We narrowed to 9,863 results for: 158

1. pJK524

   Plasmid

   #71580

   Purpose

   Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Asn347>Ala mutant (AarCH6-N347A)

   Depositor

   T. J. Kappock

   Article

   Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

   Insert

   succinyl-CoA:acetate CoA-transferase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   changes asparagine-347 to alanine

   Promoter

   T7

   Available since

   April 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. Sema4f.a-Fc-His

   Plasmid

   #72158

   Purpose

   Expresses the extracellular region of the Sema4F, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Sema4f.a (Sema4f Mouse)
   

   Use

   Tags

   Fc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJK564

   Plasmid

   #71581

   Purpose

   Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Ser71>Ala mutant (AarCH6-S71A)

   Depositor

   T. J. Kappock

   Article

   Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

   Insert

   succinyl-CoA:acetate CoA-transferase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   changes serine-71 to alanine

   Promoter

   T7

   Available since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMuLE ENTR CMV iRFP R4-R3

   Plasmid

   #62158

   Purpose

   MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and iRFP module. Compatible with MultiSite Gateway cloning

   Depositor

   Ian Frew

   Article

   Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

   Insert

   iRFP
   

   Use

   Mule gateway entry vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLEX_305-N-dTAG

   Plasmid

   #91797

   Purpose

   Lentiviral/gateway cloning vector for N-terminally tagging proteins of interest with the dTAG system

   Depositor

   Behnam Nabet, James Bradner

   Article

   Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   FKBP F36V

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK

   Available since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. phCMV-GALV-MTR

   Plasmid

   #163612

   Purpose

   Retro and Lentiviral transduction of primary human germinal center B cells

   Depositor

   Daniel Hodson

   Article

   Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

   Insert

   GaLV MTR envelope
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330A-nBRD4/PITCh

   Plasmid

   #91794

   Purpose

   PITCh sgRNA, N-terminal BRD4 sgRNA, and Cas9 expressing plasmid for use with the dTAG knock-in system and BRD4

   Depositor

   Behnam Nabet, James Bradner

   Article

   Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   chicken β-actin promoter

   Available since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. EXT1-pLJC2-3XFLAG

   Plasmid

   #215818

   Purpose

   Ectopic expression of EXT-1 in mammalian cells

   Depositor

   Yoav Shaul

   Article

   Solaimuthu et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

   Insert

   Exostosin glycosyltransferase 1 (EXT1 Human)
   

   Use

   Lentiviral

   Tags

   3X Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLX307-CRBN-3F-BirA

   Plasmid

   #220146

   Purpose

   Expresses CRBN-BirA for CRBN substrate identification by E-STUB

   Depositor

   William Sellers

   Article

   Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

   Insert

   Cereblon (CRBN Human)
   

   Use

   Lentiviral

   Tags

   3F-BirA

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. VRK1-dTag-puro

    Plasmid

    #199651

    Purpose

    Expresses VRK1 C-term. Tagged with dTAG

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    VRK1 (VRK1 Human)
    

    Use

    Lentiviral

    Tags

    dTAG

    Expression

    Mutation

    Promoter

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEFIRES-P-ACSL3-mCherry

    Plasmid

    #87158

    Purpose

    Fluorescent protein targeted to lipid droplets (LDs) from the endoplasmic reticulum (ER)

    Depositor

    Elina Ikonen

    Article

    Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

    Insert

    ACSL3 (ACSL3 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1-alpha

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. hSynapsin-PE2-Cterminal-lenti

    Plasmid

    #135956

    Purpose

    Neuronal expression of C-terminal intein-split prime editor v2 (PE2). This plasmid is used by PE2, PE3, and PE3b

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    Prime editor v2 (PE2) C-terminal
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    MMLV RT(D200N, T306K, W313F, T330P, L603W)

    Promoter

    hSynapsin

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAJ075_hsCRBN

    Plasmid

    #124214

    Purpose

    Insect cell expression vector for His6 tagged hsCRBN

    Depositor

    Eric Fischer

    Article

    Nowak et al Nat Chem Biol. 2018 Jul;14(7):706-714. d

    Insert

    Cereblon (CRBN Human)
    

    Use

    Tags

    His6-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDF0114 pU6-Eco31i-Eco31i-DisCas7-11 mature DR guide scaffold with golden gate site

    Plasmid

    #172508

    Purpose

    Encodes the mature DisCas7-11 DR along with a golden gate site for spacer cloning

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    pU6-Eco31i-Eco31i-DisCas7-11 mature DR guide scaffold with golden gate site
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLX317-VRK1-K179E

    Plasmid

    #199644

    Purpose

    Expresses kinase-inactive VRK1 (K179E)

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    VRK1 (VRK1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    K179E

    Promoter

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LentiCas9-sgVRK1 #4-Blast

    Plasmid

    #199647

    Purpose

    Expresses Cas9 and sgRNA guide targeting VRK1

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    N/A (VRK1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lenti-TargetBC-5xTAPE-1-pegRNA-InsertBC

    Plasmid

    #183790

    Purpose

    A single construct out of the pool of plasmid for lineage recording experiment using DNA Ticker Tape.

    Depositor

    Jay Shendure

    Article

    Choi et al Nature. 2022 Jul 6. pii: 10.1038/s41586-

    Inserts

    P2A-eGFP-TargetBC-5xTAPE-1
    pegRNA-InsertBC
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    G>A in the gRNA scaffold- please see depositor…

    Promoter

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28C-mCherry-MED1LCD

    Plasmid

    #204404

    Purpose

    E. coli expression of MED1 low-complexity domain (947-1581) with an N-terminal mCherry tag

    Depositor

    Samie Jaffrey

    Article

    Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

    Insert

    MED1(947-1581) (MED1 Human)
    

    Use

    Tags

    6x-His; mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1 HA-FAM208A

    Plasmid

    #115845

    Purpose

    Encodes codon optimized HA tagged FAM208A

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    HA tagged FAM208A (C3orf63 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. IMPT-10314:GABBR2

    Plasmid

    #157851

    Purpose

    HA-3xFLAG-VFT-TMD-CC in pFastBac1 for Sf9 expression

    Depositor

    Vadim Cherezov, Raymond Stevens

    Article

    Shaye et al Nature. 2020 Jun 17. pii: 10.1038/s41586

    Insert

    GABBR2 (GABBR2 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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