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We narrowed to 3,093 results for: try

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Showing: 1901 - 1920 of 3093 results

  1. pDual-eGFP(W66)

    Plasmid
    #63217
    Purpose
    Expression of the Celeste (cyan) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.
    Depositor
    Richard S Myers
    Publication
    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1
    Insert
    His-T7-eGFP(W66)
    Use
    Lentiviral
    Tags
    His6 and T7
    Expression
    Bacterial and Mammalian
    Mutation
    Changed tyrosine at position 66 with respect to t…
    Promoter
    CMV-EF1α hybrid (CEF)
    Available since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  2. pSems leader-ALFA-mXFP-TpoR

    Plasmid
    #222945
    Purpose
    Expression of TPOR with ALFAtag and monomeric XFP in mammalian cells
    Depositor
    Jacob Piehler
    Publication
    Winkelmann et al Nat Commun. 2024 Jul 10;15(1):5813. doi:
    Insert
    Thrombopoietin receptor (26-635) (MPL Human)
    Tags
    ALFAtag, Ig k-chain leader sequence, and mXFP
    Expression
    Mammalian
    Mutation
    mXFP: tryptophan 66 to phenylalanine, TPOR: Signa…
    Promoter
    CMV
    Available since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    222945-plasmid-map-sequence-id-457424

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  5. pCDNA3.1-nAChr-beta3-CFP

    Plasmid
    #50485
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Publication
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)
    Tags
    CFP
    Expression
    Mammalian
    Mutation
    CFP fusion in M3-M4 loop (after residue P379). O…
    Promoter
    CMV
    Available since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  6. pACYC-T7-SpCas9(BspMI/SapI/BsaI_cassettes)-T7-gRNA1 (BPK1807)

    Plasmid
    #223065
    Purpose
    Dual pT7 entry plasmid for SpCas9(6AA) library; bacterial expression plasmid with type IIS RE cassettes around D1135/S1136, G1218/E1219, R1335/T1337 (precursor to MMW94). Expresses a gRNA.
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    entry vector for human codon opt. bacterial expr. plasmid for SpCas9(6AA_NNS) library, with gRNA targeting EGFP site 1
    Use
    CRISPR
    Tags
    NLS(SV40)-3xFLAG
    Expression
    Bacterial
    Mutation
    Three regions of SpCas9 encode type IIS restricti…
    Promoter
    Dual T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223065-plasmid-map-sequence-id-447614

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  7. pME-mCherry-PA-Rac1

    Plasmid
    #169042
    Purpose
    Middle entry vector for Gateway cloning that contains an mCherry fused with an optimized photoactivatable Rac1 gene insert
    Depositor
    Paola Arlotta
    Publication
    Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d
    Insert
    PA-Rac-1 (RAC1 Avena sativa (oat), Human)
    Use
    Gateway entry cloning vector
    Tags
    6X His and Fused with mCherry
    Mutation
    Rac1 starts at I4 and contains mutations Q61L, E9…
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    169042-plasmid-map-sequence-id-341784

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  8. PF3D7_1012200-COMP-blac-flag-his

    Plasmid
    #111017
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Publication
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    rhoptry associated adhesin (PF3D7_1012200 Synthetic)
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Oct. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    111017-plasmid-map-sequence-id-224584

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  9. pTcTurboID-GW

    Plasmid
    #234714
    Purpose
    Used to express any CDS fused to 3xHA-TurboID (N-terminal) in T. cruzi. Tetracycline regulated. Modified from Alonso et al. (2014).
    Depositor
    Esteban Serra
    Type
    Empty backbone
    Use
    Trypanosomatid expression
    Tags
    3xHA
    Available since
    May 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    234714-plasmid-map-sequence-id-473963

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  10. ZM2

    Plasmid
    #32784
    Depositor
    George Cross
    Insert
    T7rnap
    Use
    Cre/Lox; Floxed targeting vector for trypanosome
    Available since
    Dec. 13, 2011
    Availability
    Academic Institutions and Nonprofits only

  12. pTcTurboID-GW_GFP

    Plasmid
    #234715
    Purpose
    Used to express GFP fused to 3xHA-TurboID (N-terminal) in T. cruzi. Tetracycline regulated. Used as proximity labelling cytoplasmatic spatial control. CDS can be cloned in frame to GFP using EcoRV.
    Depositor
    Esteban Serra
    Insert
    GFP
    Use
    Trypanosomatid expression
    Tags
    3xHA
    Promoter
    T7
    Available since
    May 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    234715-plasmid-map-sequence-id-473962

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  17. pTcTurboID-GW_GFP-NLS

    Plasmid
    #234716
    Purpose
    Used to express GFP-3xNLS fused to 3xHA-TurboID (N-terminal) in T. cruzi. Tetracycline regulated. Used as proximity labelling nuclear spatial control.
    Depositor
    Esteban Serra
    Insert
    GFP-3xNLS
    Use
    Trypanosomatid expression
    Tags
    3xHA
    Promoter
    T7
    Available since
    May 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    234716-plasmid-map-sequence-id-473540

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  18. pJBL7042

    Plasmid
    #167213
    Purpose
    overexpression of MerR in E. coli for cell-free mercury sensing
    Depositor
    Julius Lucks
    Insert
    MerR
    Expression
    Bacterial
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    167213-plasmid-map-sequence-id-330998

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  19. pJBL7047

    Plasmid
    #167217
    Purpose
    overexpression of CadR in E. coli for cell-free cadmium sensing
    Depositor
    Julius Lucks
    Insert
    CadR
    Expression
    Bacterial
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    167217-plasmid-map-sequence-id-331011

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Showing: 1901 - 1920 of 3093 results