We narrowed to 32,235 results for: uros

1. pSpCas9-Puro HLAG-2B-sgRNA

   Plasmid

   #182552

   Purpose

   Cas9 from S.pyogenes with 2A-Puro, and the 2B-sgRNA to targeting exon 2 of human HLA-G gene

   Depositor

   Santiago Miriuka

   Article

   Palma et al Sci Rep. 2021 Nov 12;11(1):22158. doi: 1

   Insert

   hSpCas9-2A-Puro-HLAG-2B-sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSpCas9(BB)-2A-Puro-V2.0-sgHsAGO3_AH

   Plasmid

   #148855

   Purpose

   Mammalian Expression of HsAGO3-sgRNA

   Depositor

   Elisa Izaurralde

   Insert

   HsAGO3-sgRNA (AGO3 S.pyogenes)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-AuroraB L154G/H250Y

   Plasmid

   #108492

   Purpose

   expression of EGFP-AuroraB Analog sensitive (L154G/H250Y)

   Depositor

   Susanne Lens

   Article

   Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

   Insert

   AuroraB (AURKB Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Analog sensitive L154G/H250Y

   Promoter

   CMV

   Available Since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-VQRRA-P2A-Puro

   Plasmid

   #110851

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VQRRA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VQRRA
   

   Use

   Lentiviral

   Tags

   FLAG

   Mutation

   D1135V, R1335Q, T1337R and NLS sequence at the N-…

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO.1-puro U6 sgRNA SOX17 -91

   Plasmid

   #50923

   Purpose

   U6 driven sgRNA targeting Sox17 -91 bp from TSS

   Depositor

   Scot Wolfe, Rene Maehr

   Article

   Kearns et al Development. 2014 Jan;141(1):219-23. doi

   Insert

   Sox17 -91 sgRNA (SOX17 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. FLAG-EglN2 wt-pBabe-Puro

   Plasmid

   #22704

   Depositor

   William Kaelin

   Article

   Zhang et al Cancer Cell. 2009 Nov 6. 16(5):413-24.

   Insert

   Egg laying Nine - 2 (EglN2) (EGLN2 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   wt cDNA

   Available Since

   Dec. 3, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVX-UBE3B-BirA-Puro

   Plasmid

   #107352

   Purpose

   Expresses UBE3B fused to BirA in mammalian cells.

   Depositor

   Robert Sobol

   Article

   Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

   Insert

   UBE3B-BirA (UBE3B Human)
   

   Use

   Lentiviral

   Tags

   Biotin ligase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 23, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pSUPER-retro-puro-Smad2

   Plasmid

   #89827

   Purpose

   Knockdown human Smad2 expression in human mammalian cells

   Depositor

   Shengyu Yang

   Article

   Sun et al J Biol Chem. 2011 Nov 11;286(45):38865-7

   Insert

   SMAD2 shRNA (SMAD2 Human)
   

   Use

   Retroviral

   Available Since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSUPER-retro-puro-shNup133-1

   Plasmid

   #87334

   Purpose

   To express shRNA against human Nup133

   Depositor

   Lei Lu

   Article

   Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

   Insert

   shRNA against human Nup133
   

   Use

   RNAi

   Expression

   Mammalian

   Promoter

   H1

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCR3-Flag-AuroraB KD (K106R)

    Plasmid

    #108488

    Purpose

    expression of Flag-AuroraB kinase death (K106R)

    Depositor

    Susanne Lens

    Article

    Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

    Insert

    AuroraB (AURKB Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Kinase death K106A

    Promoter

    CMV

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDH-puro/Flag PD-L1 N35Q

    Plasmid

    #121449

    Purpose

    expression of human PD-L1 mutant in mammalian cells

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    CD274 (CD274 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    N35Q

    Promoter

    CMV

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. miniTol2 x EF1a_EKAREN4 + PGK-puro

    Plasmid

    #167824

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN4
    

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKF-D2irTNP2AG-T2APuroR-5kwh

    Plasmid

    #128255

    Purpose

    Mammalian linearizer gene circuit based on negative feedback in a Flp-In expression system controlling the PuroR gene.

    Depositor

    Gabor Balazsi

    Article

    Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

    Insert

    hTetR::P2A::EGFP::T2A::PuroR
    

    Use

    Synthetic Biology; Flp-in expression vector

    Expression

    Mammalian

    Promoter

    CMV-D2ir promoter

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDEST-IRES-puro-polyA

    Plasmid

    #43967

    Depositor

    Peter Hohenstein

    Article

    Dolt et al PLoS One. 2013 Apr 29;8(4):e62054. doi:

    Insert

    puroR
    

    Use

    Gateway dest vector

    Available Since

    Sept. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMXs-Puro-SNX2-PRDM6

    Plasmid

    #69470

    Purpose

    Expresses SNX2-PRDM6 fusion gene

    Depositor

    Axel Hillmer

    Article

    Yao et al Cell Rep. 2015 Jul 14;12(2):272-85. doi:

    Insert

    SNX2-PRDM6 (SNX2, PRDM6 Human)
    

    Use

    Retroviral

    Tags

    HA TAG

    Available Since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. TET-pLKO.1 PURO shId2 #2

    Plasmid

    #83090

    Purpose

    Lentiviral shRNA vector for inducible knockdown of mouse Id2

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    shId2 (Id2 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

17. pMKO.1-puro/hFOXH1 shRNA1

    Plasmid

    #59303

    Purpose

    Knockdown of human FOXH1

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Nat Commun. 2014 Apr 24;5:3678. doi: 10.

    Insert

    hFOXH1 shRNA (FOXH1 Human)
    

    Use

    RNAi and Retroviral

    Promoter

    hU6

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pTet-O-APOE-E4-T2A-Puro

    Plasmid

    #190813

    Purpose

    Express APOE E4 allele from tet-inducible promoter, co-expressing Puro resistance gene

    Depositor

    Ronald Hart

    Insert

    APOE (APOE Human)
    

    Use

    Lentiviral

    Mutation

    E4

    Promoter

    Tet-O

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTet-O-APOE-E3-T2A-Puro

    Plasmid

    #190812

    Purpose

    Express APOE E3 allele from tet-inducible promoter, co-expressing Puro resistance gene

    Depositor

    Ronald Hart

    Insert

    APOE (APOE Human)
    

    Use

    Lentiviral

    Mutation

    E3

    Promoter

    Tet-O

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTet-O-APOE-E2-T2A-Puro

    Plasmid

    #190811

    Purpose

    Express APOE E2 allele from tet-inducible promoter, co-expressing Puro resistance gene

    Depositor

    Ronald Hart

    Insert

    APOE (APOE Human)
    

    Use

    Lentiviral

    Mutation

    E2

    Promoter

    Tet-O

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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