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BCTV-VS domesticated-pUPD2

Plasmid

#240748

Purpose

Domesticated BCTV virion sense ORFs with A1-B1 Goldenbraid grammar

Depositor

Jose Alonso

Publication

Neubauer et al Plant Biotechnol J. 2025 Sep 21. doi: 10

Insert

BCTV VS

Use

Synthetic Biology

Mutation

2nt substitution in V3

Available since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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BCTVΔREP-pUPD2

Plasmid

#240715

Purpose

BCTV complementary sense ORFs with mutated Rep with GoldenBraid C1 grammar

Depositor

Jose Alonso

Publication

Neubauer et al Plant Biotechnol J. 2025 Sep 21. doi: 10

Insert

BCTV CS

Use

Synthetic Biology

Mutation

2 stop codons inserted in Rep

Available since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pCODE-3

Plasmid

#247475

Purpose

Plasmid for inducible expression of six tRNA encoding genes (argX, glyT, leuW, proL, argU, and ileX) in Escherichia coli, based on pSEVA121 backbone

Depositor

Morten Norholm

Publication

Feller et al ACS Synth Biol. 2026 Apr 21. doi: 10.102

Insert

argX, glyT, leuW, proL, argU, and ileX

Expression

Bacterial

Mutation

Rearrangement of tRNA operon, tRNA flanking regio…

Promoter

pTet

Available since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-3HA-TBC1D30

Plasmid

#246269

Purpose

Rab8 GAP

Depositor

Ryohei Yasuda

Publication

Wang et al Elife. 2025 Sep 23;13:RP103879. doi: 10.

Insert

3HA-TBC1D30 (Tbc1d30 Rat)

Tags

3HA

Expression

Mammalian

Promoter

CAG

Available since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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BtA3N

Plasmid

#238981

Purpose

In vitro transcription of bovine arrestin-3 short isoform

Depositor

Vsevolod Gurevich

Publication

Ahmed et al Biochemistry. 2011 May 10;50(18):3749-63

Insert

arrestin-3 short (ARR3 Bovine)

Use

In vitro transcription

Mutation

silent, Cla I side introduced before stop codon

Promoter

SP6

Available since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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pISH-Olfr1508

Plasmid

#136746

Purpose

Template to synthesize riboprobes for in situ hybridization (ISH) for Olfr1508

Depositor

Peter Mombaerts

Publication

Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

Insert

Olfr1508 (Or4e1 Mouse)

Use

In situ probe

Mutation

ISH Probe insert

Available since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pISH-Olfr288

Plasmid

#136749

Purpose

Template to synthesize riboprobes for in situ hybridization (ISH) for Olfr288

Depositor

Peter Mombaerts

Publication

Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

Insert

Olfr288 (Olfr288 Mouse)

Use

In situ probe

Mutation

ISH Probe insert

Available since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pISH-Olfr1395

Plasmid

#136731

Purpose

Template to synthesize riboprobes for in situ hybridization (ISH) for Olfr1395

Depositor

Peter Mombaerts

Publication

Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

Insert

Olfr1395

Use

In situ probe

Mutation

ISH Probe insert, D175G and L220F- please dep. co…

Available since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pISH-Vmn2r120-TR

Plasmid

#108087

Purpose

Preparation of RNA probe for ISH of mouse Vmn2r120 gene

Depositor

Peter Mombaerts

Publication

Akiyoshi et al Eur J Neurosci. 2018 Apr;47(7):887-900.

Insert

Vmn2r120 (Vmn2r120 Mouse)

Use

In vitro transcription

Mutation

changed A to G at nt 1196 of NM_001104591.1

Available since

May 17, 2019

Availability

Academic Institutions and Nonprofits only

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pBW301ParsR-Amp30T

Plasmid

#78653

Purpose

pSB3K3 carrying araC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-30hrpR-30hrpS-t-hrpL-30gfp-t

Depositor

Baojun Wang

Publication

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

araC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-

Use

Synthetic Biology

Promoter

see insert

Available since

Aug. 1, 2016

Availability

Academic Institutions and Nonprofits only
pBP-ORF-mCherry

Plasmid

#72962

Purpose

Level 0 Entry vector" ORF - mCherry "

Depositor

Paul Freemont

Publication

Moore et al ACS Synth Biol. 2016 May 2.

Insert

mCherry fluorescent protein

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pMyb12 (GB0075)

Plasmid

#68199

Purpose

Provides the CDS of theSolanum lycopersicum Myb12 transcription factor as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Myb12 coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

Oct. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pLexAOp (GB0118)

Plasmid

#68181

Purpose

Provides the 4xLexA Operator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

4xOpLexA

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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C233

Plasmid

#174349

Purpose

Template for making mRNA for overexpressing frog PABPC1 M161A mutant (C-FLAG) in frog oocytes

Depositor

David Bartel

Publication

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

PABPC1 (M161A) (pabpc1.S Frog)

Use

In vitro transcription template

Tags

FLAG

Mutation

M161A

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mEGFP-Rab4a(Q72L)

Plasmid

#246252

Purpose

Rab4a (Constitutively active)

Depositor

Ryohei Yasuda

Publication

Wang et al Elife. 2025 Sep 23;13:RP103879. doi: 10.

Insert

mEGFP-Rab4a(Q72L) (Rabggtb Rat)

Expression

Mammalian

Mutation

Q72L

Promoter

CAG

Available since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-3HA-Rab3ip

Plasmid

#246268

Purpose

Rab8 GAP

Depositor

Ryohei Yasuda

Publication

Wang et al Elife. 2025 Sep 23;13:RP103879. doi: 10.

Insert

3HA-Rab3ip (Rab3ip Rat)

Tags

3HA

Expression

Mammalian

Promoter

CAG

Available since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFP-Akt2(K14A/R25E)-Halo-PS

Plasmid

#86632

Purpose

Expresses eGFP-Akt2-HaloTag-PS fusion (cytosolic tandem)

Depositor

Thomas Leonard, Ivan Yudushkin

Publication

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt2(K14A/R25E)-Halo-PS (AKT2 )

Expression

Mammalian

Available since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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pTU2_2b

Plasmid

#73011

Purpose

Secondary module (BpiI) site in pTU2b backbone

Depositor

Paul Freemont

Publication

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Secondary module + Golden Gate pTU2b BsmBI destination site

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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BCTVΔREP-YPET-pDGB3alpha1

Plasmid

#240725

Purpose

BCTV vector for YPET expression. Rep has been mutated.

Depositor

Jose Alonso

Publication

Neubauer et al Plant Biotechnol J. 2025 Sep 21. doi: 10

Insert

YPET

Expression

Plant

Mutation

2 stop codons inserted in Rep

Available since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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BCTVΔVSΔREP-YPET-pDGB3alpha1

Plasmid

#240726

Purpose

BCTV vector for YPET expression. Lacks the BCTV virion sense ORFs and Rep has been mutated.

Depositor

Jose Alonso

Publication

Neubauer et al Plant Biotechnol J. 2025 Sep 21. doi: 10

Insert

YPET

Expression

Plant

Mutation

2 stop codons inserted in Rep

Available since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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