We narrowed to 17,224 results for: emb

1. BS-ZJLEU-PTEF1-HFL1-D384A-GFP

   Plasmid

   #207021

   Purpose

   Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   HFL1
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   D384A

   Promoter

   pTEF1

   Available since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. BS-ZJLEU-PTEF1-HFL1-I375A-GFP

   Plasmid

   #207020

   Purpose

   Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   HFL1
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   I375A

   Promoter

   pTEF1

   Available since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. BS-ZJLEU-PTEF1-HFL1-W371A-GFP

   Plasmid

   #207019

   Purpose

   Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   HFL1
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   W371A

   Promoter

   pTEF1

   Available since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. ClhN-P1K-3VSV-Atg8-MET15

   Plasmid

   #207055

   Purpose

   Expression of 3VSV-Atg8. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   ATG8
   

   Use

   Tags

   3xVSV

   Expression

   Yeast

   Mutation

   Promoter

   pATG8

   Available since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. P1K-3HA-Atg8∆R-MET15

   Plasmid

   #207034

   Purpose

   Expression of 3HA-Atg8∆R. This variant bypasses the need of Atg4 for initial C-terminal processing.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   ATG8
   

   Use

   Tags

   3xHA

   Expression

   Yeast

   Mutation

   ∆R

   Promoter

   pATG8

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYea5.1_Notch1-L24_sfGFP

   Plasmid

   #207865

   Purpose

   Yeast 2µ vector for expression of Suc2SP-Notch1-L24_100-sfGFP-GAL4

   Depositor

   Dieter Langosch

   Article

   Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

   Insert

   Suc2SP-Notch1-L24_100-sfGFP-GAL4 fusion protein
   

   Use

   Tags

   Strep-TagII

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYea5.1_Notch1_sfGFP

   Plasmid

   #207801

   Purpose

   Yeast 2µ vector for expression of Suc2SP-Notch1_100-sfGFP-GAL4

   Depositor

   Dieter Langosch

   Article

   Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

   Insert

   Suc2SP-Notch1_100-sfGFP-GAL4 fusion protein
   

   Use

   Tags

   Strep-TagII

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   TEF

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSEVA424::tsi5

   Plasmid

   #192960

   Purpose

   Expresses the protein Tsi51-76 for biological function studies in a broad-spectrum of organisms

   Depositor

   David Albesa-Jové

   Article

   Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

   Insert

   tsi5
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET29a(+)::D1141A

   Plasmid

   #192963

   Purpose

   Test the activity of the putative aspartyl protease motif DPXGL-(18)-DPXGL

   Depositor

   David Albesa-Jové

   Article

   Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

   Insert

   Tse5_D1141A
   

   Use

   Tags

   9xhis + TEV tag

   Expression

   Bacterial

   Mutation

   D1141A

   Promoter

   Available since

   Dec. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET29a(+)::9xhis-Tse5

    Plasmid

    #192962

    Purpose

    Heterologous expression of Tse5 in E. coli Lemo21 cells for purification of Tse5-CT

    Depositor

    David Albesa-Jové

    Article

    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

    Insert

    Tse5
    

    Use

    Tags

    9xhis + TEV tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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