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Plasmid#166079PurposePlasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RPL22A for double stranded break formation in yeast.DepositorInsertPromoter of RPL22A (RPL22A Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_YBL055C
Plasmid#166077PurposePlasmid for constituive spCas9 and tet-inducible YBL055C targeting sgRNA expression for double stranded break formation in yeastDepositorInsertYBL055C (near PTC3) (YBL055C Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_YNR071C
Plasmid#166081PurposePlasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of YNR071C for double stranded break formation in yeast.DepositorInsertPromoter of YNR071C (YNR071C Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_SLA1
Plasmid#166075PurposePlasmid for constituive spCas9 and tet-inducible SLA1 targeting sgRNA expression for double stranded break formation in yeastDepositorInsertSLA1 (SLA1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_PDR12
Plasmid#166093PurposePlasmid for constituive spCas9 and tet-inducible PDR12 targeting sgRNA expression for double stranded break formation in yeastDepositorInsertPDR12 (PDR12 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_TDH2
Plasmid#166082PurposePlasmid for constituive spCas9 and tet-inducible TDH2 targeting sgRNA expression for double stranded break formation in yeastDepositorInsertTDH2 (TDH2 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_LYS9
Plasmid#166085PurposePlasmid for constituive spCas9 and tet-inducible LYS9 targeting sgRNA expression for double stranded break formation in yeastDepositorInsertLYS9 (LYS9 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
p101_CRISPRai_PiggyBac
Plasmid#213777PurposeExpresses Tet-On inducible CRISPRai orthogonal system with VPR-dSaCas9, dSpCas9-KRAB-BFP, zeocin resistance gene, and Tet-On transactivator. PiggyBac vector.DepositorInsertsVPR-dSaCas9
dSpCas9-KRAB-BFP
zeocin
Tet-On transactivator
UseCRISPRTagsExpressionMutationPromoterEF1a (EF-1-alpha intron a) and Tet_On_TREAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_CUP1-1
Plasmid#166086PurposePlasmid for constituive spCas9 and tet-inducible CUP1-1 targeting sgRNA expression for double stranded break formation in yeastDepositorInsertCUP1-1 (CUP1-1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_TDH3
Plasmid#166084PurposePlasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of TDH3 for double stranded break formation in yeast.DepositorInsertPromoter of TDH3 (TDH3 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_RPL39
Plasmid#166078PurposePlasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RPL39 for double stranded break formation in yeast.DepositorInsertPromoter of RPL39 (Overlaps with 5'UTR and first base of gene) (RPL39 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_YDR344C
Plasmid#166072PurposePlasmid for constituive spCas9 expression and tet-inducible expression of sgRNA targeting intergenic site near HXT6 (within YDR344C) for double stranded break formation in yeast.DepositorInsertIntergenic site near HXT6 (in YDR344C, possible dubious ORF)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
(364) SB KRAB-SadCas9
Plasmid#163022PurposeSleeping Beauty TET-On expression of CRISPRi (without U6-gRNA)DepositorInsertMammalian codon-optimized SadCas9
UseTransposonTagsKRAB and myc NLSExpressionMammalianMutationPromoterTET-OnAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTRE-Tight-BI-Gl NORM-LacZA-TER-LacZB
Plasmid#86194Purposebeta Globin NMD construct with and without PTC under Bi-directional TET on promoterDepositorUseTet onTagsExpressionMammalianMutation39TER in beta Globin in MCS IIPromoterTet onAvailable sinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
(517) SB KRAB-SadCas9 U6-gStop
Plasmid#163023PurposeSleeping Beauty TET-On expression of CRISPRi with gSTOP gRNADepositorInsertMammalian codon-optimized SadCas9
UseTransposonTagsKRAB and myc NLSExpressionMammalianMutationPromoterTet-OnAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPKm-293
Plasmid#105817PurposepcDNA - TET DBD - MTAD. Expresses TET DNA binding domain fused to MTADDepositorInsertTET DBD and MTAD
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSSa31
Plasmid#179504PurposeTet-on genome insertion systemDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterTet-HOP1 (p98CGH)Available sinceFeb. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSSa32
Plasmid#179505PurposeTet-on genome insertion systemDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterTet-HOP1 (p99CGH)Available sinceFeb. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-GCaMP6s
Plasmid#135418PurposeCan be used to drive amplified GCaMP6s expression when combined with TET driver . Can be used to create adeno-associated virus to express TET-driven GCaMP6s.DepositorInsertGCaMP6s
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceFeb. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV TRE3G Neo GFP-Progerin
Plasmid#118710PurposeLentiviral TET-ON inducible GFP-ProgerinDepositorInsertGFP-Progerin (LMNA Human)
UseLentiviral; Tet-on inducibleTagsGFPExpressionMammalianMutationPromoterCMV TRE3G (TET-ON)Available sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only