We narrowed to 7,742 results for: ef1a;
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Plasmid#47458PurposeLITE2.0 TALE-CIB1. Changes from LITE1.0: glycine-serine linker, mutated endogenous NLS, aa318-334 deletion. This particular TALE targeted to Neurog2. EF1-alpha promoter for ubiquitous mammalian exp.DepositorInsertTALE (Neurog2)-GS-CIB1(NLS*,∆318-334)
TagsHAExpressionMammalianPromoterEF1-alphaAvailable SinceOct. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHR_UCOE-EF1a-dCas9-XTEN80-KRAB(Kox1)-IRES-mCherry
Plasmid#188769PurposedCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)DepositorInsertdCas9-XTEN80-KRAB(Kox1)
UseLentiviralTagsHA-2xNLS-XTEN80(linker)-KRAB(Kox1)PromoterEF1aAvailable SinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXR001-mCD4: EF1a-RfxCas13d-P2A-mCD4-T2A-EGFP
Plasmid#228359PurposeRfxCas13d (NLS-RfxCas13d-NLS) with 2A-EGFP and 2A-mCD4 for RNA knockdownDepositorInsertRfxCas13d
UseLentiviralTags2A-EGFP, 2A-mCD4, HA, and NLSExpressionMammalianPromoterEF1AAvailable SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR BFP-guide1
Plasmid#118157PurposeCRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the BFP CDSDepositorInsertKRAB-dCas9-P2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianPromoterEF1a core and U6Available SinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
KZ801: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; EF1a promoter
Plasmid#121814PurposepMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestionDepositorTypeEmpty backboneUseGateway Cloning and Synthetic BiologyAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCVL TLR 3.1 (Sce target) Ef1a iRFP Puro
Plasmid#50417PurposeFluorescent reporter for DNA cleavage/repair in mammalian cells expressing iRFP with selectable puromycin marker.DepositorInsertseGFP with I-Sce I TS
+3 mCherry
UseLentiviralTagsT2A dislinker and iRFPExpressionMammalianMutationembedded I-Sce I TS from 163-185PromoterSFFVAvailable SinceJan. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
KB601: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; EF1a promoter
Plasmid#121819PurposepMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestionDepositorTypeEmpty backboneUseGateway Cloning and Synthetic BiologyAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-C1V1(t/t)-mRuby2-KV2.1-WPRE-SV40
Plasmid#124604PurposeCre-dependent soma-localized C1V1(t/t) for optogenetic stimulationDepositorInsertSoma-localized C1V1(t/t)
UseAAVTagsmRuby2ExpressionMammalianPromoterEf1aAvailable SinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-CoChR-Kv2.1-GP2A-NLS-mScarlet
Plasmid#253383PurposeCre-dependent, soma-targeted CoChR with nuclear mScarlet expressionDepositorInsertst-CoChR-GP2A-NLS-mScarlet
UseAAVExpressionMammalianAvailable SinceMay 15, 2026AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-CoChR-Kv2.1-GP2A-NLS-eGFP
Plasmid#253381PurposeCre-dependent, soma-targeted CoChR with nuclear GFP expressionDepositorInsertst-CoChR-GP2A-NLS-eGFP
UseAAVExpressionMammalianAvailable SinceMay 15, 2026AvailabilityAcademic Institutions and Nonprofits only -
(pRZ75) AAV: U6-gRNA(cs1)-EF1a-Cre-P2A-mCherry
Plasmid#254581PurposeAAV backbone expressing Cre recombinase and mCherry from the EF1a promoter and one U6-driven sgRNA with SapI spacer and capture sequence in scaffoldDepositorInsertCre
UseAAVAvailable SinceMay 13, 2026AvailabilityAcademic Institutions and Nonprofits only -
PB-circBC-UCOE-EF1a-mEGFP(NTag)-IRES-PuroR-WPRE
Plasmid#252243PurposeVIS-seq expression plasmidDepositorTypeEmpty backboneTagsmEGFPExpressionMammalianAvailable SinceMay 12, 2026AvailabilityAcademic Institutions and Nonprofits only -
PB-circBC-UCOE-EF1a-mEGFP(CTag)-IRES-PuroR-WPRE
Plasmid#252244PurposeVIS-seq expression plasmidDepositorTypeEmpty backboneTagsmEGFPExpressionMammalianAvailable SinceMay 12, 2026AvailabilityAcademic Institutions and Nonprofits only -
pPD864 pLP Rosa26 EF1a attP EYFP-P2A-Hygro
Plasmid#253872PurposeContains the landing pad (attP) flanked by Rosa26 homology arms to generate B16F10-LPDepositorInsertattP EYFP-P2A-Hygro
UseSynthetic BiologyExpressionMammalianPromoterEF1aAvailable SinceApril 17, 2026AvailabilityAcademic Institutions and Nonprofits only -
pJC2729 - pLenti-X1-EF1A-HA-FBXO31(∆F-box, D334N)-PGK-Neo
Plasmid#250261PurposecDNA expression of affinity-tagged FBXO31 (HA-FBXO31(∆F-box, D334N)).DepositorInsertFBXO31 (FBXO31 Human)
UseLentiviralTagsHAExpressionMammalianMutation∆F-box, D334NPromoterEF-1aAvailable SinceJan. 20, 2026AvailabilityAcademic Institutions and Nonprofits only -
pJC2735 - pHR-EF1A-HA-FBXO31(∆F-box, D334N)-IRES-GFP
Plasmid#250267PurposecDNA expression of affinity-tagged FBXO31 (HA-FBXO31(∆F-box, D334N)).DepositorInsertFBXO31 (FBXO31 Human)
UseLentiviralTagsHAExpressionMammalianMutation∆F-box, D334NPromoterEF-1aAvailable SinceJan. 15, 2026AvailabilityAcademic Institutions and Nonprofits only -
pJC2733 - pHR-EF1A-HA-FBXO31(∆F-box, T343V)-IRES-GFP
Plasmid#250265PurposecDNA expression of affinity-tagged FBXO31 (HA-FBXO31(∆F-box, T343V)).DepositorInsertFBXO31 (FBXO31 Human)
UseLentiviralTagsHAExpressionMammalianMutation∆F-box, T343VPromoterEF-1aAvailable SinceJan. 15, 2026AvailabilityAcademic Institutions and Nonprofits only -
pJC2738 - pHR-EF1A-DD-3xFLAG-FBXO31(D334N)-IRES-GFP
Plasmid#250269PurposeInducible expression of FBXO31(D334N) with the shield degron system.DepositorInsertFBXO31 (FBXO31 Human)
UseLentiviralTagsDD-3xFLAGExpressionMammalianMutationD334NPromoterEF-1aAvailable SinceJan. 15, 2026AvailabilityAcademic Institutions and Nonprofits only -
LSM0252 (PB)EF1a-FKBP-ADAR2dd-IRESv0-PuroR-P2A-BFP
Plasmid#233443PurposePiggyBac donor plasmid to overexpress FKBP-ADAR2dd (cLIDAR)DepositorInsertFKBP-ADAR2dd-IRESv0-PuroR-P2A-BFP
ExpressionMammalianMutationE488Q, T501A on ADAR2ddPromoterEF1aAvailable SinceJune 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
LSM0258 (PB)EF1a-ADAR2dd-PCP-T2A-PuroR-P2A-BFP
Plasmid#233446PurposePiggyBac donor plasmid to overexpress ADAR2dd-PCPDepositorInsertADAR2dd-PCP-T2A-PuroR-P2A-BFP
ExpressionMammalianMutationE488Q, T501A on ADAR2ddPromoterEF1aAvailable SinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only