We narrowed to 13,565 results for: cas9 genes

1. pMuLE ENTR SV40-hCas9 L3-L2

   Plasmid

   #62133

   Purpose

   MuLE (Multiple Lentiviral Expression) Entry vector containing SV40 promoter and human codon optimized Cas9 module. Compatible with MultiSite Gateway cloning

   Depositor

   Ian Frew

   Article

   Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

   Insert

   human codon optimized Cas9
   

   Use

   CRISPR; Mule gateway entry vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. A3H HapII-Cas9n-UGI

   Plasmid

   #119141

   Purpose

   Base editor made from APOBEC3H Haplotype II

   Depositor

   Reuben Harris

   Article

   Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

   Insert

   APOBEC3H Haplotype II-Cas9 nickase-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   None

   Promoter

   CMV

   Available since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA pDEST40 Cas9 GW STOP

   Plasmid

   #183196

   Purpose

   Destination vector

   Depositor

   Emma Haapaniemi

   Article

   Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT7-SpCas9_sgRNA-site2 (RTW448)

   Plasmid

   #160137

   Purpose

   T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #2

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

   Insert

   SpCas9 sgRNA with spacer #2 (spacer=GTCGCCCTCGAACTTCACCT)
   

   Use

   In vitro transcription of sgrna from t7 promoter

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. PX544 SpCas9(del1098-1368)

   Plasmid

   #58889

   Purpose

   Truncated form of SpCas9 without C' domain

   Depositor

   Feng Zhang

   Article

   Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   del amino acids 1098-1368

   Promoter

   Available since

   Sept. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-FLEX-SaCas9-U6-sgGabrg1

   Plasmid

   #124856

   Purpose

   Mutagenesis of Gabrg1

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Gabrg1 (Gabrg1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGB2alpha2 35s:hCas9:tNos (GB0639)

   Plasmid

   #68222

   Purpose

   Transcriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   hCas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   35S

   Available since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. px335-sgRNA-EF(Cas9wt)-Rif1

   Plasmid

   #122306

   Purpose

   Expresses sgRNA targeting mouse Rif1 and Cas9 in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   sgRNA for mouse Rif1 (Rif1 Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAWp63-clone32 (Opti-SpCas9)

   Plasmid

   #131736

   Purpose

   Expresses Opti-SpCas9 in mammalian cells

   Depositor

   Alan Siu Lun Wong

   Article

   Choi et al Nat Methods. 2019 Aug;16(8):722-730. doi

   Insert

   Opti-SpCas9
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mutation

   R661A+K1003H

   Promoter

   EFS

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-dHeFSpCas9-VP64-6xHis

    Plasmid

    #92119

    Purpose

    Expression of dead/inactive increased fidelity HeFSpCas9-VP64-6xHis in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive HeFSpCas9-NLS-3xFLAG-VP64
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, NLS, and VP64

    Expression

    Bacterial

    Mutation

    D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…

    Promoter

    T7

    Available since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV_RBMX-eA3A-UdgX-NG-nCas9

    Plasmid

    #163551

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    RBMX-eA3A-UdgX-NG-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

    Promoter

    Available since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-FLEXfrt-SaCas9-U6-sgGrin2b

    Plasmid

    #124864

    Purpose

    Mutagenesis of Grin2b

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Grin2b (Grin2b Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-FLEX-SaCas9-U6-sgKcnab2

    Plasmid

    #192795

    Purpose

    Encodes SaCas9 and an sgRNA targeting mouse Kcnab2

    Depositor

    Marta Soden

    Article

    Yee et al J Neurophysiol. 2022 Jul 1;128(1):62-72.

    Insert

    sgKcnab2
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. PX390 Flavobacterium columnare Cas9

    Plasmid

    #68327

    Purpose

    Flavobacterium columnare Cas9

    Depositor

    Feng Zhang

    Insert

    FcCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. lenti-Cas9-VQR-GFP_activity_reporter

    Plasmid

    #87156

    Purpose

    This lentiviral vector can be used to assay activity of SpCas9-VQR (NGA PAM restricted).

    Depositor

    Daniel Bauer

    Article

    Canver et al Nat Genet. 2017 Feb 20. doi: 10.1038/ng.

    Insert

    GFP and sgRNA targeting GFP (NGA restricted)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC CBA-SpCas9N863Anickase.EF1a-BFP.sgLMNApair1

    Plasmid

    #98976

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9N863A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 44bp 3' overhangs. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNAs pair 1 and Cas9(N863A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. px335-sgRNA-EF(Cas9n)-Eif4a1-R

    Plasmid

    #122345

    Purpose

    Expresses sgRNA targeting mouse Eif4a1 and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Eif4a1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-FLAG-eSpCas9-plus

    Plasmid

    #126774

    Purpose

    Expression of increased fidelity eSpCas9-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    eSpCas9-plus
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, MBP, and NLS

    Expression

    Bacterial

    Mutation

    K848A, R1060A; amino acids 1005-1013 replaced wit…

    Promoter

    T7

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. p-dCas9-HDAC1-H141A-Hygro

    Plasmid

    #104417

    Purpose

    transient expression of dCas9-dHDAC1 fusion protein

    Depositor

    Stephan Beck

    Insert

    dHDAC1 (HDAC1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    H141A

    Promoter

    CMV promoter

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pME-nCas9n-P2A-mKate2-pA

    Plasmid

    #109547

    Purpose

    Multisite gateway vector for expression of nCas9n with bicistronic expression of mKate2

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    nCas9n and mKate2
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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