We narrowed to 2,013 results for: ENDOG

1. pPW3755 : HDR-HsIRE1a-10xGS-HaloTag

   Plasmid

   #185677

   Purpose

   HDR template used for endogenous CRISPR editing of ERN1 gene, inserting a C-terminal HaloTag.

   Depositor

   Peter Walter

   Article

   Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

   Insert

   ERN1 homology arms (ERN1 Human, Synthetic)
   

   Use

   CRISPR

   Tags

   HaloTag

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS-RaichuEV-Rac1(Y40C)

   Plasmid

   #164903

   Purpose

   Expresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation Y40C in the Rac1 domain. More useful than T17N mutation due to less harmful to endogenous signaling.

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   RaichuEV-Rac1 (RAC1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Y40C mutation in the Rac1 domain whcih make the s…

   Promoter

   Available since

   Aug. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Flag_HsIRE1a_pBabePuro

   Plasmid

   #54337

   Purpose

   N-terminally FLAG tagged human IRE1a in retroviral pBabe puro vector

   Depositor

   David Ron

   Article

   Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11

   Insert

   IRE1a (ERN1 Human)
   

   Use

   Retroviral

   Tags

   FLAG and preprotrypsin signal sequence

   Expression

   Mammalian

   Mutation

   The endogenous IRE1a signal sequence (aa1-18) was…

   Promoter

   Available since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDisplay-DNER

   Plasmid

   #51053

   Purpose

   expression of HA-DNER in mammalian cells

   Depositor

   Mineko Kengaku

   Article

   Eiraku et al J Biol Chem. 2002 Jul 12;277(28):25400-7

   Insert

   DNER (Dner Mouse)
   

   Use

   Tags

   HA and signal peptide

   Expression

   Mammalian

   Mutation

   without endogenous signal peptide (1-221bp)

   Promoter

   CMV

   Available since

   Feb. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pBactin-mOrange2-NEDD1-gTBD

   Plasmid

   #196858

   Purpose

   Expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange. Used to displace endogenous gamma-TuRC from the centrosome.

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   mOrange2-N-gTBD (NEDD1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBactin-EGFP-NEDD1-gTBD

   Plasmid

   #196857

   Purpose

   Expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to enhanced (E) GFP. Used to displace endogenous gamma-TuRC from the centrosome.

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   EGFP-N-gTBD (NEDD1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. PF3D7_1136200-bio

   Plasmid

   #47730

   Purpose

   Expresses enzymatically monobiotinylated full-length PF3D7_1136200 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised PF3D7_1136200
   

   Use

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin replaced…

   Promoter

   CMV

   Available since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHS0535 CRISPR-associated IscB (Chesapeake Bay) in pBR322 with Fn spacer

   Plasmid

   #176589

   Purpose

   Endogenous locus of CRISPR-associated IscB (Chesapeake Bay) with Fn spacer

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Insert

   CRISPR-associate IscB (Chesapeake Bay) locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Truncated CRISPR array to two direct repeats, rep…

   Promoter

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBrain-GFP-TACC3KDP(L566,567A)-shTACC3

   Plasmid

   #69117

   Purpose

   Dual-promoter plasmid to express knockdown-proof human TACC3 (L566A and L567A substitutions) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.

   Depositor

   Stephen Royle

   Article

   Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

   Inserts

   Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
   shTACC3
   

   Use

   RNAi

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   LL (566-567aa) mutated to AA. Silent mutations i…

   Promoter

   CMV

   Available since

   Feb. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. human SLC12A5-V5-HA-2A-Luciferase repair template

    Plasmid

    #158576

    Purpose

    For targeted insertion of V5-HA-2A-Luciferase reporter before the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.

    Depositor

    Rudolf Jaenisch

    Article

    Tang et al Sci Transl Med. 2019 Jul 31;11(503). pii

    Insert

    human SLC12A5 5' homology arm-V5-HA-2A-Luciferase-3' homology arm (SLC12A5 Human)
    

    Use

    Tags

    V5 and HA on the endogenous KCC2 protein product

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFUGW-shRIIα-RIIαWT-IRES-EGFP

    Plasmid

    #183456

    Purpose

    Replacement of endogenous rat PKA-RIIα subunits with wild-type RIIα

    Depositor

    Matthew Gold

    Article

    Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

    Insert

    sgRNA and Prkar2a (Prkar2a Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    shRNA-resistant mutations: C135>G, G138>A, …

    Promoter

    shRNA: H1 / gene: ubiquitin

    Available since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBrain-GFP-TACC3KDP∆678-681-shTACC3

    Plasmid

    #69115

    Purpose

    Dual-promoter plasmid to express knockdown-proof human TACC3 (amino acids 678-681 deleted) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.

    Depositor

    Stephen Royle

    Article

    Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

    Inserts

    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    shTACC3
    

    Use

    RNAi

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deletion of 678-681aa region. Silent mutations i…

    Promoter

    CMV

    Available since

    Oct. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR2b-ITGB1(WT)

    Plasmid

    #215450

    Purpose

    Gateway entry vector with integrin beta1 wild-type (WT)

    Depositor

    Johanna Ivaska

    Article

    Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

    Insert

    ITGB1 (ITGB1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Silent mutations added to disrupt shRNA binding a…

    Promoter

    none

    Available since

    May 29, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-lk-HiBiT

    Plasmid

    #233064

    Purpose

    Expresses mouse ADGRL3 (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide. Also includes a C-terminal HiBiT tag.

    Depositor

    Jonathan Javitch

    Article

    Perry-Hauser et al J Biol Chem. 2025 Jan 9:108174. doi: 10.

    Insert

    ADGRL3 (Adgrl3 Mouse)
    

    Use

    Tags

    ALFA and HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-T923G

    Plasmid

    #233063

    Purpose

    Expresses cleavage-deficient mouse ADGRL3-T923G (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide.

    Depositor

    Jonathan Javitch

    Article

    Perry-Hauser et al J Biol Chem. 2025 Jan 9:108174. doi: 10.

    Insert

    ADGRL3 (Adgrl3 Mouse)
    

    Use

    Tags

    ALFA and HiBiT

    Expression

    Mammalian

    Mutation

    Threonine 923 to Glycine

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHES7-NLuc-2A-tdTomato

    Plasmid

    #204348

    Purpose

    Donor vector to tag endogenous pig HES7 locus

    Depositor

    Li-Fang Chu

    Article

    Conrad et al Stem Cell Reports. 2023 Dec 12;18(12):23

    Insert

    NLuc-2A-tdTomato
    

    Use

    Tags

    NanoLuc and tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-lox-inv[Talpha1-iCre-pA]-lox-mOrange2-NEDD1-gTBD

    Plasmid

    #196878

    Purpose

    Neuron-specific expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange. Used to displace endogenous gamma-TuRC from the centrosome.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    mOrange2-N-gTBD
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Quimeric CAG

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-lox-inv[Talpha1-Dre-pA]-lox-mOrange2-Cdk5rap2-CTD

    Plasmid

    #196881

    Purpose

    Neuron-specific expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to mOrange2. Used to displace endogenous Cdk5rap2 from centrosomes.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    mOrange2-Cdk5rap2-CTD
    

    Use

    Cre/Lox; Dre/rox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Quimeric CAG

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. fru-T2A-CsChrimson-tdTomato HDR plasmid

    Plasmid

    #141100

    Purpose

    Plasmid for CRISPR-generated knock-in line that expresses optogenetic activator CsChrimson-tdTomato by knocking-in fragment into the coding sequence of the endogenous Aedes aegypti fru gene

    Depositor

    Leslie Vosshall

    Article

    Basrur et al Elife. 2020 Dec 7;9. pii: 63982. doi: 10

    Insert

    fru-left-HDR-arm; T2A-CsChrimson-tdTomato-SV40; 3xP3-EYFP-SV40; fru-right-HDR-arm
    

    Use

    Donor template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFUGW-shRIIα-RIIαS97A-IRES-EGFP

    Plasmid

    #183457

    Purpose

    Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97A variant

    Depositor

    Matthew Gold

    Article

    Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

    Insert

    sgRNA and Prkar2a (Prkar2a Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    shRNA-resistant mutations: C135>G, G138>A, …

    Promoter

    shRNA: H1 / gene: ubiquitin

    Available since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge