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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,128 results for: try

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Showing: 1901 - 1920 of 3128 results

  2. GOSR2(G144W)-mCherry

    Plasmid
    #209887
    Purpose
    Expresses human GOSR2 (GS27) G144W-mutant fused to mChery in mammalian cells
    Depositor
    Geert van den Bogaart
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GOSR2 (GOSR2 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Changed glycine 144 to tryptophan; this mutation …
    Promoter
    CMV
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    209887-plasmid-map-sequence-id-413669

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  3. mILK-L207W-EFGP

    Plasmid
    #176900
    Purpose
    mouse ILK with L207W point mutation cloned into pEGFP-N1
    Depositor
    Sara Wickström
    Publication
    Martin et al Proc Natl Acad Sci U S A. 2022 Mar 15;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Integrin-linked kinase (Ilk Mouse)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Leucine 207 to Tryptophane
    Promoter
    CNV
    Available since
    March 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    176900-plasmid-map-sequence-id-357256

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  4. Epac-S-H170

    Plasmid
    #170352
    Purpose
    mTurq2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Publication
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Insert
    mTurq2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
    Use
    Adenoviral
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Oct. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    170352-plasmid-map-sequence-id-345817

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  14. pcDNA3.1(+)_Nav1.6(K1546TAG, Y371C , T1785P)-HA

    Plasmid
    #239773
    Purpose
    Expresses pathogenic (T1785P) TTX-resistant variant of mouse Nav1.6 with a TAG codon at position 1546 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.
    Depositor
    Ivana Nikić-Spiegel
    Publication
    Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060
    Insert
    Scn8a (Scn8a Mouse)
    Tags
    HA-tag
    Expression
    Mammalian
    Mutation
    K1546TAG, Y371C, T1785P
    Promoter
    CMV
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    239773-plasmid-map-sequence-id-477101

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  17. p3x-Flag Gli3 37R3-2

    Plasmid
    #120305
    Purpose
    Expresses human protein Gli containing a 4HT-inducible intein evolved to operate at 37C
    Depositor
    David Liu
    Publication
    Peck et al Chem Biol. 2011 May 27;18(5):619-30. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Intein 37R3-2, Gli3
    Use
    Synthetic Biology
    Tags
    3X Flag
    Expression
    Mammalian
    Mutation
    Contains mutations as reported in 2011 Chemistry …
    Promoter
    CMV
    Available since
    Feb. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    120305-plasmid-map-sequence-id-234346

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  18. pDual-eGFP(W66)

    Plasmid
    #63217
    Purpose
    Expression of the Celeste (cyan) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.
    Depositor
    Richard S Myers
    Publication
    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1
    Insert
    His-T7-eGFP(W66)
    Use
    Lentiviral
    Tags
    His6 and T7
    Expression
    Bacterial and Mammalian
    Mutation
    Changed tyrosine at position 66 with respect to t…
    Promoter
    CMV-EF1α hybrid (CEF)
    Available since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  19. mutpRL-TK 3xUTR

    Plasmid
    #40758
    Purpose
    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
    Depositor
    Phillip Zamore
    Publication
    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3 copies of the CXCR4 small RNA target site
    Use
    Luciferase
    Tags
    luciferase
    Mutation
    mutated from TAG to CTC at nt 387–389 of the Reni…
    Promoter
    TK
    Available since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 1901 - 1920 of 3128 results