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pRS413-MET25pr-CFAPoptim

Plasmid

#221105

Purpose

FAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS415-CUP1pr-NFAPoptim

Plasmid

#221107

Purpose

FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS415-CUP1pr-CFAPoptim

Plasmid

#221108

Purpose

FAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Type

Empty backbone

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pGFP-CUG-IRES-mCherry

Plasmid

#222110

Purpose

Start codon reporter with mutated start codon

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

GFP-IRES-mCherry

Use

Lentiviral

Mutation

CUG start codon

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-KaNLuc

Plasmid

#208855

Purpose

Expresses FLAG epitope-tagged KaNLuc "Red" BRET reporter in mammalian cells.

Depositor

Antonio Amelio

Article

Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

Insert

KaNLuc LumiFluor

Use

Lentiviral

Tags

1xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-Lumiscarlet

Plasmid

#208854

Purpose

Expresses FLAG epitope-tagged Lumiscarlet "Red" BRET reporter in mammalian cells.

Depositor

Antonio Amelio

Article

Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

Insert

Lumiscarlet

Use

Lentiviral

Tags

1xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-dKeNLuc

Plasmid

#208856

Purpose

Expresses FLAG epitope-tagged dKeNLuc "Red" BRET reporter in mammalian cells.

Depositor

Antonio Amelio

Article

Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

Insert

dKeNLuc LumiFluor

Use

Lentiviral

Tags

1xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-CeNLuc

Plasmid

#208838

Purpose

Expresses FLAG epitope-tagged CeNLuc "Blue" BRET reporter in mammalian cells.

Depositor

Antonio Amelio

Article

Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

Insert

CeNLuc LumiFluor

Use

Lentiviral

Tags

1xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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Halo-MDC1 BRCT domain deletion

Plasmid

#207110

Purpose

pHTN HaloTag CMV-neo (Promega; #G7721) based vector to express HaloTag-MDC1 with a deletion of the BRCT domain in human cells.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

HaloTag-MDC1 BRCT domain deletion

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_8xTEAD_luc2

Plasmid

#173005

Purpose

firefly luciferase reporter vector with 8x clustered TEAD binding sites

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

8xTEAD-luc2

Use

AAV and Luciferase

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-ATG101

Plasmid

#189590

Purpose

To make ATG101/ATG13 HORMA complex

Depositor

James Hurley

Article

Shi et al J Cell Biol. 2020 Jul 6;219(7):e20191104

Insert

ATG101 (ATG101 Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CBA-Con/Fon-EYFP-WPRE

Plasmid

#186752

Purpose

AAV-mediated Cre and Flp-dependent expression of EYFP under CBA promoter

Depositor

Yongling Zhu

Article

Xu et al Cell Rep. 2022 Jul 5;40(1):111036. doi:

Insert

EYFP

Use

AAV

Promoter

CBA

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pNB101

Plasmid

#169110

Purpose

Feedback-controlled AraC circuit

Depositor

Edwin Wintermute

Article

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

araC (araC Escherichia coli K12 MG1655)

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon optimized for E. coli

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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H2A-mTurquoise-RhoJQ79L-deltaCaax

Plasmid

#176110

Purpose

Constitutively active, nucelar localized RhoJ

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

RhoJ (RHOJ Human)

Expression

Mammalian

Mutation

Q79L

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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XXXXA-pHG165c

Plasmid

#170110

Purpose

Constitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the XylS benzoate ligand binding domain.

Depositor

Liskin Swint-Kruse

Article

Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

Insert

XXXXA

Expression

Bacterial

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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Opie2-PuroR[dsxM]

Plasmid

#131616

Purpose

Expresses a functional PuroR only in males and dsRed in both genders.

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

Inserts

PuroR[dsxM] (dsx Fly)
dsRed

Expression

Insect

Promoter

Opie2 and hr5Ie1

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pMH-HT_SsCHAD-H29A-R32A-R36A

Plasmid

#127786

Purpose

Expresses a SsCHAD mutant protein in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

SsCHAD (SULM_RS11045 Sulfolobus solfataricus)

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

mutations in His29, Arg32 and Arg36 to Ala

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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gRNA[DsxF].1026A

Plasmid

#112690

Purpose

express gRNA targeting DsxF under dU6-3 promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

U6.3-gRNA[DsxF] (dsx Fly)

Use

CRISPR

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-PsuACR_353-EYFP

Plasmid

#111076

Purpose

Anion channelrhodopsin from Proteomonas sulcata (CCMP704) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

Insert

Anion channelrhodopsin PsuACR_353

Tags

EYFP

Expression

Mammalian

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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GTT-c

Plasmid

#81108

Purpose

Gene Tagging vector TRP/CFP - Plasmid for yeast enhanced monomeric CFP (yemCFP) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Insert

yemCFP

Use

Cre/Lox

Tags

yemCFP

Expression

Yeast

Available Since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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