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pJK220

Plasmid

#71123

Purpose

Expression of E. coli pantotheinate kinase (H6PanK, R175H mutant) in E. coli

Depositor

T. J. Kappock

Article

Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

Insert

pantothenate kinase

Tags

H6

Expression

Bacterial

Mutation

Arg175 is mutated to a His

Promoter

T7

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pET-21a(+)-T-protein

Plasmid

#12652

Depositor

SeWon Suh

Article

Lee et al J Biol Chem. 2004 Nov 26. 279(48):50514-

Insert

T-protein

Expression

Bacterial

Available Since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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pAT005

Plasmid

#247636

Purpose

to generate mRNA for membrane mStaygold(mBaoJin) for zebrafish

Depositor

Gokul Upadhyayula

Article

Tschanz et al MicroPubl Biol. 2025 Nov 25;2025:10.1791

Insert

2x Lyn Kinase membrane localization sequence

Use

Zebrafish mrna expression using sp6

Tags

mStayGold(BJ)

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pDQM049

Plasmid

#247635

Purpose

to generate mRNA for membrane mStayGold(E138D) for zebrafish

Depositor

Gokul Upadhyayula

Article

Tschanz et al MicroPubl Biol. 2025 Nov 25;2025:10.1791

Insert

2x Lyn Kinase membrane localization sequence

Use

Zebrafish mrna expression using sp6

Tags

mStayGold(E138D)

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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AGG1984 UbL40-Bs

Plasmid

#242439

Purpose

Ae aegypti UbL40 promoter expressing barstar

Depositor

Luke Alphey

Article

Nevard et al Commun Biol. 2025 Aug 4;8(1):1154. doi:

Insert

Barstar

Expression

Insect

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pSGEP-shLuciferase_3

Plasmid

#239617

Purpose

Control non-targeting shRNA

Depositor

Reza Kalhor

Article

Handler et al Cell Rep. 2025 Dec 15;44(12):116701. doi

Insert

shLuciferase_3

Use

Lentiviral

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pUdOm2.2

Plasmid

#210279

Purpose

Empty backbone with ampicillin resistance for high expression in mammalian cells

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pUdOm2.5

Plasmid

#210282

Purpose

Empty backbone with ampicillin resistance for low expression in mammalian cells

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pUdOm3.1

Plasmid

#210283

Purpose

Empty backbone with chloramphenicol resistance for high expression in mammalian cells

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pUdOm4.1

Plasmid

#210284

Purpose

Empty backbone with chloramphenicol resistance for high expression in mammalian cells

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pUdOm5.1

Plasmid

#210285

Purpose

Empty backbone with trimethoprim resistance for high expression in mammalian cells

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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cisRAJ31-cat

Plasmid

#69933

Purpose

Expresses cisRAJ31-cat, a cis-repressed mRNA regulated by RAJ31.

Depositor

Alfonso Jaramillo

Article

Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do

Insert

PllacO1-cisRAJ31-cat

Use

Synthetic Biology

Expression

Bacterial

Promoter

PlLacO1

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pJK227

Plasmid

#71126

Purpose

Expression of Acetobacter aceti 1023 citrate synthase (AaCS) in E. coli

Depositor

T. J. Kappock

Article

Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

Insert

citrate synthase

Tags

GyrA intein

Expression

Bacterial

Promoter

T7

Available Since

Jan. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pJK136

Plasmid

#71122

Purpose

Expression of Acetobacter aceti 1023 citrate synthase (AarA) in E. coli

Depositor

T. J. Kappock

Article

Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

Insert

citrate synthase

Expression

Bacterial

Promoter

T7

Available Since

Jan. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pBS-Int

Plasmid

#50000

Purpose

Expresses the integrase on a suicide plasmid

Depositor

Brian Robertson, Peter Sander

Article

Springer et al Int J Med Microbiol. 2001 Mar;290(8):669

Insert

Integrase from pMV361

Expression

Bacterial

Promoter

own

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pHAGE-RSV-tdTomato-2A-GCaMP6s

Plasmid

#80316

Purpose

Fluorescent reporter for calcium signaling

Depositor

Darrell Kotton

Article

Liberti WA et al Nat Neurosci. 2016 Oct 10. doi: 10.1038/

Inserts

tdTomato
GCaMP6s

Use

Lentiviral

Promoter

RSV

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pBigT

Plasmid

#21270

Purpose

Used for making transgenic mice. Contains a loxP-flanked cassette with a PGK-neo selectable marker and a tpA transcriptional stop sequence.

Depositor

Frank Costantini

Article

Srinivas et al BMC Dev Biol. 2001;1:4. doi: 10.1186/147

Type

Empty backbone

Use

Mouse Targeting

Available Since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

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pCpfSA

Plasmid

#187890

Purpose

CRISPR/Cpf1-based Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus

Depositor

Quanjiang Ji

Article

Wang et al ACS Synth Biol. 2022 Sep 16;11(9):3049-3

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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Halo-WIPI2B WT

Plasmid

#175025

Purpose

Expresses WIPI2B fused to Halo in mammalian cells

Depositor

Erika Holzbaur, Andrea Stavoe

Article

Stavoe et al Elife. 2019 Jul 16;8. pii: 44219. doi: 1

Insert

WIPI2B (WIPI2 Human)

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCN34e-yfp

Plasmid

#227641

Purpose

Negative control plasmid encoding yfp downstream of an attenuated ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

Depositor

Angelika Grundling

Article

Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

Insert

yfp

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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