We narrowed to 2,545 results for: crispr system

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pTol1-U6abc_ect2_cmlc2-nmKate

Plasmid

#238410

Purpose

Drives expression of 3 different gRNAs targeting ect2, and expression of nuclear mKate in cardiomyocytes

Depositor

Juan Manuel González-Rosa

Article

Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

Insert

nuclear mKate/3 gRNAs targeting ect2

Promoter

cmlc2 (nmKate); U6 (gRNAs)

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-sgRNA_SRD5A1-mcherry

Plasmid

#159780

Purpose

S. pyogenes sgRNA collocated with pegRNA targeting human SRD5A1 gene

Depositor

Xiaolong Wang

Article

Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

Insert

spacer of sgRNA targeting SRD5A1 gene (SRD5A1 Human)

Expression

Mammalian

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pRS315e_pGal-dCas9-SH3

Plasmid

#79613

Purpose

Expresses dCas9-SH3 in yeast cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Tags

SH3 domain

Expression

Yeast

Mutation

D10A and H840A for nuclease deficient Cas9

Promoter

pGal1

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pRP_8xgRNA-FLuc

Plasmid

#135937

Purpose

Firefly luciferase reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activation.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

8 copies of gRNA binding site (5'-AAAGGTCGAGAAACTGCAAA-3')

Use

Luciferase

Expression

Mammalian

Promoter

minCMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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Px-PhiYFP-triplex-28-M13-28-pA

Plasmid

#202047

Purpose

Encodes PhiYFP downstream of a cloning site for constructing a synthetic Cas-targeted promoter. 3’ UTR has a MALAT1 triplex, Csy4 28nt sites, and cloning site for gRNA to activate downstream targets.

Depositor

Darren Cusanovich

Article

Kang et al ACS Synth Biol. 2025 May 16;14(5):1732-1

Insert

PhiYFP

Expression

Mammalian

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-Triplex-28-M13-28-pA

Plasmid

#202041

Purpose

Encodes a non-coding transcript expressed from the EF1a promoter that has a 3' UTR containing a MALAT1 triplex, Csy4 binding sites ("28"), and a cloning site for a Cas9 gRNA.

Depositor

Darren Cusanovich

Article

Kang et al ACS Synth Biol. 2025 May 16;14(5):1732-1

Insert

Triplex-28-M13-28 (noncoding)

Expression

Mammalian

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Px-PhiYFP-triplex-DR-M13-DR-pA

Plasmid

#202046

Purpose

Encodes PhiYFP downstream of a cloning site for constructing a synthetic Cas-targeted promoter. 3’ UTR has a MALAT1 triplex, dCas12a DRs, and cloning site for crRNA to activate downstream targets.

Depositor

Darren Cusanovich

Article

Kang et al ACS Synth Biol. 2025 May 16;14(5):1732-1

Insert

PhiYFP

Expression

Mammalian

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pTK73_htz-1

Plasmid

#174546

Purpose

htz-1 targeting gRNA expression

Depositor

Masato Kanemaki

Article

Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

Insert

htz-1 targeting gRNA

Expression

Worm

Promoter

Ce-U6 promoter

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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HR700_HA2TP53_Ex5

Plasmid

#228860

Purpose

Backbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 5

Depositor

Thorsten Stiewe

Article

Funk et al Nat Genet. 2025 Jan;57(1):140-153. doi:

Type

Empty backbone

Use

CRISPR and Cre/Lox

Expression

Mammalian

Available Since

Feb. 4, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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HR700_HA2TP53_Ex6

Plasmid

#228861

Purpose

Backbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 6

Depositor

Thorsten Stiewe

Article

Funk et al Nat Genet. 2025 Jan;57(1):140-153. doi:

Type

Empty backbone

Use

CRISPR and Cre/Lox

Expression

Mammalian

Available Since

Feb. 4, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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HR700_HA2TP53_Ex7

Plasmid

#228862

Purpose

Backbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 7

Depositor

Thorsten Stiewe

Article

Funk et al Nat Genet. 2025 Jan;57(1):140-153. doi:

Type

Empty backbone

Use

CRISPR and Cre/Lox

Expression

Mammalian

Available Since

Feb. 4, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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HR700_HA2TP53_Ex8

Plasmid

#228863

Purpose

Backbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 8

Depositor

Thorsten Stiewe

Article

Funk et al Nat Genet. 2025 Jan;57(1):140-153. doi:

Type

Empty backbone

Use

CRISPR and Cre/Lox

Expression

Mammalian

Available Since

Feb. 4, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pPlatTET-gRNA2

Plasmid

#82559

Purpose

All in one vector which contains Cas9 peptide array (linker length: 22aa), antibody-sfGFP-TET1CD, and gRNA expression system.

Depositor

Izuho Hatada

Article

Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

Insert

dCas9-5xPlat2AflD-P2A-scFvGCN4sfGFPTET1CD

Expression

Mammalian

Available Since

Sept. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pSLQ2813 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE PGK-ABI-tagBFP-SpdCas9

Plasmid

#84242

Purpose

Expresses GA-inducible KRAB-Sp dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

GAI-tagBFP-Sp dCas9
GID1-KRAB

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), GAI, GID1, HA tag, IRES-Puro, and t…

Expression

Mammalian

Promoter

CAG and PGK

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pSH231-EFS-Cas9-BlastR

Plasmid

#115146

Purpose

Safe harbor site 231 knock-in vector with Cas9-BlastR expression cassette

Depositor

Raymond Monnat, Michael Phelps

Article

Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

Insert

Cas9-T2A-BlastR

Use

CRISPR

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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S5_EcoRV_Ef1a_AscI

Plasmid

#128469

Purpose

Ef1a promoter

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

Ef1a promoter

Expression

Bacterial

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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S13_EcoRV_UBC_AscI

Plasmid

#128477

Purpose

UBC promoter

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

UBC promoter

Expression

Bacterial

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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S6_BstBI_SV40_SwaI

Plasmid

#128470

Purpose

SV40 promoter

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

SV40 promoter

Expression

Bacterial

Available Since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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S12_EcoRV_PGK_AscI

Plasmid

#128476

Purpose

PGK promoter

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

PGK promoter

Expression

Bacterial

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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S11_EcoRV_CAMKIIA_AscI

Plasmid

#128475

Purpose

CAMKII1 promoter

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

CAMKII1 promoter

Expression

Bacterial

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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