We narrowed to 5,909 results for: org

1. Tol2 8xSTAR-mNeonGreen-NLS. PGK-puro

   Plasmid

   #136262

   Purpose

   Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

   Depositor

   Hugo Snippert

   Article

   Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

   Insert

   stem cell Ascl2 reporter, 8 repeats
   

   Use

   Tol2 transposase

   Tags

   mNeonGreen-NLS

   Expression

   Mammalian

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pASPIre2

   Plasmid

   #154843

   Purpose

   Derivative of pASPIre1 carrying a bxb1-sfGFP translational fusion in place of bxb1

   Depositor

   Markus Jeschek

   Article

   Hollerer et al Nat Commun. 2020 Jul 15;11(1):3551. doi:

   Insert

   Bxb1-sfGFP fusion controlled by rhamnose-inducible promoter; attB/attP-flanked discriminator containing mCherry; rhaRS operon
   

   Expression

   Bacterial

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Tol2 8xSTAR-sTomato-NLS. PGK-puro

   Plasmid

   #136261

   Purpose

   Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

   Depositor

   Hugo Snippert

   Article

   Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

   Insert

   stem cell Ascl2 reporter, 8 repeats
   

   Use

   Tol2 transposase

   Tags

   sTomato-NLS

   Expression

   Mammalian

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET22b_PstS_1

   Plasmid

   #78198

   Purpose

   Expression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor

   Depositor

   Martin Webb

   Article

   Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

   Insert

   pstS (A197C)
   

   Expression

   Bacterial

   Mutation

   A197C (please see depositors comment below)

   Promoter

   T7

   Available Since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. Str-KDEL_ManII-SBP-mCherry

   Plasmid

   #65253

   Purpose

   synchronize trafficking of ManII from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Streptavidin-KDEL and truncated Mannosidase II
   

   Tags

   Streptavidin Binding Protein (SBP) and mCherry

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 116 of ManII

   Promoter

   CMV

   Available Since

   June 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pfa6a PhoCl-TsCC(B)::KANMX

   Plasmid

   #185906

   Purpose

   Plasmid for cloning PhoCl fused to TsCC(B) into yeast.

   Depositor

   Matthew Good

   Article

   Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

   Insert

   PhoCl-TsCC(B)
   

   Tags

   PhoCl fluorophore with C-terminal TsCC(B)

   Expression

   Yeast

   Promoter

   SP6

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAVS1 SA-2A-puro-pA_hSYN1_dTomato-SV40pA

   Plasmid

   #196192

   Purpose

   human synapsin promoter driven dTomato expression at human AAVS1 safe harbour locus

   Depositor

   Jürgen Knoblich

   Article

   Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

   Insert

   2xCHS4_hSYN1_INTRON_dTomato-WPRE-SV40-2xCHS4
   

   Use

   Znf donor

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. ePB-PURO-TT-PA-CRE

   Plasmid

   #179584

   Purpose

   ePiggyBac vector to conditionally express using DOX a light-inducible Cre-recombinase enzyme that takes advantage of the Magnets dimerization system (Magnet-CRE)

   Depositor

   Ali Hemmati Brivanlou

   Article

   De Santis et al Nat Commun. 2021 Nov 19;12(1):6768. doi:

   Insert

   PA-MAG-CRE
   

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Feb. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-H2B-Venus

   Plasmid

   #87614

   Purpose

   Venus (YFP) fused to histone H2B.

   Depositor

   Jin Zhang

   Article

   Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

   Insert

   H2B-Venus (H2BC8 Synthetic, Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSecTag-ATX

    Plasmid

    #17839

    Depositor

    Steven Rosen

    Article

    Kanda et al Nat Immunol. 2008 Apr . 9(4):415-23.

    Insert

    Autotaxin (ENPP2 Human)
    

    Expression

    Mammalian

    Available Since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

11. FAB(ER-mito)

    Plasmid

    #214427

    Purpose

    Detection of ER-mito contact site

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    FRB-V5
    

    Tags

    CFAST10-Halo and RspAN-FRB-V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAV-U6+27-Tornado-F30-Broccoli-Squash-SAM-sensor 4-2

    Plasmid

    #177919

    Purpose

    Expresses ratiometric Squash-SAM sensor 4-2 as circular RNA in mammalian cells using the Tornado expression system.

    Depositor

    Samie Jaffrey

    Article

    Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

    Insert

    Tornado-F30-Broccoli-Squash-SAM-sensor 4-2
    

    Expression

    Mammalian

    Promoter

    U6+27

    Available Since

    Feb. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAV-U6+27-Tornado-F30-Broccoli-Squash-SAM-sensor 5-1

    Plasmid

    #177920

    Purpose

    Expresses ratiometric Squash-SAM sensor 5-1 as circular RNA in mammalian cells using the Tornado expression system.

    Depositor

    Samie Jaffrey

    Article

    Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

    Insert

    Tornado-F30-Broccoli-Squash-SAM-sensor 5-1
    

    Expression

    Mammalian

    Promoter

    U6+27

    Available Since

    Feb. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-LifeAct-FRB-Venus-173-N

    Plasmid

    #87616

    Purpose

    FRB-tagged N-terminal Venus (YFP) fragment for BiFC; localizes to actin cytoskeleton.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    LifeAct-FRB-Venus173-N
    

    Tags

    17-aa sequence from Abp140 (aka "Lifeact&quo…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pfa6a mScarlet-TsCC(B)::KANMX

    Plasmid

    #176397

    Purpose

    Plasmid for cloning mScarlet fused to TsCC(B) coiled coil into yeast.

    Depositor

    Matthew Good

    Article

    Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

    Insert

    mScarlet-TsCC(B)
    

    Tags

    mScarlet fused to TsCC(B)

    Expression

    Bacterial and Yeast

    Promoter

    none

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. SPLICS Po-Mt Short P2A

    Plasmid

    #164116

    Purpose

    Detect the short Peroxisomes-mitochondria contact

    Depositor

    Tito Calì, Marisa Brini

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split GFP Po-Mt Short P2A
    

    Expression

    Mammalian

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL4 mIL-17 2kb promoter

    Plasmid

    #20124

    Depositor

    Warren Strober

    Article

    Fuping et al 2008 Nov;9(11):1297-306. Epub 2008 Oct 1

    Insert

    mIL-17 (Il17a Mouse)
    

    Use

    Luciferase

    Available Since

    Jan. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET22b_PstS_3

    Plasmid

    #78200

    Purpose

    Expression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensor

    Depositor

    Martin Webb

    Article

    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

    Insert

    pstS (A17C,I76G,A197C)
    

    Expression

    Bacterial

    Mutation

    A17C,I76G,A197C (please see depositor comment bel…

    Promoter

    T7

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.tgoGAP1

    Plasmid

    #78737

    Purpose

    Expression of Ca2+ sensor GAP1 in the trans Golgi apparatus

    Depositor

    Teresa Alonso

    Article

    Rodriguez-Garcia et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

    Insert

    tgoGAP1
    

    Tags

    N terminal 81 amino acids of human galactosyltran…

    Expression

    Mammalian

    Mutation

    D117A/D119A/D163A in aequorin moiety

    Promoter

    CMV

    Available Since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pK.myc-MYPT1

    Plasmid

    #24101

    Depositor

    Masumi Eto

    Article

    Eto et al Cell Motil Cytoskeleton. 2005 Oct . 62(2

    Insert

    Myosin-targeting subunit-1 (PPP1R12A Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    S50G, P415Q

    Available Since

    March 19, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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