We narrowed to 2,218 results for: dcas9

1. pBT44c

   Plasmid

   #122600

   Purpose

   BE PACE selection, express C-intein-Cas9-ugi fusion

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   intein-dCas9-ugi fusion
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSB-TRE-CRISPRoff-EF1A-TetOn

   Plasmid

   #203355

   Purpose

   Drug-inducible expression of CRISPRoff components needed for DNA methylation and gene repression with Sleeping Beauty backbone

   Depositor

   Jonathan Brunger

   Article

   Gil et al Bio Protoc. 2024 Jan 5;14(1):e4913. doi:

   Insert

   CRISPRoff-TetOn
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISPRi_Mxi1_yl_NHEJ

   Plasmid

   #91249

   Purpose

   CRISPRi vector for Yarrowia lipolytica, repressing KU70 and KU80 for enhanced HR

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.

   Inserts

   Codon optimized dCas9-Mxi1
   KU70 sgRNA expression cassette
   KU80a sgRNA expression cassette
   KU80b sgRNA expression cassette
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SCR1'-tRNA and UAS1B8-TEF(136)

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS156

   Plasmid

   #182707

   Purpose

   CRISPRa plasmid for Candida albicans (dCas9-VPR and sgRNA cloning site)

   Depositor

   Rebecca Shapiro

   Article

   Gervais et al G3 (Bethesda). 2022 Dec 1:jkac301. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-TetON-dual-SunTag-Hygro

   Plasmid

   #121119

   Purpose

   Endogenous gene activation with the SunTag CRISPR activation system

   Depositor

   Pablo Navarro

   Article

   Heurtier et al Nat Commun. 2019 Mar 7;10(1):1109. doi:

   Insert

   dCas9-GCN4x10_scFv-VP64
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tet-ON

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGCP123-EbpA_g1

   Plasmid

   #153517

   Purpose

   sgRNA for ebpA gene (NT) under nisin-inducible nisA promoter, barcoded

   Depositor

   Kimberly Kline

   Article

   Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

   Insert

   pnisA-sgRNA(EbpA_g1)-dCas9 scaffold
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-dSa-VP64-p65(100-261)-RTA(125-190)

   Plasmid

   #99679

   Purpose

   Expresses dSa Cas9 fused to optimized combination of truncated activation domains

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9
   

   Use

   AAV

   Tags

   VP64-p65(101-261)-RTA(125-190)

   Expression

   Mutation

   dead Cas9

   Promoter

   Available Since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. dABE

   Plasmid

   #124447

   Purpose

   Expresses dead Cas9 (dCas9)-ABE7.10

   Depositor

   George Church

   Article

   Smith et al Nucleic Acids Res. 2020 May 21;48(9):518

   Insert

   dABE7.10
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   Available Since

   April 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. RFN_EGFP_81

   Plasmid

   #60073

   Purpose

   Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

   Depositor

   Keith Joung

   Article

   Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

   Insert

   pair of multiplex gRNAs targeting EGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Oct. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pLG1-puro-sgATL3-2

    Plasmid

    #109010

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    ATL3-sgRNA #2 (ATL3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 promoter

    Available Since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRP_8xgRNA-FLuc

    Plasmid

    #135937

    Purpose

    Firefly luciferase reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activation.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    8 copies of gRNA binding site (5'-AAAGGTCGAGAAACTGCAAA-3')
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    minCMV

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. RFN_EGFP_47

    Plasmid

    #60071

    Purpose

    Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    pair of multiplex gRNAs targeting EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Oct. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pLG1-puro-sgATL3-5

    Plasmid

    #109011

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    ATL3-sgRNA #5 (ATL3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 promoter

    Available Since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. RFN_EGFP_84

    Plasmid

    #60076

    Purpose

    Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    pair of multiplex gRNAs targeting EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Oct. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

15. RFN_EGFP_83

    Plasmid

    #60075

    Purpose

    Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    pair of multiplex gRNAs targeting EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Oct. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

16. RFN_EGFP_82

    Plasmid

    #60074

    Purpose

    Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    pair of multiplex gRNAs targeting EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Oct. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

17. RFN_EGFP_80

    Plasmid

    #60072

    Purpose

    Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    pair of multiplex gRNAs targeting EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Oct. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

18. lentiSAMv2

    Plasmid

    #75112

    Purpose

    lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and EF1A

    Available Since

    May 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEG302_22aa_SunTag_MQ1(Q147L)_g4+g10+g18

    Plasmid

    #172318

    Purpose

    CRISPR dCas9 SunTag system to target a variant of bacterial DNA methyltransferase MQ1 to install CG specific methylation to the FWA locus with three guide RNAs

    Depositor

    Steven Jacobsen

    Article

    Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    g18_U6_g10_U6_g4_U6_NOS_MQ1(Q147L)_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCRISPRa_all-in-one

    Plasmid

    #183695

    Purpose

    Expresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selective

    Depositor

    Edward Avezov

    Article

    Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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