Addgene

We narrowed to 26,180 results for: sta

Showing: 1841 - 1860 of 26180 results

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pcDNA3-ApaLIE225A, K227A-HA

Plasmid

#211460

Purpose

Express mitochondrial localized ApaLI with E225A and K227A mutations

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

ApaLI-E225A-K227A

Use

Tags

HA

Expression

Mammalian

Mutation

E225A, K227A

Promoter

CMV

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pEF mito-PAcGFP

Plasmid

#188905

Purpose

Expresses mitochondrial photoactivatable GFP

Depositor

Edwin Chapman

Article

Vevea et al J Neurosci. 2023 May 10;43(19):3421-3438

Insert

mitochondrial photoactivatable GFP

Use

Tags

Mitochondrial leader sequence: MSVLTPLLLRGLTGSARR…

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pMVS184

Plasmid

#176294

Purpose

Reporter_ZIFUAS_minCMVpromoter_GFP_AAVS1_Puromycin

Depositor

Max Staller

Article

Staller et al Cell Syst. 2022 Apr 20;13(4):334-345.e5.

Insert

Promoter and GFP

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

4x Zinc finger binding sites and mini CMV promoter

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pQE80L-His-SpyTag-XMod-Doc(WT)

Plasmid

#157675

Purpose

Expression of XMod-Doc wild type with His6x tag and SpyTag.

Depositor

Michael Nash

Article

Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

Insert

XMod-Doc Wild type with Histag and SpyTag

Use

Tags

Histag and SpyTag

Expression

Bacterial

Mutation

Promoter

T5

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pQE80L-Coh-His-SpyTag

Plasmid

#157673

Purpose

Expression of Coh (type III from Ruminococcus flavefaciens) with His6x tag and SpyTag.

Depositor

Michael Nash

Article

Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

Insert

Cohesin (R.f) with Histag and SpyTag

Use

Tags

Histag and SpyTag

Expression

Bacterial

Mutation

Promoter

T5

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCVD402

Plasmid

#66148

Purpose

to make a probe to detect Heat stable enterotoxin gene (human)

Depositor

James Kaper

Article

Nishibuchi et al J Clin Microbiol. 1988 Apr;26(4):784-6.

Insert

STh

Use

Cloning vector

Tags

Expression

Mutation

Promoter

none

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
pCVD404

Plasmid

#66149

Purpose

to make a probe to detect Heat stable enterotoxin gene (porcine)

Depositor

James Kaper

Article

Nishibuchi et al J Clin Microbiol. 1988 Apr;26(4):784-6.

Insert

STp

Use

Cloning vector

Tags

Expression

Mutation

Promoter

none

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
pLVX-TetOne-Puro-GFP

Plasmid

#171123

Purpose

Doxycycline inducible expression of GFP

Depositor

Jason Sheltzer

Article

Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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delCMV-mCherry

Plasmid

#214143

Purpose

Control sensor

Depositor

Leif Dehmelt

Article

Nanda et al Nat Commun. 2023 Dec 15;14(1):8356. doi:

Insert

mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

delCMV

Available since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pHL-sec

Plasmid

#99845

Purpose

Mammalian expression with secretion signal sequence

Depositor

Edith Yvonne Jones

Article

Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

Insert

ephrin b2 (EFNB2 Human)

Use

Tags

His

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pMo130-TelR

Plasmid

#50799

Purpose

Suicide vector for generating chromosomal deletions in multidrug-resistant bacteria

Depositor

Kim Lee Chua

Article

Amin et al BMC Microbiol. 2013 Jul 13;13:158. doi:

Insert

tellurite-resistance cassette

Use

Suicide vector

Tags

Expression

Mutation

Promoter

PCs12

Available since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

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pRAM28

Plasmid

#234314

Purpose

pColE1 origin Cat-RNA U1376, Multiplexing ribozyme splicing reactions for the purpose of comparing host range of origins of replications

Depositor

James Chappell

Article

Kalvapalle et al Nat Biotechnol. 2025 Mar 18. doi: 10.103

Insert

U1367 Targeting RAM, Barcode 2

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

P. CymRC

Available since

March 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCasSA

Plasmid

#98211

Purpose

CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9

Depositor

Quanjiang Ji

Article

Chen et al J Am Chem Soc. 2017 Mar 15;139(10):3790-

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pdCas9-NED

Plasmid

#109358

Purpose

Mammalian vector for expressing effector domains fused to dCas9.

Depositor

Antal Kiss

Article

Goubert et al Methods Mol Biol. 2018;1767:395-415. doi

Type

Empty backbone

Use

CRISPR

Tags

dCas9, SV40 NLS, 3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pBS-L1PA1-CH-mneo

Plasmid

#51288

Purpose

contains the fully codon optimized ORF1 and ORF2 of the human L1RP

Depositor

Astrid Roy-Engel

Article

Wagstaff et al PLoS One. 2011 May 10;6(5):e19672. doi:

Insert

L1 (L1RE1 Human)

Use

Tags

mneoI indicator cassette containing an inverted n…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1a-4xGFP11-SEC61B-IRES-Hygro

Plasmid

#211468

Purpose

Lentivirus backbone expressing SEC61B protein tagged with 4 tandem GFP11 repeats at the N-terminal

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

4xGFP11-SEC61B

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1a-HA-ATAD3A-IRES-Hygro

Plasmid

#211469

Purpose

Lentivirus backbone expressing ATAD3A with an HA-tag at the N-terminal

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

ATAD3A (ATAD3A Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pBMN YFP-DHFR(DD)

Plasmid

#29326

Depositor

Thomas Wandless

Article

Iwamoto et al Chem Biol. 2010 Sep 24. 17(9):981-8.

Insert

YFP-DHFR(DD)

Use

Retroviral

Tags

HA and YFP

Expression

Mutation

Change Arg 12 to His (R12H),Asn 18 Thr (N18T), Va…

Promoter

Available since

April 1, 2011

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-NucGFP

Plasmid

#184706

Purpose

Lentiviral transfer plasmid for expression of NucGFP

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

NucGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pBMN DHFR(DD)-YFP

Plasmid

#29325

Depositor

Thomas Wandless

Article

Iwamoto et al Chem Biol. 2010 Sep 24. 17(9):981-8.

Insert

DHFR(DD)-YFP

Use

Retroviral

Tags

HA and YFP

Expression

Mutation

Change Arg 12 to Tyr (R12Y),Gly 67 to Ser (G67S) …

Promoter

Available since

July 1, 2011

Availability

Academic Institutions and Nonprofits only

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