert3xFLAG/HA-tagged anti-GFP VHH
UseAAV, Cre/Lox, and Synthetic BiologyTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
PSD-95_Y12E-pTagRFP
Plasmid#52672Purposeexpression of TagRFP-tagged PSD-95_Y12EDepositorInsertPSD-95 (Dlg4 Rat)
UseTagstagRFPExpressionMammalianMutationY12EPromoterCMVAvailable sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pAdEasy-2
Plasmid#16401PurposeAdenoviral vector for use in AdEasy System, for use with large inserts.DepositorTypeEmpty backboneUseAdenoviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
p5sheLL
Plasmid#46953Purposeencode HPV5 L1 and L2, Bicistronic, too large to self-packageDepositorInsertHPV5L1+L2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 19, 2013AvailabilityAcademic Institutions and Nonprofits only