We narrowed to 7,174 results for: ef1a

1. pDIO-DSE-mCherry-PSE-MCS

   Plasmid

   #129669

   Purpose

   Backbone that can be used to generate AAV virus where, upon cre-specific recombination, mCherry and any shRNA can be simultaneously expressed

   Depositor

   Beatriz Rico

   Article

   Favuzzi et al Science. 2019 Jan 25;363(6425):413-417.

   Type

   Empty backbone

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-EWB-DIO-cyan pre-eGRASP(p30)

   Plasmid

   #111583

   Purpose

   An AAV vector that expresses double floxed cyan pre-eGRASP(p30) under the Ef1a promoter.

   Depositor

   Bong-Kiun Kaang

   Article

   Choi et al Science. 2018 Apr 27;360(6387):430-435.

   Insert

   cyan pre-eGRASP(p30)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   June 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEF-GFP

   Plasmid

   #11154

   Purpose

   Mammalian expression vector for expression of GFP (EF1a promoter)

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

   Has Service

   Cloning Grade DNA

   Insert

   EF1 alpha promoter (EEF1A1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-EWB-DIO-cyan pre-eGRASP(p32)

   Plasmid

   #111589

   Purpose

   An AAV vector that expresses double floxed cyan pre-eGRASP(p32) under the Ef1a promoter.

   Depositor

   Bong-Kiun Kaang

   Article

   Choi et al Science. 2018 Apr 27;360(6387):430-435.

   Insert

   cyan pre-eGRASP(p32)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   June 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. HIVGKO

   Plasmid

   #112234

   Purpose

   HIVGKO contains a codon-switched eGFP under the control of the HIV-1 specific promoter, and mKO2 under the control of the constitutive promoter EF1a

   Depositor

   Eric Verdin

   Article

   Battivelli et al Elife. 2018 May 1;7. pii: 34655. doi: 10

   Insert

   csGFP-EF1a-mKO2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-DIO-EF1α-mKate2-biPAC

   Plasmid

   #169127

   Purpose

   AAV plasmid containing a mKate2 and a light-gated adenylate cyclase biPAC

   Depositor

   Georg Nagel

   Article

   Zhang et al Nature. 2021 Sep;597(7875):245-249. doi:

   Insert

   mKate2-biPAC
   

   Use

   AAV and Cre/Lox

   Tags

   mKate2

   Expression

   Mammalian

   Mutation

   codon optimized to mouse

   Promoter

   EF1a

   Available since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-Ftractin-mRuby3-p2A-mTurquoise-MLC-IRES-Blast

   Plasmid

   #85146

   Purpose

   lentiviral expression of a stoichiometric F-actin and myosin light chain reporter

   Depositor

   Tobias Meyer

   Article

   Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

   Insert

   Ftractin-mRuby3-P2A-mTurquoise-MLC
   

   Use

   Lentiviral

   Tags

   mRuby3, mTurquoise

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5FRT-EF-Pdgfralpha-EGFPN D842V

   Plasmid

   #66789

   Purpose

   Expression of constitutively active murine PDGFRalpha D842V tagged with GFP under the control of EF1a promoter

   Depositor

   Lotte Pedersen

   Article

   Nielsen et al J Cell Sci. 2015 Oct 1;128(19):3543-9. d

   Insert

   PDGFRalpha (Pdgfra Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   D842V (constitutively active)

   Promoter

   EF1a

   Available since

   July 14, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pLV-RhoB Sensor (PKN-L59Q)-IRES-Blast

   Plasmid

   #222634

   Purpose

   Rho reporter, sensor dead

   Depositor

   Arnold Hayer

   Article

   Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

   Insert

   RhoB Sensor (PKN-L59Q)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   PKN L59Q

   Promoter

   EF1a

   Available since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Empty Target Control

    Plasmid

    #127667

    Purpose

    Encodes for the CRY2-HA and mCherry proteins expressed from the EF1a promoter

    Depositor

    Jennifer Phillips-Cremins

    Article

    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

    Insert

    CRY2-HA-2A-mCherry
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. lenti dCAS-VP64_Blast

    Plasmid

    #61425

    Purpose

    3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE)

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Lentiviral Prep

    Insert

    dCAS9(D10A, N863A)-VP64_2A_Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9

    Promoter

    EF1A

    Available since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. lenti sgRNA(MS2)_zeo backbone

    Plasmid

    #61427

    Purpose

    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and EF1A

    Available since

    Dec. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. phDLX1-N174

    Plasmid

    #60859

    Purpose

    2nd generation lentiviral transfer plasmid. Expresses DLX1 under the EF1a promoter

    Depositor

    Andrew Yoo

    Article

    Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

    Insert

    DLX1 (DLX1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. lenti sgRNA(MS2)_puro optimized backbone

    Plasmid

    #73797

    Purpose

    optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and EF1A

    Available since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. dCas9-hHDAC3

    Plasmid

    #98591

    Purpose

    3rd generation lenti vector encoding dCas9-hHDAC3 (EF1a-NLS-dCas9(N863)-hHDAC3). Expresses dCas9 fused to human HDAC3.

    Depositor

    Zhaolan Zhou

    Article

    Kwon et al Nat Commun. 2017 May 12;8:15315. doi: 10

    Insert

    dCAS9-HDAC3 (HDAC3 Synthetic, Human, S. pyogenes)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9

    Promoter

    EF1A

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-EWB-DIO-myriRFP670V5-P2A-post-eGRASP

    Plasmid

    #111585

    Purpose

    An AAV vector that expresses double floxed myristoylated iRFP670 with V5 tag and post-eGRASP linked by self-cleaving P2A peptide under the Ef1a promoter.

    Depositor

    Bong-Kiun Kaang

    Article

    Choi et al Science. 2018 Apr 27;360(6387):430-435.

    Insert

    myriRFP670V5-P2A-post-eGRASP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    June 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. lenti sgRNA(MS2)_puro backbone

    Plasmid

    #73795

    Purpose

    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and EF1A

    Available since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEF1α:flex-mcherry

    Plasmid

    #222618

    Purpose

    Cre-dependent expression of the red fluorescent protein mCherry under control of the EF1a promoter

    Depositor

    Franck Polleux

    Article

    Blockus et al Cell Rep. 2021 Oct 19;37(3):109828. doi:

    Insert

    Cre-dependent mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-EWB-DIO-myrTagRFP-T-P2A-post-eGRASP

    Plasmid

    #111581

    Purpose

    An AAV vector that expresses double floxed myristoylated TagRFP-T and post-eGRASP linked by self-cleaving P2A peptide under the Ef1a promoter.

    Depositor

    Bong-Kiun Kaang

    Article

    Choi et al Science. 2018 Apr 27;360(6387):430-435.

    Insert

    myrTagRFP-T-P2A-post-eGRASP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    June 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-pCaMKII-EBFP-tTAn

    Plasmid

    #172124

    Purpose

    Expression of EBFP and tTA-InteinN in CaMKII positive cells

    Depositor

    Chun Xu

    Article

    Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

    Insert

    pCaMKII, EBFP, tTAn-InteinN
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge