We narrowed to 7,766 results for: loxP

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MYH11-NanoLuc-2A-tdTomato

Plasmid

#132569

Purpose

Donor vector to knockin NanoLuc and tdTomado (separated by 2A) into MYH11 allele

Depositor

James Thomson

Publication

Zhang et al Stem Cell Reports. 2019 Jun 11;12(6):126

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Human MYH11 knockin homology arms (MYH11 Human)

Expression

Mammalian

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-CAG-FLEX-jGCaMP7c variant 1513-WPRE

Plasmid

#105323

Purpose

AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre-dependent expression

Depositor

Douglas Kim, GENIE Project

Publication

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Insert

jGCaMP7c variant 1513

Use

AAV and Cre/Lox

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Promoter

CAG

Available since

Jan. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pEx1-pEF-H2B-mCherry-T2A-rTetR-KRAB-Zeo

Plasmid

#78352

Purpose

Expresses rTetR-KRAB for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

Depositor

Michael Elowitz

Publication

Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

H2B-mCherry-T2A-rTetR-KRAB

Use

Synthetic Biology

Tags

rTetR

Expression

Mammalian

Promoter

pEF alpha

Available since

July 7, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pFp10

Plasmid

#170460

Purpose

EarlyBac insect cell expression system

Depositor

Hiro Furukawa

Publication

Chou et al Cell. 2020 Jul 23;182(2):357-371.e13. do

Insert

none

Expression

Insect

Promoter

dHsp70

Available since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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pXP416

Plasmid

#26842

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Publication

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Expression

Yeast

Available since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-mCherry-LATeNT

Plasmid

#240992

Purpose

Cre-dependent expression of LATeNT

Depositor

Alice Ting

Publication

Roh et al bioRxiv 2025.01.27.635161; doi: 10.1101/

Insert

mCherry-V5-LATeNT

Use

AAV

Tags

V5 (internal)

Expression

Mammalian

Available since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-GRAB_eCB2.0

Plasmid

#164606

Purpose

Express the endocannabinoid sensor GRAB_eCB2.0 in Cre positive neurons

Depositor

Yulong Li

Publication

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid sensor GRAB_eCB2.0

Use

AAV

Mutation

S383T

Promoter

hSyn

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-TRE-DIO-mNeonGreen

Plasmid

#99132

Purpose

An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein mNeonGreen

Depositor

Viviana Gradinaru

Publication

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

DIO-mNeonGreen

Use

AAV

Expression

Mammalian

Promoter

TRE

Available since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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pBIG1c

Plasmid

#80613

Purpose

pBIG1c - Step1 vector c (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Publication

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pBIG2abcd

Plasmid

#80618

Purpose

pBIG2abcd - Step2 vector abcd (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Publication

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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scaffold-linker-U6

Plasmid

#89639

Purpose

construction of paired sgRNAs driven by two U6 promoters. Vector itself only has one U6 promoter.

Depositor

Wensheng Wei

Publication

Zhu et al Nat Biotechnol. 2016 Dec;34(12):1279-128

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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Tol2-CAG::Nucbow

Plasmid

#158992

Purpose

Multicolor nuclear-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing H2B-tethered mEYFP, mCherry or mCerulean upon Cre recombination)

Depositor

Jean Livet

Publication

Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10

Insert

Nucbow

Tags

All FPs are fused to H2B

Expression

Mammalian

Promoter

CAG

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p28BIOH-LIC

Plasmid

#62352

Purpose

E. coli expression vector with N-terminal AviTag and a C-terminal Hexa-HIS tag

Depositor

Cheryl Arrowsmith

Type

Empty backbone

Tags

6xHis and AviTag

Expression

Bacterial

Promoter

T7-lacO

Available since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDS272

Plasmid

#34975

Depositor

Michael Glotzer

Publication

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2-GFP-LOVpep (MID2 Budding Yeast, A. sativa)

Expression

Yeast

Mutation

T406A, T407A

Promoter

TEF

Available since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-pAceR-Kv2.1PR

Plasmid

#195534

Purpose

Cre-dependent red fluorescent, positive response-polarity voltage indicator; soma-targeted

Depositor

Vincent Pieribone

Publication

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

pAceR-Kv2.1 proximal restriction sequence

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

VARNAM 78E, 81D, 92N; SI linker

Promoter

CAG

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pJT050 lenti pEF-rTetR-LibCloneSite-T2A-mCherry-BSD-WPRE

Plasmid

#161925

Purpose

Lentiviral backbone for GoldenGate cloning effectors as a fusion with rTetR

Depositor

Michael Bassik, Lacra Bintu

Publication

Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

Type

Empty backbone

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

pEF

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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LZF1735 pAAV_hSyn-DiO-somArchon1-EGFP-P2A-somCheRiff

Plasmid

#126512

Purpose

Optopatch4. Simultaneous optical recording and manipulation of electrical activity in neurons

Depositor

Adam Cohen

Publication

Fan et al Cell. 2020 Jan 23. pii: S0092-8674(20)30

Insert

somArchon1-EGFP-P2A-somCheRiff

Use

AAV

Expression

Mammalian

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pHA1

Plasmid

#85709

Purpose

This plasmid provides a BAC vector backbone, which can be used for cloning of BAC inserts, for instance, herpesvirus genomes

Depositor

Ulrich Koszinowski, Martin Messerle

Publication

Adler et al J Virol. 2000 Aug;74(15):6964-74.

Type

Empty backbone

Use

Cre/Lox; Herpesvirus cloning

Expression

Bacterial

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pJW1583

Plasmid

#121054

Purpose

GFP^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.

Depositor

Jordan Ward

Publication

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

GFP^SEC^AID*::3xFlag

Use

CRISPR and Cre/Lox

Tags

3xFLAG, C. elegans codon optimized GFP, and auxin…

Expression

Worm

Available since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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EF1a_KLF1_P2A_Hygro_Barcode

Plasmid

#120488

Purpose

Barcoded lentiviral vector to express KLF1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Publication

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF1 (KLF1 Human)

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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