We narrowed to 17,361 results for: SHE

1. pLVX-EF1alpha-eGFP-2xStrep-IRES-Puro

   Plasmid

   #141395

   Purpose

   Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

   Depositor

   Nevan Krogan

   Article

   Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

   Insert

   eGFP
   

   Use

   Lentiviral

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   EF1alpha

   Available since

   April 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE

   Plasmid

   #131004

   Purpose

   bicistronic AAV vector to express GCaMP6m and soma-targeted ChRmine under the control of human Synapsin promoter

   Depositor

   Karl Deisseroth

   Article

   Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

   Has Service

   AAV8

   Insert

   ChRmine
   

   Use

   AAV

   Tags

   Kv2.1-HA

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti_ABE8e-SpRY-P2A-BFP_HygroR

   Plasmid

   #213009

   Purpose

   A lentiviral vector used to create stable cell lines expressing the ABE8e-SpRY base editor and BFP

   Depositor

   Richard Sherwood

   Article

   Ryu et al Nat Genet. 2024 Apr 24. doi: 10.1038/s41

   Insert

   ABE8e-SpRY-D10A
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A nickase variant of SpRY

   Promoter

   Ef-1a

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. EF1a_Puro_Telo_v2

   Plasmid

   #195139

   Purpose

   ~100 repeats of the human telomere seed sequence fused to the puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to obtain transfectable construct for chromosomal arm loss induction

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   Telomere (RTEL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVX-TetOne-Puro-p21

   Plasmid

   #171122

   Purpose

   Doxycycline inducible expression of p21

   Depositor

   Jason Sheltzer

   Article

   Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

   Insert

   p21 (CDKN1A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-TPH2-Cre

   Plasmid

   #189616

   Purpose

   Expresses Cre recombinase under the control of the tryptophan hydroxylase promoter

   Depositor

   David Clapham

   Article

   Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

   Insert

   Cre Recombinase
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tph2

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-ChRmine-mScarlet-Kv2.1-WPRE

   Plasmid

   #130995

   Purpose

   AAV vector to drive the expression of soma-targeted ChRmine-mScarlet under the control of human synapsin promoter

   Depositor

   Karl Deisseroth

   Article

   Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

   Has Service

   AAV8

   Insert

   ChRmine
   

   Use

   AAV

   Tags

   mScarlet-Kv2.1

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Sept. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR-SIN-BX SpyCatcher003-hCD52

   Plasmid

   #210565

   Purpose

   Expresses Spycatcher003 on the cell surface using human CD52 hinge

   Depositor

   Omer Dushek

   Article

   Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

   Insert

   Spycatcher003 fused to human CD52 hinge
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. lentiTet-LDLR-mCherry BlastR

   Plasmid

   #186739

   Purpose

   Dox inducible LDLR coding sequence expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene

   Depositor

   Richard Sherwood

   Article

   Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

   Insert

   LDLR (LDLR Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. HsTPC2-EYFP

    Plasmid

    #135194

    Purpose

    Expresses tagged TPC2 in mammalian cells

    Depositor

    Antony Galione

    Article

    Davis et al Curr Biol. 2012 Dec 18;22(24):2331-7. do

    Insert

    TPC2 (TPCN2 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pF145 pAcGFP1 SOD1G93A

    Plasmid

    #26406

    Depositor

    Elizabeth Fisher

    Article

    Stevens et al PLoS One. 2010 . 5(3):e9541.

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    G93A

    Promoter

    Available since

    Nov. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pF150 pSOD1G93AAcGFP1

    Plasmid

    #26411

    Purpose

    contains G94A mutation in pSOD1

    Depositor

    Elizabeth Fisher

    Article

    Stevens et al PLoS One. 2010 . 5(3):e9541.

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    G94A

    Promoter

    Available since

    Nov. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. EF1a_Puro_Telo_v1

    Plasmid

    #195138

    Purpose

    ~100 repeats of the human telomere seed sequence fused to the puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to obtain transfectable construct for chromosomal arm loss induction

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    Telomere (RTEL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pENTR221 UBR5 ∆UBR

    Plasmid

    #81063

    Purpose

    Gateway entry vector encoding full length UBR5 W1235L (∆UBR)

    Depositor

    Darren Saunders

    Article

    Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

    Insert

    Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆UBR (UBR5 Human)
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    W1235L (bp g3704t)

    Promoter

    N/A

    Available since

    Jan. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR221 UBR5 ∆HECT

    Plasmid

    #81065

    Purpose

    Gateway entry vector encoding full length UBR5 C2768A (∆HECT)

    Depositor

    Darren Saunders

    Article

    Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

    Insert

    Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆HECT (UBR5 Human)
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    C2768A (bp t8302g and g8303c)

    Promoter

    N/A

    Available since

    Jan. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pB-Tet-On-Arl13b-RhoA sensor

    Plasmid

    #189613

    Purpose

    Tet-On cilia-targeted RhoA sensor (Xlone piggybac back-bone); Arl13b-fused with a sGFP-mScarlet-I based sensor

    Depositor

    David Clapham

    Article

    Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

    Insert

    Arl13b
    

    Use

    Tags

    GFP and mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    Tet3G

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. MmEos3.2

    Plasmid

    #203754

    Purpose

    Encodes the membrane anchor of Src including the first 15 resideues of the N terminus, with 1 myristoylation and short polybasic sequence, with mEos3.2 fused to the C terminus. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    Src15 n-term (SRC Human)
    

    Use

    Tags

    mEos3.2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SHC003BSD-EXOC7

    Plasmid

    #133298

    Purpose

    Expresses EXOC7 in mammalian cells

    Depositor

    Jingshi Shen

    Article

    Wang et al J Biol Chem. 2019 Dec 27;294(52):19988-1

    Insert

    Exocyst complex component 7 (EXOC7 Human)
    

    Use

    Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-Syn1-GRAB-HTR6-cilia

    Plasmid

    #189615

    Purpose

    Expresses cilia-targeted serotonin sensor based on HTR6 receptor and cpEGFP, driven by SYN promoter

    Depositor

    David Clapham

    Article

    Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

    Insert

    HTR6_GRAB
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Syn1

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Chr1q_Cassette-Integration_gRNA

    Plasmid

    #195126

    Purpose

    gRNA in a third generation Cas9 vector with GFP, targeting location downstream of Chr1q_centromere-targeting_gRNA for positive-negative selection cassette integration

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    Chr1q gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge