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ER-HA-mNG21-10

Plasmid

#141165

Purpose

Split Fluorescent Reporter for ER Retrograde Trafficking

Depositor

Wayne Lencer

Article

Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

Insert

ER-HA-mNG21-10-KDEL

Use

Lentiviral

Tags

Signal Sequence, KDEL and HA

Expression

Mammalian

Mutation

K128M, S142T, R150M, G172V and K213M

Promoter

Ef1a

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19 PKC delta

Plasmid

#8427

Depositor

Frederic Mushinski

Article

Mischak et al Biochemistry 1991 Aug 13;30(32):7925-31.

Insert

Mouse Protein Kinase C delta (Prkcd Mouse)

Expression

Bacterial

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only

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pGWB542

Plasmid

#74876

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with eYFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pAGM65881

Plasmid

#153215

Purpose

Level 2 cloning vector for one or several guide RNAs, already contains Cas9 and a kanamycin selection cassette for plant transformation

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-CD81-10

Plasmid

#55281

Purpose

Localization: Tetraspanin/Membrane, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

CD81 (CD81 Human)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pGWB415

Plasmid

#74809

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with 3xHA (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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Ccas9LacZ

Plasmid

#113083

Purpose

Enhanced C. elegans Cas9 vector. Ccas9LacZ contains a lacZ coding sequence, two Cas9 and two type-IIs restriction enzyme recognition sites next to the U6 promoter and gRNA sequences.

Depositor

Tibor Vellai

Article

Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

Type

Empty backbone

Use

CRISPR

Expression

Worm

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Vim-SNAP-tag2

Plasmid

#226518

Purpose

Mamalian cell expression of intermediate filaments (vimentin) fused to SNAP-tag2

Depositor

Kai Johnsson

Article

Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

Insert

Vimentin-SNAP-tag2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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MMLV-CAG-TVA-IRES-Puro

Plasmid

#172366

Purpose

Generation of TVA-expressing stable cell lines, resistant to the antibiotic Puromycin

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

TVA + Puro

Use

Retroviral

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_PDCD6_EF-hand

Plasmid

#110009

Purpose

Protein expression and purification of PDCD6_EF-hand

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PDCD6_EF-hand (PDCD6 Human)

Expression

Bacterial

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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