We narrowed to 8,657 results for: pcDNA3

1. pcDNA3-mCherry-Rheb(5A)

   Plasmid

   #192439

   Purpose

   Encodes mCherry-tagged Rheb lacking residues 38-42.

   Depositor

   Jin Zhang

   Article

   Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

   Insert

   mCherry-Rheb(C181S) (RHEB Human)
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Rheb amino acids 38-42 deleted.

   Promoter

   CMV

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-Myc:P4:gs:nls:ABI

   Plasmid

   #135985

   Purpose

   Expresses the P4 coiled coil fused to the ABI abscisic acid-inducible domain in mammalian cells

   Depositor

   Roman Jerala

   Article

   Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

   Insert

   P4 coiled-coil fused to the ABI domain
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. HA-UBE2E1 C131A in pcDNA3.1

   Plasmid

   #118214

   Purpose

   Expresses HA-UBE2E1 C131A catalytic mutant in mammalian cells

   Depositor

   Cynthia Wolberger

   Article

   Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

   Insert

   UBE2E1 (UBE2E1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   changed cysteine 131 to alanine

   Available Since

   Oct. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcdna3 Flag JIP3b

   Plasmid

   #53458

   Purpose

   interacts with MAPK signaling module

   Depositor

   Roger Davis

   Article

   Kelkar et al Mol Cell Biol. 2000 Feb;20(3):1030-43.

   Insert

   mitogen-activated protein kinase 8 interacting protein 3 (Mapk8ip3 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Sept. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3-DAKAP-μDpTT

   Plasmid

   #182324

   Purpose

   Mitochondria-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

   Depositor

   Jin Zhang

   Article

   Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

   Insert

   DAKAP-μDronpa-TagRFP-T
   

   Tags

   N-terminal 30 amino acids from DAKAP1

   Expression

   Mammalian

   Mutation

   Dronpa contains H193T mutation.

   Promoter

   CMV

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-CBX1mut-PA-mCherry

   Plasmid

   #138251

   Purpose

   Triple-mutant CBX1 chromodomain (residues 20-73) tagged with photoactivatable mCherry (PAmCherry). Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1.

   Depositor

   Jin Zhang

   Article

   Hard et al Sci Adv. 2018 Nov 7;4(11):eaau1447. doi:

   Insert

   CBX1mut-PAmCherry
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-Nrxn1beta AS4(-)-Fc

   Plasmid

   #59313

   Purpose

   Expression plasmid for secreted Fc-fusion of mouse Neurexin 1beta AS4(-) [lacking the insert at alternatively spliced segment 4 (AS4-)]

   Depositor

   Tito Serafini, Peter Scheiffele

   Article

   Scheiffele et al Cell. 2000 Jun 9. 101(6):657-69.

   Insert

   Nrxn1beta AS4(-)-Fc fusion (Nrxn1 Mouse)
   

   Tags

   Fc (from human IgG)

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. Cav2.2 BBS pcDNA3

   Plasmid

   #206057

   Purpose

   expression of rabbit Cav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain II

   Depositor

   Annette Dolphin

   Article

   Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3 ER-Ras-LOCKR-S

   Plasmid

   #216500

   Purpose

   FRET-based reporter for Ras activity, ER localized

   Depositor

   David Baker

   Article

   Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

   Insert

   ER-Ras-LOCKR-S
   

   Expression

   Mammalian

   Available Since

   April 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 MYC dHLH

    Plasmid

    #176047

    Purpose

    Expresses Myc Mutant (delta helix-hoop-helix) in Mammalian cells

    Depositor

    Linda Penn

    Article

    Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

    Insert

    Myc
    

    Expression

    Mammalian

    Mutation

    deleted amino acids 371-412

    Promoter

    CMV

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-sACE2v2.4(732)-8h

    Plasmid

    #154103

    Purpose

    Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2.4 (8his tag)

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Tags

    Short GSG linker and 8his tag

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-G…

    Promoter

    CMV

    Available Since

    June 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3puro-mGFP-TOP3B

    Plasmid

    #225470

    Purpose

    Express mEGFP-fusion protein of TOP3B

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    TOP3B (TOP3B Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-delta133HtrA-EGFP

    Plasmid

    #14124

    Depositor

    L. Miguel Martins

    Article

    Martins et al J Biol Chem. 2002 Jan 4. 277(1):439-44.

    Insert

    HtrA2 (HTRA2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deletion of the first 133 amino-acids

    Available Since

    March 7, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pCDNA3-myc3-FBW5

    Plasmid

    #19905

    Depositor

    Yue Xiong

    Article

    Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

    Insert

    F-box and WD repeat domain containing 5 (FBXW5 Human)
    

    Tags

    myc3

    Expression

    Mammalian

    Available Since

    April 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pY111 (pcDNA3.1-huTsCpf1)

    Plasmid

    #92267

    Purpose

    Expression of humanized TsCpf1

    Depositor

    Feng Zhang

    Insert

    huTsCpf1
    

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Available Since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1_mGFP-FGFR3-K650Q

    Plasmid

    #191756

    Purpose

    Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-K650Q mutation (associated with HCH; target mutation: c.1948A>C; silent mutation added on purpose: c.1938C>T)

    Depositor

    Irene Tiemann-Boege

    Article

    Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

    Insert

    mGFP-FGFR3IIIc
    

    Tags

    mGFP

    Expression

    Mammalian

    Mutation

    c.1948A>C (p.K650Q) + silent mutation c.1938C&…

    Available Since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-NPY-ClopHensor

    Plasmid

    #25939

    Depositor

    Daniele Arosio

    Article

    Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

    Insert

    NPY-ClopHensor (NPY Aq. Victoria and Discosoma sp)
    

    Tags

    NPY and Strep-II

    Expression

    Mammalian

    Mutation

    Neuropeptide Y localization signal

    Available Since

    Sept. 13, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-ccdB-Renilla

    Plasmid

    #87065

    Purpose

    Mammalian Gateway-compatible expression vector backbone with a C-terminal Renilla luciferase

    Depositor

    Mikko Taipale

    Type

    Empty backbone

    Tags

    Renilla luciferase

    Expression

    Mammalian

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-TIE-V5/HIS

    Plasmid

    #205612

    Purpose

    Expression of human TIE receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    TIE receptor tyrosine kinase (TIE1 Human)
    

    Tags

    V5-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-HA-ERK2 D319N

    Plasmid

    #8979

    Depositor

    John Blenis

    Article

    Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

    Insert

    ERK2 (Mapk1 Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D319N

    Available Since

    Oct. 11, 2005

    Availability

    Academic Institutions and Nonprofits only