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  6. pNOC_dCas9_sgRNA NC

    Plasmid
    #176259
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and spacer sequence on sgRNA is replaced by the type IIS restriction site for endonuclease BaeI and
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Expression
    Mutation
    H840A and D10A mutations on spCas9 to inactivate …
    Promoter
    Available since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    176259-plasmid-map-sequence-id-346581

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  20. pGBT-RP8.1

    Plasmid
    #189846
    Purpose
    Intron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Lens tagBFP frameshift 1.
    Depositor
    Stephen Ekker
    Article
    Ichino et al Elife. 2020 Aug 11;9. pii: 54572. doi: 1
    Type
    Empty backbone
    Use
    Cre/Lox
    Tags
    mRFP and tagBFP
    Expression
    Mutation
    Promoter
    beta Actin
    Available since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    189846-plasmid-map-sequence-id-373953

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Showing: 1901 - 1920 of 3037 results