We narrowed to 39,082 results for: TES

1. pOPINK-TRABID (OTU+AnkUBD, aa 245-697)

   Plasmid

   #61590

   Purpose

   Expresses human TRABID (OTU+AnkUBD domains) in E. coli.

   Depositor

   David Komander

   Article

   Licchesi et al Nat Struct Mol Biol. 2011 Dec 11;19(1):6

   Insert

   TRABID (ZRANB1 Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-244 and aa 698-708.

   Promoter

   T7

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)-ExRai-AKAR2

   Plasmid

   #161753

   Purpose

   Enhanced excitation-ratiometric biosensor for monitoring Protein Kinase A activity in living cells and in vivo.

   Depositor

   Jin Zhang

   Article

   Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

   Insert

   ExRai-AKAR2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. p5E-elavl3

   Plasmid

   #75025

   Purpose

   Can be used to drive pan neuronal expression in zebrafish.

   Depositor

   Nicholas Cole

   Article

   Don et al Zebrafish. 2016 Sep 15.

   Insert

   ELAV like neuron-specific RNA binding protein 3 (elavl3 Zebrafish)
   

   Use

   5' entry vector for multisite gateway three …

   Tags

   Expression

   Mutation

   Promoter

   elavl3

   Available since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. p5E-gfap

   Plasmid

   #75024

   Purpose

   Can be used to drive expression in zebrafish astrocytes.

   Depositor

   Nicholas Cole

   Article

   Don et al Zebrafish. 2016 Sep 15.

   Insert

   glial fibrillary acidic protein promoter (gfap Zebrafish)
   

   Use

   5' entry vector for multisite gateway three …

   Tags

   Expression

   Mutation

   Promoter

   gfap

   Available since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDH-cGAS-E225A D227A-mCherry2-EF1-Puro

   Plasmid

   #132771

   Purpose

   Interacts with cytoplasmic DNA. Useful for identifying sites of nuclear envelope rupture.

   Depositor

   Jan Lammerding

   Article

   Denais et al Science. 2016 Apr 15;352(6283):353-8. do

   Insert

   Cyclic GMP-AMP synthase (CGAS Human)
   

   Use

   Lentiviral

   Tags

   mCherry2

   Expression

   Mammalian

   Mutation

   E225A D227A

   Promoter

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. BNLS

   Plasmid

   #50836

   Purpose

   Expresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 1 (GANLS-DEVD-BNES or RANLS-DEVD-BNES).

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   ddGFP B
   

   Use

   Tags

   triplicated NLS sequence DPKKKRKV

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHDM-Hgpm2

   Plasmid

   #164441

   Purpose

   Encodes codon-optimized HIV gagpol for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

   Insert

   Codon Optimized HIV-1 GagPol
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. huCaV2.2 (pSAD442-1)

   Plasmid

   #62574

   Purpose

   Human CaV2.2 expression in mammalian cells

   Depositor

   Diane Lipscombe

   Article

   Groen et al Hum Mol Genet. 2015 Feb 15;24(4):987-993

   Insert

   CACNA1B (CACNA1B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. EGFP-HsNRF2 (NFE2L2)

   Plasmid

   #194303

   Purpose

   Mammalian expression of human NRF2 (NFE2L2) fused to EGFP at the N-terminus. Parton lab clone KTH

   Depositor

   Rob Parton

   Article

   Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

   Insert

   NFE2L2 (NFE2L2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTet-O-ASCL1-T2A-PuroR

    Plasmid

    #162345

    Purpose

    Lentiviral expression of ASCL1 under the control of the TetON promoter

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    ASCL1 (ASCL1 Human)
    

    Use

    Lentiviral

    Tags

    T2A-PuroR

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-ON

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. mKate2-P2A-APEX2-TAPP1-PH

    Plasmid

    #67662

    Purpose

    Mammalian expression vector driving APEX2-TAPP1 (Human TAPP1-PH domain, [marks PI(3,4)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    mKate2-P2A-APEX2-TAPP1-PH
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA-dCas9-VP64

    Plasmid

    #47107

    Purpose

    Expresses inactivated S. pyogenes dCas9 (D10A, H840A) fused to VP64 transactivator domain in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Insert

    dCas9-VP64
    

    Use

    CRISPR

    Tags

    Flag, HA, SV40 NLS, and VP64

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive)

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAGE-CMV-Luc2-IRES-ZsGreen-W

    Plasmid

    #164432

    Purpose

    Lentiviral vector backbone encoding firefly luciferase and ZsGreen

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

    Inserts

    Luciferase
    ZsGreen
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRK5 FLAG wildtype, catalytic active lipin 1

    Plasmid

    #32005

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2011 Aug 5;146(3):408-20.

    Insert

    lipin1 (Lpin1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRC-CMV-Rev1b

    Plasmid

    #164443

    Purpose

    Encodes HIV-1 Rev accessory protein for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

    Insert

    HIV-1 Rev
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDEF3‐hTIM3 (no tag)

    Plasmid

    #49212

    Purpose

    expresses human Tim3

    Depositor

    Lawrence Kane

    Article

    Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

    Insert

    Tim3 (HAVCR2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV hU6-gRNA hUbC-VP64-dSaCas9-VP64-T2A-Thy1.1

    Plasmid

    #194279

    Purpose

    Expresses gRNA and VP64-dSaCas9-VP64 from lentiviral vector

    Depositor

    Charles Gersbach

    Article

    McCutcheon et al bioRxiv. 2023 May 1:2023.05.01.538906. d

    Inserts

    humanized VP64 dSaCas9 VP64 T2A Thy1.1
    gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 and hUbC

    Available since

    Feb. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHDM-Tat1b

    Plasmid

    #164442

    Purpose

    Encodes HIV-1 Tat accessory protein for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

    Insert

    HIV-1 Tat
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. p5E--3mnx1

    Plasmid

    #74632

    Purpose

    Can be used to drive expression specifically in zebrafish motor neurons

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    motor neuron and pancreas homeobox 1 promoter (mnx1 Zebrafish)
    

    Use

    5' entry vector for multisite gateway three …

    Tags

    Expression

    Mutation

    Promoter

    -3mnx1

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOPINK-OTUB1 (full-length, aa 1-271)

    Plasmid

    #61420

    Purpose

    Expresses human OTUB1 (full-length) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUB1 (Ubiquitin thioesterase OTUB1) (OTUB1 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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