Addgene

We narrowed to 2,757 results for: fas

Showing: 1881 - 1900 of 2757 results

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pLKO.1-Tet-Puro-shIAPEz_1

Plasmid

#185016

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_IAPEz

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-nLight3.1

Plasmid

#111061

Purpose

Expresses nLight3.1 in mammalian cells

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Insert

nLight3.1

Use

Tags

Flag tag

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-Avi-His6_IRES-mTurquoise2

Plasmid

#113890

Purpose

Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic mTurquoise2

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Avi-His6

Expression

Mutation

Promoter

CMV-MIE-TetO2

Available since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-FlicR1

Plasmid

#74143

Purpose

Express FlicR1 preferentially in neurons

Depositor

Robert Campbell

Article

Abdelfattah et al J Neurosci. 2016 Feb 24;36(8):2458-72. d

Insert

FlicR1

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-LGC-53

Plasmid

#86399

Purpose

Mammalian expression of codon-optimized dopamine receptor LGC-53

Depositor

Steven Mennerick

Article

Kress et al J Neurosci. 2014 Aug 27;34(35):11792-802

Insert

LGC-53 (lgc-53 Nematode, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Codon optimized for mammalian expression; Signal …

Promoter

CMV

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pUC19.TRIM28.FKBP.knock-in.mRuby2

Plasmid

#185008

Purpose

For knocking in FKBP F36V P2A mRuby2

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

FKBP F36V P2A mRuby2

Use

Cloning

Tags

Expression

Mutation

Promoter

Available since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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CVS-N2c-tdTomato-iCre

Plasmid

#172374

Purpose

Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

tdTomato + iCre

Use

Neurotropic virus

Tags

Expression

Mutation

Promoter

Available since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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Venus-7aa-ActA-pEGFP-C1

Plasmid

#177405

Purpose

To express Venus fused to mitochondria outer membrane targeting sequence "ActA". Venus faces the cytosol.

Depositor

David Andrews

Article

Osterlund et al Protocol Exchange (2022)

Insert

ActA tail anchor (actA Listeria monocytogenes)

Use

Tags

Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-Avi-His6_IRES-mRuby2

Plasmid

#113889

Purpose

Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic mRuby2

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Avi-His6

Expression

Mutation

Promoter

CMV-MIE-TetO2

Available since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-dLight1.5

Plasmid

#111058

Purpose

Expresses dLight1.5 in mammalian cells

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Insert

dLight1.5

Use

Tags

Flag tag

Expression

Mammalian

Mutation

Promoter

Available since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-Flex-PAK1-AID-ires-EGFP

Plasmid

#83489

Purpose

cre-dependent PAK1-AID under EF1a promoter

Depositor

Dong Kong

Article

Kong et al Neuron. 2016 Jun 16. pii: S0896-6273(16)

Insert

PAK1-AID-ires-EGFP

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-DIO-mDLX-TVA-2A-N2cG

Plasmid

#172365

Purpose

Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from cre-expressing inhibitory neurons

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

TVA + B19-N2cG

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

mDLX

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a His6Flag2AUF1(p37)

Plasmid

#135939

Purpose

Expresses AUF1p37 in E. coli

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)

Use

Tags

Flag2 and His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a His6Flag2AUF1(p45)

Plasmid

#135942

Purpose

Expresses AUF1p45 in E. coli

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)

Use

Tags

Flag2 and His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-Flex-CA-AMPK-H150R-T2A-mCherry

Plasmid

#83490

Purpose

cre-dependent CA-AMPK under EF1a promoter

Depositor

Dong Kong

Article

Kong et al Neuron. 2016 Jun 16. pii: S0896-6273(16)

Insert

CA-AMPK-2A-mCherry

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

H150R

Promoter

Available since

Oct. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-DIO-CaMKII-TVA-P2A-N2cG

Plasmid

#172362

Purpose

Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from cre-expressing excitatory cortical neurons

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

TVA + B19-N2cG

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

CaMKIIa

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pRiboI-APEX2m

Plasmid

#176843

Purpose

Expression of APEX2 from a codon-optimized gene (single-copy integrating plasmid)

Depositor

Jessica Seeliger

Article

Ahamed et al Methods Enzymol. 2022;664:267-289. doi:

Insert

pRibo-APEX2m

Use

Integrating

Tags

Expression

Bacterial

Mutation

Promoter

pRibo, theophilline riboswitch inducible hsp60 pr…

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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CVS-N2c-nl.EGFP-jRGECO1a

Plasmid

#172386

Purpose

Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

nuclear-localized EGFP + jRGECO1a

Use

Neurotropic virus

Tags

Expression

Mutation

Promoter

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-dLight1.3a

Plasmid

#111055

Purpose

Expresses dLight1.3a in mammalian cells

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Insert

dLight1.3a

Use

Tags

Flag tag

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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mTagRFP-T-EB3-7

Plasmid

#58012

Purpose

Localization: MT End Binding Protein, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Robert et al FASEB J. 2014 Mar 20.

Insert

EB3 (MAPRE3 Human)

Use

Tags

mTagRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits